A general method for quantitative fractionation of mammalian cells

Subcellular fractionation in combination with mass spectrometry–based proteomics is a powerful tool to study localization of key proteins in health and disease. Here we offered a reliable and rapid method for mammalian cell fractionation, tuned for such proteomic analyses. This method proves readily...

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Detalles Bibliográficos
Autores principales: Udi, Yael, Zhang, Wenzhu, Stein, Milana E., Ricardo-Lax, Inna, Pasolli, Hilda A., Chait, Brian T., Rout, Michael P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2023
Materias:
Tools
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10040634/
https://www.ncbi.nlm.nih.gov/pubmed/36920247
http://dx.doi.org/10.1083/jcb.202209062
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author Udi, Yael
Zhang, Wenzhu
Stein, Milana E.
Ricardo-Lax, Inna
Pasolli, Hilda A.
Chait, Brian T.
Rout, Michael P.
author_facet Udi, Yael
Zhang, Wenzhu
Stein, Milana E.
Ricardo-Lax, Inna
Pasolli, Hilda A.
Chait, Brian T.
Rout, Michael P.
author_sort Udi, Yael
collection PubMed
description Subcellular fractionation in combination with mass spectrometry–based proteomics is a powerful tool to study localization of key proteins in health and disease. Here we offered a reliable and rapid method for mammalian cell fractionation, tuned for such proteomic analyses. This method proves readily applicable to different cell lines in which all the cellular contents are accounted for, while maintaining nuclear and nuclear envelope integrity. We demonstrated the method’s utility by quantifying the effects of a nuclear export inhibitor on nucleoplasmic and cytoplasmic proteomes.
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spelling pubmed-100406342023-03-28 A general method for quantitative fractionation of mammalian cells Udi, Yael Zhang, Wenzhu Stein, Milana E. Ricardo-Lax, Inna Pasolli, Hilda A. Chait, Brian T. Rout, Michael P. J Cell Biol Tools Subcellular fractionation in combination with mass spectrometry–based proteomics is a powerful tool to study localization of key proteins in health and disease. Here we offered a reliable and rapid method for mammalian cell fractionation, tuned for such proteomic analyses. This method proves readily applicable to different cell lines in which all the cellular contents are accounted for, while maintaining nuclear and nuclear envelope integrity. We demonstrated the method’s utility by quantifying the effects of a nuclear export inhibitor on nucleoplasmic and cytoplasmic proteomes. Rockefeller University Press 2023-03-15 /pmc/articles/PMC10040634/ /pubmed/36920247 http://dx.doi.org/10.1083/jcb.202209062 Text en © 2023 Udi et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Tools
Udi, Yael
Zhang, Wenzhu
Stein, Milana E.
Ricardo-Lax, Inna
Pasolli, Hilda A.
Chait, Brian T.
Rout, Michael P.
A general method for quantitative fractionation of mammalian cells
title A general method for quantitative fractionation of mammalian cells
title_full A general method for quantitative fractionation of mammalian cells
title_fullStr A general method for quantitative fractionation of mammalian cells
title_full_unstemmed A general method for quantitative fractionation of mammalian cells
title_short A general method for quantitative fractionation of mammalian cells
title_sort general method for quantitative fractionation of mammalian cells
topic Tools
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10040634/
https://www.ncbi.nlm.nih.gov/pubmed/36920247
http://dx.doi.org/10.1083/jcb.202209062
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