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Use of green fluorescent nano-sensors for the determination of furosemide in biological samples and pharmaceutical preparations

BACKGROUND: Carbon quantum dots (CQDs) are new class of carbon nanoparticles. Recently, they have been widely used as fluorescent probes due to their easy accessibility, optical properties and chemical inertness. Many available precursors are used in the synthesis of carbon quantum dots. The electri...

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Autores principales: Abo Zaid, Mona H., El-Enany, Nahed, Mostafa, Aziza E., Hadad, Ghada M., Belal, Fathalla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10039510/
https://www.ncbi.nlm.nih.gov/pubmed/36964587
http://dx.doi.org/10.1186/s13065-023-00937-y
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author Abo Zaid, Mona H.
El-Enany, Nahed
Mostafa, Aziza E.
Hadad, Ghada M.
Belal, Fathalla
author_facet Abo Zaid, Mona H.
El-Enany, Nahed
Mostafa, Aziza E.
Hadad, Ghada M.
Belal, Fathalla
author_sort Abo Zaid, Mona H.
collection PubMed
description BACKGROUND: Carbon quantum dots (CQDs) are new class of carbon nanoparticles. Recently, they have been widely used as fluorescent probes due to their easy accessibility, optical properties and chemical inertness. Many available precursors are used in the synthesis of carbon quantum dots. The electrical and optical properties of CQDs could be enhanced by doping hetero atoms such as nitrogen or sulfur into their structure. OBJECTIVE: The current work presents the synthesis and characterization of water-soluble nitrogen doped carbon quantum dots (N-CQDs) and their use as fluorescent nano-sensors for the spectrofluorimetric determination of furosemide in its pharmaceutical preparations and spiked human plasma. METHODS: A domestic microwave was used to prepare the N-CQDs by heating a solution of sucrose and urea till complete charring (about ten minutes). The produced N-CQDs exhibit a strong emission band at 376 nm after excitation at 216 nm. Furosemide caused a quantitative quenching in the fluorescence intensity of the produced N-CQDs. RESULTS: The proposed method was validated according to ICH Guidelines. The method was found to be linear over the range of 0.1–1.0 µg/mL with LOQ of 0.087 µg/ml. CONCLUSION: Ecofriendly nano fluorescent sensors (N-CQDs) were successfully synthesized. The size of N-CQDs was distributed in the range of 6.63 nm to 10.23 nm with an average of 8.2 nm. The produced N-CQDs were used as fluorescent probes for the estimation of furosemide in its pharmaceutical preparations as well as spiked human plasma samples.
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spelling pubmed-100395102023-03-26 Use of green fluorescent nano-sensors for the determination of furosemide in biological samples and pharmaceutical preparations Abo Zaid, Mona H. El-Enany, Nahed Mostafa, Aziza E. Hadad, Ghada M. Belal, Fathalla BMC Chem Research BACKGROUND: Carbon quantum dots (CQDs) are new class of carbon nanoparticles. Recently, they have been widely used as fluorescent probes due to their easy accessibility, optical properties and chemical inertness. Many available precursors are used in the synthesis of carbon quantum dots. The electrical and optical properties of CQDs could be enhanced by doping hetero atoms such as nitrogen or sulfur into their structure. OBJECTIVE: The current work presents the synthesis and characterization of water-soluble nitrogen doped carbon quantum dots (N-CQDs) and their use as fluorescent nano-sensors for the spectrofluorimetric determination of furosemide in its pharmaceutical preparations and spiked human plasma. METHODS: A domestic microwave was used to prepare the N-CQDs by heating a solution of sucrose and urea till complete charring (about ten minutes). The produced N-CQDs exhibit a strong emission band at 376 nm after excitation at 216 nm. Furosemide caused a quantitative quenching in the fluorescence intensity of the produced N-CQDs. RESULTS: The proposed method was validated according to ICH Guidelines. The method was found to be linear over the range of 0.1–1.0 µg/mL with LOQ of 0.087 µg/ml. CONCLUSION: Ecofriendly nano fluorescent sensors (N-CQDs) were successfully synthesized. The size of N-CQDs was distributed in the range of 6.63 nm to 10.23 nm with an average of 8.2 nm. The produced N-CQDs were used as fluorescent probes for the estimation of furosemide in its pharmaceutical preparations as well as spiked human plasma samples. Springer International Publishing 2023-03-24 /pmc/articles/PMC10039510/ /pubmed/36964587 http://dx.doi.org/10.1186/s13065-023-00937-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Abo Zaid, Mona H.
El-Enany, Nahed
Mostafa, Aziza E.
Hadad, Ghada M.
Belal, Fathalla
Use of green fluorescent nano-sensors for the determination of furosemide in biological samples and pharmaceutical preparations
title Use of green fluorescent nano-sensors for the determination of furosemide in biological samples and pharmaceutical preparations
title_full Use of green fluorescent nano-sensors for the determination of furosemide in biological samples and pharmaceutical preparations
title_fullStr Use of green fluorescent nano-sensors for the determination of furosemide in biological samples and pharmaceutical preparations
title_full_unstemmed Use of green fluorescent nano-sensors for the determination of furosemide in biological samples and pharmaceutical preparations
title_short Use of green fluorescent nano-sensors for the determination of furosemide in biological samples and pharmaceutical preparations
title_sort use of green fluorescent nano-sensors for the determination of furosemide in biological samples and pharmaceutical preparations
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10039510/
https://www.ncbi.nlm.nih.gov/pubmed/36964587
http://dx.doi.org/10.1186/s13065-023-00937-y
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