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Antimicrobial efficacy and inactivation kinetics of a novel LED-based UV-irradiation technology

BACKGROUND: Ultraviolet (UV)-light-emitting diodes (UV-LEDs) are energy efficient and of special interest for the inactivation of micro-organisms. In the context of the coronavirus disease 2019 pandemic, novel UV technologies can offer a powerful alternative for effective infection prevention and co...

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Autores principales: Schöbel, H., Diem, G., Kiechl, J., Chistè, D., Bertacchi, G., Mayr, A., Wilflingseder, D., Lass-Flörl, C., Posch, W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Ltd on behalf of The Healthcare Infection Society. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10041887/
https://www.ncbi.nlm.nih.gov/pubmed/36754288
http://dx.doi.org/10.1016/j.jhin.2022.12.023
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author Schöbel, H.
Diem, G.
Kiechl, J.
Chistè, D.
Bertacchi, G.
Mayr, A.
Wilflingseder, D.
Lass-Flörl, C.
Posch, W.
author_facet Schöbel, H.
Diem, G.
Kiechl, J.
Chistè, D.
Bertacchi, G.
Mayr, A.
Wilflingseder, D.
Lass-Flörl, C.
Posch, W.
author_sort Schöbel, H.
collection PubMed
description BACKGROUND: Ultraviolet (UV)-light-emitting diodes (UV-LEDs) are energy efficient and of special interest for the inactivation of micro-organisms. In the context of the coronavirus disease 2019 pandemic, novel UV technologies can offer a powerful alternative for effective infection prevention and control. METHODS: This study assessed the antimicrobial efficacy of UV-C LEDs on Escherichia coli, Pseudomonas fluorescens and Listeria innocua, as well as severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), human immunodeficiency virus-1 (HIV-1) and murine norovirus (MNV), dried on inanimate surfaces, based on European Standard EN 17272. RESULTS: This study found 90% inactivation rates for the tested bacteria at mean UV-C doses, averaged over all three investigated UV-C wavelengths, of 1.7 mJ/cm(2) for E. coli, 1.9 mJ/cm(2) for P. fluorescens and 1.5 mJ/cm(2) for L. innocua. For the tested viruses, UV doses <15 mJ/cm(2) resulted in 90% inactivation at wavelengths of 255 and 265 nm. Exposure of viruses to longer UV wavelengths, such as 275 and 285 nm, required much higher doses (up to 120 mJ/cm(2)) for inactivation. Regarding inactivation, non-enveloped MNV required much higher UV doses for all tested wavelengths compared with SARS-CoV-2 or HIV-1. CONCLUSION: Overall, the results support the use of LEDs emitting at shorter wavelengths of the UV-C spectrum to inactivate bacteria as well as enveloped and non-enveloped viruses by exposure to the appropriate UV dose. However, low availability and excessive production costs of shortwave UV-C LEDs restricts implementation at present, and supports the use of longwave UV-C LEDs in combination with higher irradiation doses.
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spelling pubmed-100418872023-03-27 Antimicrobial efficacy and inactivation kinetics of a novel LED-based UV-irradiation technology Schöbel, H. Diem, G. Kiechl, J. Chistè, D. Bertacchi, G. Mayr, A. Wilflingseder, D. Lass-Flörl, C. Posch, W. J Hosp Infect Article BACKGROUND: Ultraviolet (UV)-light-emitting diodes (UV-LEDs) are energy efficient and of special interest for the inactivation of micro-organisms. In the context of the coronavirus disease 2019 pandemic, novel UV technologies can offer a powerful alternative for effective infection prevention and control. METHODS: This study assessed the antimicrobial efficacy of UV-C LEDs on Escherichia coli, Pseudomonas fluorescens and Listeria innocua, as well as severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), human immunodeficiency virus-1 (HIV-1) and murine norovirus (MNV), dried on inanimate surfaces, based on European Standard EN 17272. RESULTS: This study found 90% inactivation rates for the tested bacteria at mean UV-C doses, averaged over all three investigated UV-C wavelengths, of 1.7 mJ/cm(2) for E. coli, 1.9 mJ/cm(2) for P. fluorescens and 1.5 mJ/cm(2) for L. innocua. For the tested viruses, UV doses <15 mJ/cm(2) resulted in 90% inactivation at wavelengths of 255 and 265 nm. Exposure of viruses to longer UV wavelengths, such as 275 and 285 nm, required much higher doses (up to 120 mJ/cm(2)) for inactivation. Regarding inactivation, non-enveloped MNV required much higher UV doses for all tested wavelengths compared with SARS-CoV-2 or HIV-1. CONCLUSION: Overall, the results support the use of LEDs emitting at shorter wavelengths of the UV-C spectrum to inactivate bacteria as well as enveloped and non-enveloped viruses by exposure to the appropriate UV dose. However, low availability and excessive production costs of shortwave UV-C LEDs restricts implementation at present, and supports the use of longwave UV-C LEDs in combination with higher irradiation doses. The Authors. Published by Elsevier Ltd on behalf of The Healthcare Infection Society. 2023-05 2023-02-06 /pmc/articles/PMC10041887/ /pubmed/36754288 http://dx.doi.org/10.1016/j.jhin.2022.12.023 Text en © 2023 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Schöbel, H.
Diem, G.
Kiechl, J.
Chistè, D.
Bertacchi, G.
Mayr, A.
Wilflingseder, D.
Lass-Flörl, C.
Posch, W.
Antimicrobial efficacy and inactivation kinetics of a novel LED-based UV-irradiation technology
title Antimicrobial efficacy and inactivation kinetics of a novel LED-based UV-irradiation technology
title_full Antimicrobial efficacy and inactivation kinetics of a novel LED-based UV-irradiation technology
title_fullStr Antimicrobial efficacy and inactivation kinetics of a novel LED-based UV-irradiation technology
title_full_unstemmed Antimicrobial efficacy and inactivation kinetics of a novel LED-based UV-irradiation technology
title_short Antimicrobial efficacy and inactivation kinetics of a novel LED-based UV-irradiation technology
title_sort antimicrobial efficacy and inactivation kinetics of a novel led-based uv-irradiation technology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10041887/
https://www.ncbi.nlm.nih.gov/pubmed/36754288
http://dx.doi.org/10.1016/j.jhin.2022.12.023
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