Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study

BACKGROUND: This study aimed to describe the kinetics of Leishmania parasite load determined using kinetoplast DNA (kDNA)-based quantitative polymerase chain reaction (qPCR) in visceral leishmaniasis (VL) patients. METHODS: Parasite load in blood was assessed by qPCR at five time points, up to 12 mo...

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Autores principales: de Aquino, Samuel Ricarte, Diniz, Lucyo Flávio Bezerra, Nunes, Sávio Luiz Pereira, Silva, Roberta Lane de Oliveira, Gouveia, Gisele Veneroni, Gouveia, João José Simoni, Sales, Kamila Gaudêncio da Silva, Dantas-Torres, Filipe, do Carmo, Rodrigo Feliciano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10042474/
https://www.ncbi.nlm.nih.gov/pubmed/36995785
http://dx.doi.org/10.1590/0037-8682-0456-2022
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author de Aquino, Samuel Ricarte
Diniz, Lucyo Flávio Bezerra
Nunes, Sávio Luiz Pereira
Silva, Roberta Lane de Oliveira
Gouveia, Gisele Veneroni
Gouveia, João José Simoni
Sales, Kamila Gaudêncio da Silva
Dantas-Torres, Filipe
do Carmo, Rodrigo Feliciano
author_facet de Aquino, Samuel Ricarte
Diniz, Lucyo Flávio Bezerra
Nunes, Sávio Luiz Pereira
Silva, Roberta Lane de Oliveira
Gouveia, Gisele Veneroni
Gouveia, João José Simoni
Sales, Kamila Gaudêncio da Silva
Dantas-Torres, Filipe
do Carmo, Rodrigo Feliciano
author_sort de Aquino, Samuel Ricarte
collection PubMed
description BACKGROUND: This study aimed to describe the kinetics of Leishmania parasite load determined using kinetoplast DNA (kDNA)-based quantitative polymerase chain reaction (qPCR) in visceral leishmaniasis (VL) patients. METHODS: Parasite load in blood was assessed by qPCR at five time points, up to 12 months post-diagnosis. Sixteen patients were followed up. RESULTS: A significant reduction in the parasite load was observed after treatment (P < 0.0001). One patient had an increased parasite load 3 months post-treatment and relapsed clinically at month six. CONCLUSIONS: We have described the use of kDNA-based qPCR in the post-treatment follow-up of VL cases.
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spelling pubmed-100424742023-03-28 Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study de Aquino, Samuel Ricarte Diniz, Lucyo Flávio Bezerra Nunes, Sávio Luiz Pereira Silva, Roberta Lane de Oliveira Gouveia, Gisele Veneroni Gouveia, João José Simoni Sales, Kamila Gaudêncio da Silva Dantas-Torres, Filipe do Carmo, Rodrigo Feliciano Rev Soc Bras Med Trop Short Communication BACKGROUND: This study aimed to describe the kinetics of Leishmania parasite load determined using kinetoplast DNA (kDNA)-based quantitative polymerase chain reaction (qPCR) in visceral leishmaniasis (VL) patients. METHODS: Parasite load in blood was assessed by qPCR at five time points, up to 12 months post-diagnosis. Sixteen patients were followed up. RESULTS: A significant reduction in the parasite load was observed after treatment (P < 0.0001). One patient had an increased parasite load 3 months post-treatment and relapsed clinically at month six. CONCLUSIONS: We have described the use of kDNA-based qPCR in the post-treatment follow-up of VL cases. Sociedade Brasileira de Medicina Tropical - SBMT 2023-03-27 /pmc/articles/PMC10042474/ /pubmed/36995785 http://dx.doi.org/10.1590/0037-8682-0456-2022 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Short Communication
de Aquino, Samuel Ricarte
Diniz, Lucyo Flávio Bezerra
Nunes, Sávio Luiz Pereira
Silva, Roberta Lane de Oliveira
Gouveia, Gisele Veneroni
Gouveia, João José Simoni
Sales, Kamila Gaudêncio da Silva
Dantas-Torres, Filipe
do Carmo, Rodrigo Feliciano
Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study
title Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study
title_full Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study
title_fullStr Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study
title_full_unstemmed Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study
title_short Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study
title_sort blood parasite load by qpcr as therapeutic monitoring in visceral leishmaniasis patients in brazil: a case series study
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10042474/
https://www.ncbi.nlm.nih.gov/pubmed/36995785
http://dx.doi.org/10.1590/0037-8682-0456-2022
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