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Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease

Nowadays, the diagnosis and treatment of COPD are often based on the results of lung function tests. Certain individuals, however, are not candidates for lung function testing due to pulmonary bullae, cardiac failure, low lung function, and other factors. Therefore, we evaluated whether serum tyrosi...

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Autores principales: Wang, Decai, Rao, Lizong, Lei, Huiren, Li, Wencui, Yu, Qiufang, Li, Wei, Wei, Jianghong, Xu, Shuyun, Mo, Biwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10039013/
https://www.ncbi.nlm.nih.gov/pubmed/36964173
http://dx.doi.org/10.1038/s41598-023-32096-4
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author Wang, Decai
Rao, Lizong
Lei, Huiren
Li, Wencui
Yu, Qiufang
Li, Wei
Wei, Jianghong
Xu, Shuyun
Mo, Biwen
author_facet Wang, Decai
Rao, Lizong
Lei, Huiren
Li, Wencui
Yu, Qiufang
Li, Wei
Wei, Jianghong
Xu, Shuyun
Mo, Biwen
author_sort Wang, Decai
collection PubMed
description Nowadays, the diagnosis and treatment of COPD are often based on the results of lung function tests. Certain individuals, however, are not candidates for lung function testing due to pulmonary bullae, cardiac failure, low lung function, and other factors. Therefore, we evaluated whether serum tyrosine3-monooxygenase/tryptophan5-monooxygenase activation protein β (14-3-3β) could be a biomarker for the diagnosis of stable COPD patients. The expression of serum 14-3-3β protein was evaluated by an enzyme-linked immunosorbent assay. The association between its concentrations and clinical parameters of stable COPD patients were analyzed by correlation analysis and ROC curve. The results before propensity score matching (PSM) showed that serum 14-3-3β protein concentrations (ng/ml) in stable COPD patients were significantly higher than in healthy controls (P < 0.001). Furthermore, serum 14-3-3β protein concentrations were higher in GOLD 3&4 COPD patients compared with healthy participants, GOLD 1 and GOLD 2 COPD patients (P < 0.05), which shows that the concentration of 14-3-3β protein correlates with disease severity in stable COPD patients. After 1:1 PSM, there was also a statistically significant rise in 14–3-3 protein levels in stable COPD patients compared to healthy controls (P < 0.01). Serum 14-3-3β protein levels were positively correlated with blood neutrophil levels (P < 0.05), and negatively related to lung function parameters in stable COPD patients (P < 0.01). When the cutoff value was set at 29.53 ng/ml, the ROC curve yielded a sensitivity of 84.9% and a specificity of 68.3% for diagnosing stable COPD. The 14-3-3β protein may be a potential serum biomarker for the diagnosis of stable COPD patients, which is associated with disease severity, systemic inflammation, and small airway obstruction.
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spelling pubmed-100390132023-03-26 Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease Wang, Decai Rao, Lizong Lei, Huiren Li, Wencui Yu, Qiufang Li, Wei Wei, Jianghong Xu, Shuyun Mo, Biwen Sci Rep Article Nowadays, the diagnosis and treatment of COPD are often based on the results of lung function tests. Certain individuals, however, are not candidates for lung function testing due to pulmonary bullae, cardiac failure, low lung function, and other factors. Therefore, we evaluated whether serum tyrosine3-monooxygenase/tryptophan5-monooxygenase activation protein β (14-3-3β) could be a biomarker for the diagnosis of stable COPD patients. The expression of serum 14-3-3β protein was evaluated by an enzyme-linked immunosorbent assay. The association between its concentrations and clinical parameters of stable COPD patients were analyzed by correlation analysis and ROC curve. The results before propensity score matching (PSM) showed that serum 14-3-3β protein concentrations (ng/ml) in stable COPD patients were significantly higher than in healthy controls (P < 0.001). Furthermore, serum 14-3-3β protein concentrations were higher in GOLD 3&4 COPD patients compared with healthy participants, GOLD 1 and GOLD 2 COPD patients (P < 0.05), which shows that the concentration of 14-3-3β protein correlates with disease severity in stable COPD patients. After 1:1 PSM, there was also a statistically significant rise in 14–3-3 protein levels in stable COPD patients compared to healthy controls (P < 0.01). Serum 14-3-3β protein levels were positively correlated with blood neutrophil levels (P < 0.05), and negatively related to lung function parameters in stable COPD patients (P < 0.01). When the cutoff value was set at 29.53 ng/ml, the ROC curve yielded a sensitivity of 84.9% and a specificity of 68.3% for diagnosing stable COPD. The 14-3-3β protein may be a potential serum biomarker for the diagnosis of stable COPD patients, which is associated with disease severity, systemic inflammation, and small airway obstruction. Nature Publishing Group UK 2023-03-24 /pmc/articles/PMC10039013/ /pubmed/36964173 http://dx.doi.org/10.1038/s41598-023-32096-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wang, Decai
Rao, Lizong
Lei, Huiren
Li, Wencui
Yu, Qiufang
Li, Wei
Wei, Jianghong
Xu, Shuyun
Mo, Biwen
Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease
title Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease
title_full Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease
title_fullStr Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease
title_full_unstemmed Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease
title_short Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease
title_sort clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10039013/
https://www.ncbi.nlm.nih.gov/pubmed/36964173
http://dx.doi.org/10.1038/s41598-023-32096-4
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