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Circ_0003340 regulates the expression of ENAH to affect the development of esophageal cancer through miR‐874‐3p

BACKGROUND: Esophageal cancer is a malignant tumor with a poor prognosis and high incidence. Circular RNAs (circRNAs) have been shown to be involved in the pathogenesis of cancers, including esophageal cancer. Here, we explored the precise role of circ_0003340 in esophageal cancer development. METHO...

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Autores principales: Wang, Jingyi, Zhao, Ning, Peng, Shengzu, Zhang, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Australia, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10040281/
https://www.ncbi.nlm.nih.gov/pubmed/36737402
http://dx.doi.org/10.1111/1759-7714.14812
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author Wang, Jingyi
Zhao, Ning
Peng, Shengzu
Zhang, Tao
author_facet Wang, Jingyi
Zhao, Ning
Peng, Shengzu
Zhang, Tao
author_sort Wang, Jingyi
collection PubMed
description BACKGROUND: Esophageal cancer is a malignant tumor with a poor prognosis and high incidence. Circular RNAs (circRNAs) have been shown to be involved in the pathogenesis of cancers, including esophageal cancer. Here, we explored the precise role of circ_0003340 in esophageal cancer development. METHODS: The expression levels of circ_0003340, miR‐874‐3p and enabled homolog (ENAH) were detected by quantitative real‐time polymerase chain reaction and western blot. Subcellular localization and RNase R assays were used to characterize circ_0003340. Cell Counting Kit 8, flow cytometry, transwell assays were used to analyze cell proliferation, apoptosis, migration and invasion. The effect of circ_0003340 on tumor growth was assessed by tumor experiments in vivo. Dual‐luciferase reporter assay was used to analyze the relationship between miR‐874‐3p and circ_0003340 or ENAH. RESULTS: Circ_0003340 was mainly located in the cytoplasm and was upregulated in esophageal cancer tissues and cells. Circ_0003340 knockdown inhibited cell proliferation, migration, invasion, glucose consumption, and lactate production and induced cell apoptosis in esophageal cancer cells. Moreover, circ_0003340 knockdown suppressed tumor growth in vivo. MiR‐874‐3p was reduced in esophageal cancer tissues and cells, and it was a molecular mediator of circ_0003340 function in esophageal cancer cells. ENAH was identified as a direct and functional target of miR‐874‐3p in esophageal cancer cells. The promotion effect of circ_0003340 on ENAH was ameliorated by miR‐874‐3p. CONCLUSION: The data demonstrated that circ_0003340 promoted the progression of esophageal cancer through miR‐874‐3p/ENAH axis, which might provide novel therapeutic targets for esophageal cancer intervention.
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spelling pubmed-100402812023-03-27 Circ_0003340 regulates the expression of ENAH to affect the development of esophageal cancer through miR‐874‐3p Wang, Jingyi Zhao, Ning Peng, Shengzu Zhang, Tao Thorac Cancer Original Articles BACKGROUND: Esophageal cancer is a malignant tumor with a poor prognosis and high incidence. Circular RNAs (circRNAs) have been shown to be involved in the pathogenesis of cancers, including esophageal cancer. Here, we explored the precise role of circ_0003340 in esophageal cancer development. METHODS: The expression levels of circ_0003340, miR‐874‐3p and enabled homolog (ENAH) were detected by quantitative real‐time polymerase chain reaction and western blot. Subcellular localization and RNase R assays were used to characterize circ_0003340. Cell Counting Kit 8, flow cytometry, transwell assays were used to analyze cell proliferation, apoptosis, migration and invasion. The effect of circ_0003340 on tumor growth was assessed by tumor experiments in vivo. Dual‐luciferase reporter assay was used to analyze the relationship between miR‐874‐3p and circ_0003340 or ENAH. RESULTS: Circ_0003340 was mainly located in the cytoplasm and was upregulated in esophageal cancer tissues and cells. Circ_0003340 knockdown inhibited cell proliferation, migration, invasion, glucose consumption, and lactate production and induced cell apoptosis in esophageal cancer cells. Moreover, circ_0003340 knockdown suppressed tumor growth in vivo. MiR‐874‐3p was reduced in esophageal cancer tissues and cells, and it was a molecular mediator of circ_0003340 function in esophageal cancer cells. ENAH was identified as a direct and functional target of miR‐874‐3p in esophageal cancer cells. The promotion effect of circ_0003340 on ENAH was ameliorated by miR‐874‐3p. CONCLUSION: The data demonstrated that circ_0003340 promoted the progression of esophageal cancer through miR‐874‐3p/ENAH axis, which might provide novel therapeutic targets for esophageal cancer intervention. John Wiley & Sons Australia, Ltd 2023-02-03 /pmc/articles/PMC10040281/ /pubmed/36737402 http://dx.doi.org/10.1111/1759-7714.14812 Text en © 2023 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Wang, Jingyi
Zhao, Ning
Peng, Shengzu
Zhang, Tao
Circ_0003340 regulates the expression of ENAH to affect the development of esophageal cancer through miR‐874‐3p
title Circ_0003340 regulates the expression of ENAH to affect the development of esophageal cancer through miR‐874‐3p
title_full Circ_0003340 regulates the expression of ENAH to affect the development of esophageal cancer through miR‐874‐3p
title_fullStr Circ_0003340 regulates the expression of ENAH to affect the development of esophageal cancer through miR‐874‐3p
title_full_unstemmed Circ_0003340 regulates the expression of ENAH to affect the development of esophageal cancer through miR‐874‐3p
title_short Circ_0003340 regulates the expression of ENAH to affect the development of esophageal cancer through miR‐874‐3p
title_sort circ_0003340 regulates the expression of enah to affect the development of esophageal cancer through mir‐874‐3p
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10040281/
https://www.ncbi.nlm.nih.gov/pubmed/36737402
http://dx.doi.org/10.1111/1759-7714.14812
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