Cargando…

The Significance of the Nucleic Acid Test (NAT) to Prevent Viral Contamination in Musculoskeletal Tissue Transplantation

Objective  The present study aims to highlight the significance of the nucleic acid test (NAT) for musculoskeletal tissue donation and to compare the sensitivity of this test on the different available platforms. Method  The present study is a retrospective survey in a human tissue bank database and...

Descripción completa

Detalles Bibliográficos
Autores principales: Corsi, Carlos Alexandre Curylofo, Luiz, Alan Vinicius Assunção, Cintra, Álefe Saloum, Scarpelini, Katia Carmen Gabriel, Bento, Rodolfo Leandro, Garcia, Flávio Luis, Picado, Celso Herminio Ferraz, Martins, Luís Gustavo Gazoni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Thieme Revinter Publicações Ltda. 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10038709/
https://www.ncbi.nlm.nih.gov/pubmed/36969792
http://dx.doi.org/10.1055/s-0042-1756156
_version_ 1784912141278511104
author Corsi, Carlos Alexandre Curylofo
Luiz, Alan Vinicius Assunção
Cintra, Álefe Saloum
Scarpelini, Katia Carmen Gabriel
Bento, Rodolfo Leandro
Garcia, Flávio Luis
Picado, Celso Herminio Ferraz
Martins, Luís Gustavo Gazoni
author_facet Corsi, Carlos Alexandre Curylofo
Luiz, Alan Vinicius Assunção
Cintra, Álefe Saloum
Scarpelini, Katia Carmen Gabriel
Bento, Rodolfo Leandro
Garcia, Flávio Luis
Picado, Celso Herminio Ferraz
Martins, Luís Gustavo Gazoni
author_sort Corsi, Carlos Alexandre Curylofo
collection PubMed
description Objective  The present study aims to highlight the significance of the nucleic acid test (NAT) for musculoskeletal tissue donation and to compare the sensitivity of this test on the different available platforms. Method  The present study is a retrospective survey in a human tissue bank database and an integrative literature review encompassing the last 10 years. The PubMed portal and the SCOPUS, CINAHL, and Web of Science databases were queried for articles. Results  We found no specific studies on the use and sensitivity of NAT in braindead tissue donors. The information presented in the present study consists of specific contents intended for the Brazilian Blood Transfusion Network (Hemorrede Transfusional Nacional, in Portuguese) and internal retrospective data from a tissue bank located at a city in the state of São Paulo, Brazil. Conclusions  The NAT is effective in blood samples from living patients. However, since biochemical reactions in braindead patients can be different, specific research, platforms, or both are crucial to tissue banks.
format Online
Article
Text
id pubmed-10038709
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Thieme Revinter Publicações Ltda.
record_format MEDLINE/PubMed
spelling pubmed-100387092023-03-25 The Significance of the Nucleic Acid Test (NAT) to Prevent Viral Contamination in Musculoskeletal Tissue Transplantation Corsi, Carlos Alexandre Curylofo Luiz, Alan Vinicius Assunção Cintra, Álefe Saloum Scarpelini, Katia Carmen Gabriel Bento, Rodolfo Leandro Garcia, Flávio Luis Picado, Celso Herminio Ferraz Martins, Luís Gustavo Gazoni Rev Bras Ortop (Sao Paulo) Objective  The present study aims to highlight the significance of the nucleic acid test (NAT) for musculoskeletal tissue donation and to compare the sensitivity of this test on the different available platforms. Method  The present study is a retrospective survey in a human tissue bank database and an integrative literature review encompassing the last 10 years. The PubMed portal and the SCOPUS, CINAHL, and Web of Science databases were queried for articles. Results  We found no specific studies on the use and sensitivity of NAT in braindead tissue donors. The information presented in the present study consists of specific contents intended for the Brazilian Blood Transfusion Network (Hemorrede Transfusional Nacional, in Portuguese) and internal retrospective data from a tissue bank located at a city in the state of São Paulo, Brazil. Conclusions  The NAT is effective in blood samples from living patients. However, since biochemical reactions in braindead patients can be different, specific research, platforms, or both are crucial to tissue banks. Thieme Revinter Publicações Ltda. 2022-10-13 /pmc/articles/PMC10038709/ /pubmed/36969792 http://dx.doi.org/10.1055/s-0042-1756156 Text en Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. ( https://creativecommons.org/licenses/by-nc-nd/4.0/ ) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.
spellingShingle Corsi, Carlos Alexandre Curylofo
Luiz, Alan Vinicius Assunção
Cintra, Álefe Saloum
Scarpelini, Katia Carmen Gabriel
Bento, Rodolfo Leandro
Garcia, Flávio Luis
Picado, Celso Herminio Ferraz
Martins, Luís Gustavo Gazoni
The Significance of the Nucleic Acid Test (NAT) to Prevent Viral Contamination in Musculoskeletal Tissue Transplantation
title The Significance of the Nucleic Acid Test (NAT) to Prevent Viral Contamination in Musculoskeletal Tissue Transplantation
title_full The Significance of the Nucleic Acid Test (NAT) to Prevent Viral Contamination in Musculoskeletal Tissue Transplantation
title_fullStr The Significance of the Nucleic Acid Test (NAT) to Prevent Viral Contamination in Musculoskeletal Tissue Transplantation
title_full_unstemmed The Significance of the Nucleic Acid Test (NAT) to Prevent Viral Contamination in Musculoskeletal Tissue Transplantation
title_short The Significance of the Nucleic Acid Test (NAT) to Prevent Viral Contamination in Musculoskeletal Tissue Transplantation
title_sort significance of the nucleic acid test (nat) to prevent viral contamination in musculoskeletal tissue transplantation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10038709/
https://www.ncbi.nlm.nih.gov/pubmed/36969792
http://dx.doi.org/10.1055/s-0042-1756156
work_keys_str_mv AT corsicarlosalexandrecurylofo thesignificanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT luizalanviniciusassuncao thesignificanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT cintraalefesaloum thesignificanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT scarpelinikatiacarmengabriel thesignificanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT bentorodolfoleandro thesignificanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT garciaflavioluis thesignificanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT picadocelsoherminioferraz thesignificanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT martinsluisgustavogazoni thesignificanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT corsicarlosalexandrecurylofo significanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT luizalanviniciusassuncao significanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT cintraalefesaloum significanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT scarpelinikatiacarmengabriel significanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT bentorodolfoleandro significanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT garciaflavioluis significanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT picadocelsoherminioferraz significanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation
AT martinsluisgustavogazoni significanceofthenucleicacidtestnattopreventviralcontaminationinmusculoskeletaltissuetransplantation