RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding

Progress in sample preparation for scRNA-seq is reported based on RevGel-seq, a reversible-hydrogel technology optimized for samples of fresh cells. Complexes of one cell paired with one barcoded bead are stabilized by a chemical linker and dispersed in a hydrogel in the liquid state. Upon gelation...

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Detalles Bibliográficos
Autores principales: Komatsu, Jun, Cico, Alba, Poncin, Raya, Le Bohec, Maël, Morf, Jörg, Lipin, Stanislav, Graindorge, Antoine, Eckert, Hélène, Saffarian, Azadeh, Cathaly, Léa, Guérin, Frédéric, Majello, Sara, Ulveling, Damien, Vayaboury, Anaïs, Fernandez, Nicolas, Dimitrova, Dilyana, Bussell, Xavier, Fourne, Yannick, Chaumat, Pierre, André, Barbara, Baldivia, Elodie, Godet, Ulysse, Guinin, Mathieu, Moretto, Vivien, Ismail, Joy, Caille, Olivier, Roblot, Natacha, Beaupère, Carine, Liboz, Alexandrine, Guillemain, Ghislaine, Blondeau, Bertrand, Walrafen, Pierre, Edelstein, Stuart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10039079/
https://www.ncbi.nlm.nih.gov/pubmed/36964177
http://dx.doi.org/10.1038/s41598-023-31915-y

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