Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study

BACKGROUND: This study aimed to describe the kinetics of Leishmania parasite load determined using kinetoplast DNA (kDNA)-based quantitative polymerase chain reaction (qPCR) in visceral leishmaniasis (VL) patients. METHODS: Parasite load in blood was assessed by qPCR at five time points, up to 12 mo...

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Detalles Bibliográficos
Autores principales: de Aquino, Samuel Ricarte, Diniz, Lucyo Flávio Bezerra, Nunes, Sávio Luiz Pereira, Silva, Roberta Lane de Oliveira, Gouveia, Gisele Veneroni, Gouveia, João José Simoni, Sales, Kamila Gaudêncio da Silva, Dantas-Torres, Filipe, do Carmo, Rodrigo Feliciano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10042474/
https://www.ncbi.nlm.nih.gov/pubmed/36995785
http://dx.doi.org/10.1590/0037-8682-0456-2022
Descripción
Sumario:BACKGROUND: This study aimed to describe the kinetics of Leishmania parasite load determined using kinetoplast DNA (kDNA)-based quantitative polymerase chain reaction (qPCR) in visceral leishmaniasis (VL) patients. METHODS: Parasite load in blood was assessed by qPCR at five time points, up to 12 months post-diagnosis. Sixteen patients were followed up. RESULTS: A significant reduction in the parasite load was observed after treatment (P < 0.0001). One patient had an increased parasite load 3 months post-treatment and relapsed clinically at month six. CONCLUSIONS: We have described the use of kDNA-based qPCR in the post-treatment follow-up of VL cases.

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