Perspective on the application of genome sequencing for monkeypox virus surveillance

• Whole genome sequencing of MPXV is crucial for monitoring emerging variants and assessing their potential pathogenicity. • The critical steps of mNGS, encompassing nucleic acid extraction, library preparation, sequencing, and data analysis, are concisely explained. • Optimization strategies for sa...

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Detalles Bibliográficos
Autores principales: Chen, Yuda, Wu, Changcheng, A, Ruhan, Zhao, Li, Zhang, Zhongxian, Tan, Wenjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2023
Materias:
Perspective
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10039704/
https://www.ncbi.nlm.nih.gov/pubmed/36972867
http://dx.doi.org/10.1016/j.virs.2023.03.006
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author Chen, Yuda
Wu, Changcheng
A, Ruhan
Zhao, Li
Zhang, Zhongxian
Tan, Wenjie
author_facet Chen, Yuda
Wu, Changcheng
A, Ruhan
Zhao, Li
Zhang, Zhongxian
Tan, Wenjie
author_sort Chen, Yuda
collection PubMed
description • Whole genome sequencing of MPXV is crucial for monitoring emerging variants and assessing their potential pathogenicity. • The critical steps of mNGS, encompassing nucleic acid extraction, library preparation, sequencing, and data analysis, are concisely explained. • Optimization strategies for sample pre-processing, virus enrichment, and sequencing platform selection are deliberated. • Conducting next-generation sequencing and third-generation sequencing concurrently is highly recommended.
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spelling pubmed-100397042023-03-26 Perspective on the application of genome sequencing for monkeypox virus surveillance Chen, Yuda Wu, Changcheng A, Ruhan Zhao, Li Zhang, Zhongxian Tan, Wenjie Virol Sin Perspective • Whole genome sequencing of MPXV is crucial for monitoring emerging variants and assessing their potential pathogenicity. • The critical steps of mNGS, encompassing nucleic acid extraction, library preparation, sequencing, and data analysis, are concisely explained. • Optimization strategies for sample pre-processing, virus enrichment, and sequencing platform selection are deliberated. • Conducting next-generation sequencing and third-generation sequencing concurrently is highly recommended. Wuhan Institute of Virology, Chinese Academy of Sciences 2023-03-25 /pmc/articles/PMC10039704/ /pubmed/36972867 http://dx.doi.org/10.1016/j.virs.2023.03.006 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Perspective
Chen, Yuda
Wu, Changcheng
A, Ruhan
Zhao, Li
Zhang, Zhongxian
Tan, Wenjie
Perspective on the application of genome sequencing for monkeypox virus surveillance
title Perspective on the application of genome sequencing for monkeypox virus surveillance
title_full Perspective on the application of genome sequencing for monkeypox virus surveillance
title_fullStr Perspective on the application of genome sequencing for monkeypox virus surveillance
title_full_unstemmed Perspective on the application of genome sequencing for monkeypox virus surveillance
title_short Perspective on the application of genome sequencing for monkeypox virus surveillance
title_sort perspective on the application of genome sequencing for monkeypox virus surveillance
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10039704/
https://www.ncbi.nlm.nih.gov/pubmed/36972867
http://dx.doi.org/10.1016/j.virs.2023.03.006
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