Cargando…

Monitoring the compaction of single DNA molecules in Xenopus egg extract in real time

DNA compaction is required for the condensation and resolution of chromosomes during mitosis, but the relative contribution of individual chromatin factors to this process is poorly understood. We developed a physiological, cell-free system using high-speed Xenopus egg extracts and optical tweezers...

Descripción completa

Detalles Bibliográficos
Autores principales:	Sun, Mingxuan, Amiri, Hossein, Tong, Alexander B., Shintomi, Keishi, Hirano, Tatsuya, Bustamante, Carlos, Heald, Rebecca
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	National Academy of Sciences 2023
Materias:	Biological Sciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10041109/ https://www.ncbi.nlm.nih.gov/pubmed/36917660 http://dx.doi.org/10.1073/pnas.2221309120

Ejemplares similares

Xenopus Egg Extracts Increase Dynamics of Histone H1 on Sperm Chromatin
por: Freedman, Benjamin S., et al.
Publicado: (2010)

Regulation of Op18 during Spindle Assembly in Xenopus Egg Extracts
por: Budde, Priya Prakash, et al.
Publicado: (2001)

TPX2 levels modulate meiotic spindle size and architecture in Xenopus egg extracts
por: Helmke, Kara J., et al.
Publicado: (2014)

Multiple Domains of Human CLASP Contribute to Microtubule Dynamics and Organization In Vitro and in Xenopus Egg Extracts
por: Patel, Kieren, et al.
Publicado: (2012)

Xenopus tropicalis egg extracts provide insight into scaling of the mitotic spindle
por: Brown, Katherine S., et al.
Publicado: (2007)

Histone H1 is essential for mitotic chromosome architecture and segregation in Xenopus laevis egg extracts
por: Maresca, Thomas J., et al.
Publicado: (2005)

Spindle Assembly in Xenopus Egg Extracts: Respective Roles of Centrosomes and Microtubule Self-Organization
por: Heald, Rebecca, et al.
Publicado: (1997)

The condensin complex is required for proper spindle assembly and chromosome segregation in Xenopus egg extracts
por: Wignall, Sarah M., et al.
Publicado: (2003)

Mitotic Spindle Assembly around RCC1-Coated Beads in Xenopus Egg Extracts
por: Halpin, David, et al.
Publicado: (2011)

Studying chromosome biology with single-molecule resolution in Xenopus laevis egg extracts
por: Cameron, George, et al.
Publicado: (2021)

Guiding functions of the C-terminal domain of topoisomerase IIα advance mitotic chromosome assembly
por: Shintomi, Keishi, et al.
Publicado: (2021)

Preparation and Fractionation of Xenopus laevis Egg Extracts
por: K. Cross, Marie, et al.
Publicado: (2008)

In vitro assembly of prenucleolar bodies in Xenopus egg extract
Publicado: (1992)

Microtubule assembly in cytoplasmic extracts of Xenopus oocytes and eggs
Publicado: (1987)

Peroxisome protein import recapitulated in Xenopus egg extracts
por: Romano, Fabian B., et al.
Publicado: (2019)

DNA damage-induced replication arrest in Xenopus egg extracts
por: Stokes, Matthew P., et al.
Publicado: (2003)

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
por: Cross, Marie, et al.
Publicado: (2008)

Evidence for an RNA Polymerization Activity in Axolotl and Xenopus Egg Extracts
por: Pelczar, Hélène, et al.
Publicado: (2010)

Non-homologous end joining repair in Xenopus egg extract
por: Zhu, Songli, et al.
Publicado: (2016)

The nuclear membrane determines the timing of DNA replication in Xenopus egg extracts
Publicado: (1991)

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
por: Sharp, Judith A., et al.
Publicado: (2013)

Affinity Purification of Label-free Tubulins from Xenopus Egg Extracts
por: Reusch, Sebastian, et al.
Publicado: (2020)

Xorbit/CLASP links dynamic microtubules to chromosomes in the Xenopus meiotic spindle
por: Hannak, Eva, et al.
Publicado: (2006)

Structural Transitions at Microtubule Ends Correlate with Their Dynamic Properties in Xenopus Egg Extracts
por: Arnal, Isabelle, et al.
Publicado: (2000)

Membrane-associated lamins in Xenopus egg extracts: identification of two vesicle populations
Publicado: (1993)

DNA replication is required for the checkpoint response to damaged DNA in Xenopus egg extracts
por: Stokes, Matthew P., et al.
Publicado: (2002)

DNA is a co-factor for its own replication in Xenopus egg extracts
por: Lebofsky, Ronald, et al.
Publicado: (2011)

Depletion of Uhrf1 inhibits chromosomal DNA replication in Xenopus egg extracts
por: Taylor, Elaine M., et al.
Publicado: (2013)

A computational model predicts Xenopus meiotic spindle organization
por: Loughlin, Rose, et al.
Publicado: (2010)

A versatile multivariate image analysis pipeline reveals features of Xenopus extract spindles
por: Grenfell, Andrew W., et al.
Publicado: (2016)

Xenopus laevis lectin is localized at several sites in Xenopus oocytes, eggs, and embryos
Publicado: (1983)

Parthenogenesis in Xenopus eggs requires centrosomal integrity
Publicado: (1990)

Obtaining Eggs from Xenopus laevis Females
por: K. Cross, Marie, et al.
Publicado: (2008)

Poleward transport of Eg5 by dynein–dynactin in Xenopus laevis egg extract spindles
por: Uteng, Marianne, et al.
Publicado: (2008)

Use of Xenopus laevis frog egg extract in diagnosing human male unexplained infertility.
por: Brown, D. B., et al.
Publicado: (1992)

Wee1-Regulated Apoptosis Mediated by the Crk Adaptor Protein in Xenopus Egg Extracts
por: Smith, Jesse J., et al.
Publicado: (2000)

Role of replication protein A as sensor in activation of the S-phase checkpoint in Xenopus egg extracts
por: Recolin, Bénédicte, et al.
Publicado: (2012)

MCM2-7 Form Double Hexamers at Licensed Origins in Xenopus Egg Extract
por: Gambus, Agnieszka, et al.
Publicado: (2011)

Preparation and use of Xenopus egg extracts to study DNA replication and chromatin associated proteins
por: Gillespie, Peter J., et al.
Publicado: (2012)

Autocatalytic microtubule nucleation determines the size and mass of Xenopus laevis egg extract spindles
por: Decker, Franziska, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_49ensu23d58b3a1oi2nul6krr0