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Understanding the mechanisms of halotolerance in members of Pontixanthobacter and Allopontixanthobacter by comparative genome analysis

Halotolerant microorganisms have developed versatile mechanisms for coping with saline stress. With the increasing number of isolated halotolerant strains and their genomes being sequenced, comparative genome analysis would help understand the mechanisms of salt tolerance. Six type strains of Pontix...

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Autores principales: Zhou, Peng, Bu, Yu-Xin, Xu, Lin, Xu, Xue-Wei, Shen, Hong-Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10040529/
https://www.ncbi.nlm.nih.gov/pubmed/36992937
http://dx.doi.org/10.3389/fmicb.2023.1111472
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author Zhou, Peng
Bu, Yu-Xin
Xu, Lin
Xu, Xue-Wei
Shen, Hong-Bin
author_facet Zhou, Peng
Bu, Yu-Xin
Xu, Lin
Xu, Xue-Wei
Shen, Hong-Bin
author_sort Zhou, Peng
collection PubMed
description Halotolerant microorganisms have developed versatile mechanisms for coping with saline stress. With the increasing number of isolated halotolerant strains and their genomes being sequenced, comparative genome analysis would help understand the mechanisms of salt tolerance. Six type strains of Pontixanthobacter and Allopontixanthobacter, two phylogenetically close genera, were isolated from diverse salty environments and showed different NaCl tolerances, from 3 to 10% (w/v). Based on the co-occurrence greater than 0.8 between halotolerance and open reading frame (ORF) among the six strains, possible explanations for halotolerance were discussed regarding osmolyte, membrane permeability, transportation, intracellular signaling, polysaccharide biosynthesis, and SOS response, which provided hypotheses for further investigations. The strategy of analyzing genome-wide co-occurrence between genetic diversity and physiological characteristics sheds light on how microorganisms adapt to the environment.
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spelling pubmed-100405292023-03-28 Understanding the mechanisms of halotolerance in members of Pontixanthobacter and Allopontixanthobacter by comparative genome analysis Zhou, Peng Bu, Yu-Xin Xu, Lin Xu, Xue-Wei Shen, Hong-Bin Front Microbiol Microbiology Halotolerant microorganisms have developed versatile mechanisms for coping with saline stress. With the increasing number of isolated halotolerant strains and their genomes being sequenced, comparative genome analysis would help understand the mechanisms of salt tolerance. Six type strains of Pontixanthobacter and Allopontixanthobacter, two phylogenetically close genera, were isolated from diverse salty environments and showed different NaCl tolerances, from 3 to 10% (w/v). Based on the co-occurrence greater than 0.8 between halotolerance and open reading frame (ORF) among the six strains, possible explanations for halotolerance were discussed regarding osmolyte, membrane permeability, transportation, intracellular signaling, polysaccharide biosynthesis, and SOS response, which provided hypotheses for further investigations. The strategy of analyzing genome-wide co-occurrence between genetic diversity and physiological characteristics sheds light on how microorganisms adapt to the environment. Frontiers Media S.A. 2023-03-13 /pmc/articles/PMC10040529/ /pubmed/36992937 http://dx.doi.org/10.3389/fmicb.2023.1111472 Text en Copyright © 2023 Zhou, Bu, Xu, Xu and Shen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhou, Peng
Bu, Yu-Xin
Xu, Lin
Xu, Xue-Wei
Shen, Hong-Bin
Understanding the mechanisms of halotolerance in members of Pontixanthobacter and Allopontixanthobacter by comparative genome analysis
title Understanding the mechanisms of halotolerance in members of Pontixanthobacter and Allopontixanthobacter by comparative genome analysis
title_full Understanding the mechanisms of halotolerance in members of Pontixanthobacter and Allopontixanthobacter by comparative genome analysis
title_fullStr Understanding the mechanisms of halotolerance in members of Pontixanthobacter and Allopontixanthobacter by comparative genome analysis
title_full_unstemmed Understanding the mechanisms of halotolerance in members of Pontixanthobacter and Allopontixanthobacter by comparative genome analysis
title_short Understanding the mechanisms of halotolerance in members of Pontixanthobacter and Allopontixanthobacter by comparative genome analysis
title_sort understanding the mechanisms of halotolerance in members of pontixanthobacter and allopontixanthobacter by comparative genome analysis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10040529/
https://www.ncbi.nlm.nih.gov/pubmed/36992937
http://dx.doi.org/10.3389/fmicb.2023.1111472
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