Cargando…
Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos
The present study investigated the effects of epidermal growth factors (EGF) and/or β-Mercaptoethanol (βME) supplementations to oocyte maturation, fertilization, and culture media on the buffalo in vitro embryo production. The ovaries were collected and transferred within 2 h to the laboratory. The...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10040534/ https://www.ncbi.nlm.nih.gov/pubmed/36992972 http://dx.doi.org/10.3389/fvets.2023.1138220 |
_version_ | 1784912493595852800 |
---|---|
author | Ahmed, Ahmed Ezzat Sindi, Ramya Ahmad Yousef, Nasra Ahmed Hussein, Hassan A. Badr, Magdy R. Syaad, Khalid M. Al Al-Saeed, Fatimah A. Hassaneen, Ahmed Saad A. Abdelrahman, Mohamed Ali, Montaser Elsayed |
author_facet | Ahmed, Ahmed Ezzat Sindi, Ramya Ahmad Yousef, Nasra Ahmed Hussein, Hassan A. Badr, Magdy R. Syaad, Khalid M. Al Al-Saeed, Fatimah A. Hassaneen, Ahmed Saad A. Abdelrahman, Mohamed Ali, Montaser Elsayed |
author_sort | Ahmed, Ahmed Ezzat |
collection | PubMed |
description | The present study investigated the effects of epidermal growth factors (EGF) and/or β-Mercaptoethanol (βME) supplementations to oocyte maturation, fertilization, and culture media on the buffalo in vitro embryo production. The ovaries were collected and transferred within 2 h to the laboratory. The cumulus oocytes complexes were aspirated from 3 to 8 mm diameter follicles. Firstly, EGF; 0, 10, 20, or 50 ng/mL or βME; 0, 25, 50, 100, or 200 μM were supplemented to the in vitro maturation (TCM-199), fertilization (IVF-TALP), or culture (IVC: SOF) media. Our results revealed that supplementing EGF (20 ng/mL) to the TCM-199, IVF-TALP, or SOF media could efficiently improve the growth rates and development of buffalos' embryos, while EGF (50 ng/mL) could stimulate the embryo production only after treatment of the IVF-TALP /or SOF media, but not the IVM medium. However, βME was less efficient than EGF; it stimulated the growth rates of buffalo embryos when supplemented with the maturation and fertilization (IVF-TALP) media in a 50 μM concentration. Secondly, combined EGF (20 ng/mL) and βME (50 μM) were supplemented to the maturation media as effective concentration. The combined treatment of EGF (20 ng/mL) and βME (50 μM) showed no significant enhancing effect on the buffalo embryos compared to each alone. For future perspectives, further study is required to examine the effects of combined EGF and βME on the maturation and fertilization of buffalo oocytes at different categories of age and seasonal localities. |
format | Online Article Text |
id | pubmed-10040534 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100405342023-03-28 Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos Ahmed, Ahmed Ezzat Sindi, Ramya Ahmad Yousef, Nasra Ahmed Hussein, Hassan A. Badr, Magdy R. Syaad, Khalid M. Al Al-Saeed, Fatimah A. Hassaneen, Ahmed Saad A. Abdelrahman, Mohamed Ali, Montaser Elsayed Front Vet Sci Veterinary Science The present study investigated the effects of epidermal growth factors (EGF) and/or β-Mercaptoethanol (βME) supplementations to oocyte maturation, fertilization, and culture media on the buffalo in vitro embryo production. The ovaries were collected and transferred within 2 h to the laboratory. The cumulus oocytes complexes were aspirated from 3 to 8 mm diameter follicles. Firstly, EGF; 0, 10, 20, or 50 ng/mL or βME; 0, 25, 50, 100, or 200 μM were supplemented to the in vitro maturation (TCM-199), fertilization (IVF-TALP), or culture (IVC: SOF) media. Our results revealed that supplementing EGF (20 ng/mL) to the TCM-199, IVF-TALP, or SOF media could efficiently improve the growth rates and development of buffalos' embryos, while EGF (50 ng/mL) could stimulate the embryo production only after treatment of the IVF-TALP /or SOF media, but not the IVM medium. However, βME was less efficient than EGF; it stimulated the growth rates of buffalo embryos when supplemented with the maturation and fertilization (IVF-TALP) media in a 50 μM concentration. Secondly, combined EGF (20 ng/mL) and βME (50 μM) were supplemented to the maturation media as effective concentration. The combined treatment of EGF (20 ng/mL) and βME (50 μM) showed no significant enhancing effect on the buffalo embryos compared to each alone. For future perspectives, further study is required to examine the effects of combined EGF and βME on the maturation and fertilization of buffalo oocytes at different categories of age and seasonal localities. Frontiers Media S.A. 2023-03-13 /pmc/articles/PMC10040534/ /pubmed/36992972 http://dx.doi.org/10.3389/fvets.2023.1138220 Text en Copyright © 2023 Ahmed, Sindi, Yousef, Hussein, Badr, Syaad, Al-Saeed, Hassaneen, Abdelrahman and Ali. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Ahmed, Ahmed Ezzat Sindi, Ramya Ahmad Yousef, Nasra Ahmed Hussein, Hassan A. Badr, Magdy R. Syaad, Khalid M. Al Al-Saeed, Fatimah A. Hassaneen, Ahmed Saad A. Abdelrahman, Mohamed Ali, Montaser Elsayed Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos |
title | Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos |
title_full | Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos |
title_fullStr | Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos |
title_full_unstemmed | Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos |
title_short | Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos |
title_sort | impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10040534/ https://www.ncbi.nlm.nih.gov/pubmed/36992972 http://dx.doi.org/10.3389/fvets.2023.1138220 |
work_keys_str_mv | AT ahmedahmedezzat impactofepidermalgrowthfactorandorbmercaptoethanolsupplementationsontheinvitroproducedbuffaloesembryos AT sindiramyaahmad impactofepidermalgrowthfactorandorbmercaptoethanolsupplementationsontheinvitroproducedbuffaloesembryos AT yousefnasraahmed impactofepidermalgrowthfactorandorbmercaptoethanolsupplementationsontheinvitroproducedbuffaloesembryos AT husseinhassana impactofepidermalgrowthfactorandorbmercaptoethanolsupplementationsontheinvitroproducedbuffaloesembryos AT badrmagdyr impactofepidermalgrowthfactorandorbmercaptoethanolsupplementationsontheinvitroproducedbuffaloesembryos AT syaadkhalidmal impactofepidermalgrowthfactorandorbmercaptoethanolsupplementationsontheinvitroproducedbuffaloesembryos AT alsaeedfatimaha impactofepidermalgrowthfactorandorbmercaptoethanolsupplementationsontheinvitroproducedbuffaloesembryos AT hassaneenahmedsaada impactofepidermalgrowthfactorandorbmercaptoethanolsupplementationsontheinvitroproducedbuffaloesembryos AT abdelrahmanmohamed impactofepidermalgrowthfactorandorbmercaptoethanolsupplementationsontheinvitroproducedbuffaloesembryos AT alimontaserelsayed impactofepidermalgrowthfactorandorbmercaptoethanolsupplementationsontheinvitroproducedbuffaloesembryos |