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A general method for quantitative fractionation of mammalian cells

Subcellular fractionation in combination with mass spectrometry–based proteomics is a powerful tool to study localization of key proteins in health and disease. Here we offered a reliable and rapid method for mammalian cell fractionation, tuned for such proteomic analyses. This method proves readily...

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Detalles Bibliográficos
Autores principales: Udi, Yael, Zhang, Wenzhu, Stein, Milana E., Ricardo-Lax, Inna, Pasolli, Hilda A., Chait, Brian T., Rout, Michael P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10040634/
https://www.ncbi.nlm.nih.gov/pubmed/36920247
http://dx.doi.org/10.1083/jcb.202209062