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The role of NMDA glutamate receptors in lung injury caused by chronic long-term intermittent hypobaric hypoxia

Chronic intermittent hypoxia (CIH), a component of sleep apnea-hypopnea syndrome, is suggested to cause damage to lung tissue, and the role of glutamate is not well studied. We used a chronic long-term intermittent hypobaric hypoxia (CLTIHH) model of rats to find out if such procedure causes lung in...

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Autores principales: Yaman, M.O., Sönmez, O.F., Ekiz-Yilmaz, T., Sönmez, D., Meydanlı, E.E.G., Guner, I., Sahin, G., Dariyerli, N., Yelmen, N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10041672/
https://www.ncbi.nlm.nih.gov/pubmed/36995874
http://dx.doi.org/10.1590/1414-431X2023e12549
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author Yaman, M.O.
Sönmez, O.F.
Ekiz-Yilmaz, T.
Sönmez, D.
Meydanlı, E.E.G.
Guner, I.
Sahin, G.
Dariyerli, N.
Yelmen, N.
author_facet Yaman, M.O.
Sönmez, O.F.
Ekiz-Yilmaz, T.
Sönmez, D.
Meydanlı, E.E.G.
Guner, I.
Sahin, G.
Dariyerli, N.
Yelmen, N.
author_sort Yaman, M.O.
collection PubMed
description Chronic intermittent hypoxia (CIH), a component of sleep apnea-hypopnea syndrome, is suggested to cause damage to lung tissue, and the role of glutamate is not well studied. We used a chronic long-term intermittent hypobaric hypoxia (CLTIHH) model of rats to find out if such procedure causes lung injury and the potential effect of N-methyl-D-aspartate receptors (NMDARs) by using receptor antagonist MK-801 (dizocilpine). Thirty-two rats were placed into four groups; a control and three CLTIHH groups where rats were placed into a low-pressure chamber set to 430 mmHg for 5 h/day, 5 days/week, for 5 weeks. Only one group received MK-801 (0.3 mg/kg, ip) daily. We evaluated tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10, and nuclear factor (NF)-kB for the inflammatory process, superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPX), total antioxidant status (TAS), and total oxidant status (TOS) for oxidative stress, and caspase-9 levels. Blood plasma, bronchoalveolar fluid (BALF), and lung tissue extracts were evaluated. Both oxidant and inflammatory parameters were significantly increased in all the mediums of the CLTIHH groups except the group that received MK-801. Significant evidence was collected on MK-801 alleviating the effect of CLTIHH. Histological evaluations revealed lung damage and fibrotic changes in the CLTIHH groups. It was first shown that the CLTIHH procedure caused chronic lung injury, and that inflammation and oxidant stress were influential in the formation of lung injury. Secondly, NMDAR antagonist MK-801 effectively inhibited the development of lung injury and fibrosis.
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spelling pubmed-100416722023-03-28 The role of NMDA glutamate receptors in lung injury caused by chronic long-term intermittent hypobaric hypoxia Yaman, M.O. Sönmez, O.F. Ekiz-Yilmaz, T. Sönmez, D. Meydanlı, E.E.G. Guner, I. Sahin, G. Dariyerli, N. Yelmen, N. Braz J Med Biol Res Research Article Chronic intermittent hypoxia (CIH), a component of sleep apnea-hypopnea syndrome, is suggested to cause damage to lung tissue, and the role of glutamate is not well studied. We used a chronic long-term intermittent hypobaric hypoxia (CLTIHH) model of rats to find out if such procedure causes lung injury and the potential effect of N-methyl-D-aspartate receptors (NMDARs) by using receptor antagonist MK-801 (dizocilpine). Thirty-two rats were placed into four groups; a control and three CLTIHH groups where rats were placed into a low-pressure chamber set to 430 mmHg for 5 h/day, 5 days/week, for 5 weeks. Only one group received MK-801 (0.3 mg/kg, ip) daily. We evaluated tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10, and nuclear factor (NF)-kB for the inflammatory process, superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPX), total antioxidant status (TAS), and total oxidant status (TOS) for oxidative stress, and caspase-9 levels. Blood plasma, bronchoalveolar fluid (BALF), and lung tissue extracts were evaluated. Both oxidant and inflammatory parameters were significantly increased in all the mediums of the CLTIHH groups except the group that received MK-801. Significant evidence was collected on MK-801 alleviating the effect of CLTIHH. Histological evaluations revealed lung damage and fibrotic changes in the CLTIHH groups. It was first shown that the CLTIHH procedure caused chronic lung injury, and that inflammation and oxidant stress were influential in the formation of lung injury. Secondly, NMDAR antagonist MK-801 effectively inhibited the development of lung injury and fibrosis. Associação Brasileira de Divulgação Científica 2023-03-24 /pmc/articles/PMC10041672/ /pubmed/36995874 http://dx.doi.org/10.1590/1414-431X2023e12549 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yaman, M.O.
Sönmez, O.F.
Ekiz-Yilmaz, T.
Sönmez, D.
Meydanlı, E.E.G.
Guner, I.
Sahin, G.
Dariyerli, N.
Yelmen, N.
The role of NMDA glutamate receptors in lung injury caused by chronic long-term intermittent hypobaric hypoxia
title The role of NMDA glutamate receptors in lung injury caused by chronic long-term intermittent hypobaric hypoxia
title_full The role of NMDA glutamate receptors in lung injury caused by chronic long-term intermittent hypobaric hypoxia
title_fullStr The role of NMDA glutamate receptors in lung injury caused by chronic long-term intermittent hypobaric hypoxia
title_full_unstemmed The role of NMDA glutamate receptors in lung injury caused by chronic long-term intermittent hypobaric hypoxia
title_short The role of NMDA glutamate receptors in lung injury caused by chronic long-term intermittent hypobaric hypoxia
title_sort role of nmda glutamate receptors in lung injury caused by chronic long-term intermittent hypobaric hypoxia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10041672/
https://www.ncbi.nlm.nih.gov/pubmed/36995874
http://dx.doi.org/10.1590/1414-431X2023e12549
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