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Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos

The present study investigated the effects of epidermal growth factors (EGF) and/or β-Mercaptoethanol (βME) supplementations to oocyte maturation, fertilization, and culture media on the buffalo in vitro embryo production. The ovaries were collected and transferred within 2 h to the laboratory. The...

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Autores principales: Ahmed, Ahmed Ezzat, Sindi, Ramya Ahmad, Yousef, Nasra Ahmed, Hussein, Hassan A., Badr, Magdy R., Syaad, Khalid M. Al, Al-Saeed, Fatimah A., Hassaneen, Ahmed Saad A., Abdelrahman, Mohamed, Ali, Montaser Elsayed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10040534/
https://www.ncbi.nlm.nih.gov/pubmed/36992972
http://dx.doi.org/10.3389/fvets.2023.1138220
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author Ahmed, Ahmed Ezzat
Sindi, Ramya Ahmad
Yousef, Nasra Ahmed
Hussein, Hassan A.
Badr, Magdy R.
Syaad, Khalid M. Al
Al-Saeed, Fatimah A.
Hassaneen, Ahmed Saad A.
Abdelrahman, Mohamed
Ali, Montaser Elsayed
author_facet Ahmed, Ahmed Ezzat
Sindi, Ramya Ahmad
Yousef, Nasra Ahmed
Hussein, Hassan A.
Badr, Magdy R.
Syaad, Khalid M. Al
Al-Saeed, Fatimah A.
Hassaneen, Ahmed Saad A.
Abdelrahman, Mohamed
Ali, Montaser Elsayed
author_sort Ahmed, Ahmed Ezzat
collection PubMed
description The present study investigated the effects of epidermal growth factors (EGF) and/or β-Mercaptoethanol (βME) supplementations to oocyte maturation, fertilization, and culture media on the buffalo in vitro embryo production. The ovaries were collected and transferred within 2 h to the laboratory. The cumulus oocytes complexes were aspirated from 3 to 8 mm diameter follicles. Firstly, EGF; 0, 10, 20, or 50 ng/mL or βME; 0, 25, 50, 100, or 200 μM were supplemented to the in vitro maturation (TCM-199), fertilization (IVF-TALP), or culture (IVC: SOF) media. Our results revealed that supplementing EGF (20 ng/mL) to the TCM-199, IVF-TALP, or SOF media could efficiently improve the growth rates and development of buffalos' embryos, while EGF (50 ng/mL) could stimulate the embryo production only after treatment of the IVF-TALP /or SOF media, but not the IVM medium. However, βME was less efficient than EGF; it stimulated the growth rates of buffalo embryos when supplemented with the maturation and fertilization (IVF-TALP) media in a 50 μM concentration. Secondly, combined EGF (20 ng/mL) and βME (50 μM) were supplemented to the maturation media as effective concentration. The combined treatment of EGF (20 ng/mL) and βME (50 μM) showed no significant enhancing effect on the buffalo embryos compared to each alone. For future perspectives, further study is required to examine the effects of combined EGF and βME on the maturation and fertilization of buffalo oocytes at different categories of age and seasonal localities.
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spelling pubmed-100405342023-03-28 Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos Ahmed, Ahmed Ezzat Sindi, Ramya Ahmad Yousef, Nasra Ahmed Hussein, Hassan A. Badr, Magdy R. Syaad, Khalid M. Al Al-Saeed, Fatimah A. Hassaneen, Ahmed Saad A. Abdelrahman, Mohamed Ali, Montaser Elsayed Front Vet Sci Veterinary Science The present study investigated the effects of epidermal growth factors (EGF) and/or β-Mercaptoethanol (βME) supplementations to oocyte maturation, fertilization, and culture media on the buffalo in vitro embryo production. The ovaries were collected and transferred within 2 h to the laboratory. The cumulus oocytes complexes were aspirated from 3 to 8 mm diameter follicles. Firstly, EGF; 0, 10, 20, or 50 ng/mL or βME; 0, 25, 50, 100, or 200 μM were supplemented to the in vitro maturation (TCM-199), fertilization (IVF-TALP), or culture (IVC: SOF) media. Our results revealed that supplementing EGF (20 ng/mL) to the TCM-199, IVF-TALP, or SOF media could efficiently improve the growth rates and development of buffalos' embryos, while EGF (50 ng/mL) could stimulate the embryo production only after treatment of the IVF-TALP /or SOF media, but not the IVM medium. However, βME was less efficient than EGF; it stimulated the growth rates of buffalo embryos when supplemented with the maturation and fertilization (IVF-TALP) media in a 50 μM concentration. Secondly, combined EGF (20 ng/mL) and βME (50 μM) were supplemented to the maturation media as effective concentration. The combined treatment of EGF (20 ng/mL) and βME (50 μM) showed no significant enhancing effect on the buffalo embryos compared to each alone. For future perspectives, further study is required to examine the effects of combined EGF and βME on the maturation and fertilization of buffalo oocytes at different categories of age and seasonal localities. Frontiers Media S.A. 2023-03-13 /pmc/articles/PMC10040534/ /pubmed/36992972 http://dx.doi.org/10.3389/fvets.2023.1138220 Text en Copyright © 2023 Ahmed, Sindi, Yousef, Hussein, Badr, Syaad, Al-Saeed, Hassaneen, Abdelrahman and Ali. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Ahmed, Ahmed Ezzat
Sindi, Ramya Ahmad
Yousef, Nasra Ahmed
Hussein, Hassan A.
Badr, Magdy R.
Syaad, Khalid M. Al
Al-Saeed, Fatimah A.
Hassaneen, Ahmed Saad A.
Abdelrahman, Mohamed
Ali, Montaser Elsayed
Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos
title Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos
title_full Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos
title_fullStr Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos
title_full_unstemmed Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos
title_short Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos
title_sort impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10040534/
https://www.ncbi.nlm.nih.gov/pubmed/36992972
http://dx.doi.org/10.3389/fvets.2023.1138220
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