Perspective on the application of genome sequencing for monkeypox virus surveillance

• Whole genome sequencing of MPXV is crucial for monitoring emerging variants and assessing their potential pathogenicity. • The critical steps of mNGS, encompassing nucleic acid extraction, library preparation, sequencing, and data analysis, are concisely explained. • Optimization strategies for sa...

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Detalles Bibliográficos
Autores principales: Chen, Yuda, Wu, Changcheng, A, Ruhan, Zhao, Li, Zhang, Zhongxian, Tan, Wenjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2023
Materias:
Perspective
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10039704/
https://www.ncbi.nlm.nih.gov/pubmed/36972867
http://dx.doi.org/10.1016/j.virs.2023.03.006
Descripción
Sumario:• Whole genome sequencing of MPXV is crucial for monitoring emerging variants and assessing their potential pathogenicity. • The critical steps of mNGS, encompassing nucleic acid extraction, library preparation, sequencing, and data analysis, are concisely explained. • Optimization strategies for sample pre-processing, virus enrichment, and sequencing platform selection are deliberated. • Conducting next-generation sequencing and third-generation sequencing concurrently is highly recommended.

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