Complete sequence verification of plasmid DNA using the Oxford Nanopore Technologies’ MinION device

BACKGROUND: Sequence verification is essential for plasmids used as critical reagents or therapeutic products. Typically, high-quality plasmid sequence is achieved through capillary-based Sanger sequencing, requiring customized sets of primers for each plasmid. This process can become expensive, par...

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Detalles Bibliográficos
Autores principales: Brown, Scott D., Dreolini, Lisa, Wilson, Jessica F., Balasundaram, Miruna, Holt, Robert A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10039527/
https://www.ncbi.nlm.nih.gov/pubmed/36964503
http://dx.doi.org/10.1186/s12859-023-05226-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10039527/
https://www.ncbi.nlm.nih.gov/pubmed/36964503
http://dx.doi.org/10.1186/s12859-023-05226-y

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