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Terahertz time-domain attenuated total reflection spectroscopy integrated with a microfluidic chip

The integration of a microfluidic chip into terahertz time-domain attenuated total reflection (THz TD-ATR) spectroscopy is highly demanded for the accurate measurement of aqueous samples. Hitherto, however little work has been reported on this regard. Here, we demonstrate a strategy of fabricating a...

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Autores principales: Fu, Ying, Chen, Tunan, Chen, Ligang, Guo, Yuansen, Yang, Zhongbo, Mu, Ning, Feng, Hua, Zhang, Mingkun, Wang, Huabin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10040880/
https://www.ncbi.nlm.nih.gov/pubmed/36994356
http://dx.doi.org/10.3389/fbioe.2023.1143443
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author Fu, Ying
Chen, Tunan
Chen, Ligang
Guo, Yuansen
Yang, Zhongbo
Mu, Ning
Feng, Hua
Zhang, Mingkun
Wang, Huabin
author_facet Fu, Ying
Chen, Tunan
Chen, Ligang
Guo, Yuansen
Yang, Zhongbo
Mu, Ning
Feng, Hua
Zhang, Mingkun
Wang, Huabin
author_sort Fu, Ying
collection PubMed
description The integration of a microfluidic chip into terahertz time-domain attenuated total reflection (THz TD-ATR) spectroscopy is highly demanded for the accurate measurement of aqueous samples. Hitherto, however little work has been reported on this regard. Here, we demonstrate a strategy of fabricating a polydimethylsiloxane microfluidic chip (M-chip) suitable for the measurement of aqueous samples, and investigate the effects of its configuration, particularly the cavity depth of the M-chip on THz spectra. By measuring pure water, we find that the Fresnel formulae of two-interface model should be applied to analyze the THz spectral data when the depth is smaller than 210 μm, but the Fresnel formula of one-interface model can be applied when the depth is no less than 210 μm. We further validate this by measuring physiological solution and protein solution. This work can help promote the application of THz TD-ATR spectroscopy in the study of aqueous biological samples.
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spelling pubmed-100408802023-03-28 Terahertz time-domain attenuated total reflection spectroscopy integrated with a microfluidic chip Fu, Ying Chen, Tunan Chen, Ligang Guo, Yuansen Yang, Zhongbo Mu, Ning Feng, Hua Zhang, Mingkun Wang, Huabin Front Bioeng Biotechnol Bioengineering and Biotechnology The integration of a microfluidic chip into terahertz time-domain attenuated total reflection (THz TD-ATR) spectroscopy is highly demanded for the accurate measurement of aqueous samples. Hitherto, however little work has been reported on this regard. Here, we demonstrate a strategy of fabricating a polydimethylsiloxane microfluidic chip (M-chip) suitable for the measurement of aqueous samples, and investigate the effects of its configuration, particularly the cavity depth of the M-chip on THz spectra. By measuring pure water, we find that the Fresnel formulae of two-interface model should be applied to analyze the THz spectral data when the depth is smaller than 210 μm, but the Fresnel formula of one-interface model can be applied when the depth is no less than 210 μm. We further validate this by measuring physiological solution and protein solution. This work can help promote the application of THz TD-ATR spectroscopy in the study of aqueous biological samples. Frontiers Media S.A. 2023-03-13 /pmc/articles/PMC10040880/ /pubmed/36994356 http://dx.doi.org/10.3389/fbioe.2023.1143443 Text en Copyright © 2023 Fu, Chen, Chen, Guo, Yang, Mu, Feng, Zhang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Fu, Ying
Chen, Tunan
Chen, Ligang
Guo, Yuansen
Yang, Zhongbo
Mu, Ning
Feng, Hua
Zhang, Mingkun
Wang, Huabin
Terahertz time-domain attenuated total reflection spectroscopy integrated with a microfluidic chip
title Terahertz time-domain attenuated total reflection spectroscopy integrated with a microfluidic chip
title_full Terahertz time-domain attenuated total reflection spectroscopy integrated with a microfluidic chip
title_fullStr Terahertz time-domain attenuated total reflection spectroscopy integrated with a microfluidic chip
title_full_unstemmed Terahertz time-domain attenuated total reflection spectroscopy integrated with a microfluidic chip
title_short Terahertz time-domain attenuated total reflection spectroscopy integrated with a microfluidic chip
title_sort terahertz time-domain attenuated total reflection spectroscopy integrated with a microfluidic chip
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10040880/
https://www.ncbi.nlm.nih.gov/pubmed/36994356
http://dx.doi.org/10.3389/fbioe.2023.1143443
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