Cargando…
Transcriptomic analysis reveals the mechanism of the alleviation of salt stress by salicylic acid in pepper (Capsicum annuum L.)
BACKGROUND: The growth and yield of pepper (Capsicum annuum L.) is often affected by the critical salt stress. Salicylic acid (SA) can improve plants’ stress tolerance by promoting growth and regulating ion absorption and transportation. METHODS AND RESULTS: To uncover the alleviated mechanism of sa...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10042771/ https://www.ncbi.nlm.nih.gov/pubmed/36418774 http://dx.doi.org/10.1007/s11033-022-08064-y |
_version_ | 1784913003609587712 |
---|---|
author | Ma, Jing Wang, Ying Wang, Li-yue Lin, Duo Yang, Yanjie |
author_facet | Ma, Jing Wang, Ying Wang, Li-yue Lin, Duo Yang, Yanjie |
author_sort | Ma, Jing |
collection | PubMed |
description | BACKGROUND: The growth and yield of pepper (Capsicum annuum L.) is often affected by the critical salt stress. Salicylic acid (SA) can improve plants’ stress tolerance by promoting growth and regulating ion absorption and transportation. METHODS AND RESULTS: To uncover the alleviated mechanism of salt stress by SA in pepper, we conducted morphological, physiological, cytological, and transcriptomic analyses under a single SA treatment and NaCl with and without SA pre-treatment for 9 days. Seedlings under NaCl treatment showed yellow shrunken leaves, this tatus were alleviated by NS treatment (NaCl with SA pre-treatment). Compared with plants under NaCl treatment, those in the NS treatment showed reduced lipid peroxidation, and significantly increased contents of chlorophyll and osmotic regulators (proline, soluble sugars). Treatment with SA balanced the Na(+)/K(+) ratio. We conducted transcriptome sequencing and identified differentially expressed genes (DEGs) contributing to alleviation of salt stress by SA in pepper. Besides photosynthesis related genes, GO and KEGG analyses revealed that the DEGs were enriched in ‘sequence-specific DNA binding’, ‘transcription regulator activity’ and ‘DNA binding transcription factor activity’ by GO terms. And our results showed that TFs, such as MYB, bZIP, BBX, AP2/ERF, NAC, etc., probably make a great contribution in the alleviation of salt stress by SA. CONCLUSIONS: These results reveal that SA can improve plants’ stress tolerance by balancing ion absorption, gene expression and transcriptional regulation, which provide new ideas and resources for subsequent research on the mechanism of salt tolerance in pepper. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11033-022-08064-y. |
format | Online Article Text |
id | pubmed-10042771 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-100427712023-03-29 Transcriptomic analysis reveals the mechanism of the alleviation of salt stress by salicylic acid in pepper (Capsicum annuum L.) Ma, Jing Wang, Ying Wang, Li-yue Lin, Duo Yang, Yanjie Mol Biol Rep Original Article BACKGROUND: The growth and yield of pepper (Capsicum annuum L.) is often affected by the critical salt stress. Salicylic acid (SA) can improve plants’ stress tolerance by promoting growth and regulating ion absorption and transportation. METHODS AND RESULTS: To uncover the alleviated mechanism of salt stress by SA in pepper, we conducted morphological, physiological, cytological, and transcriptomic analyses under a single SA treatment and NaCl with and without SA pre-treatment for 9 days. Seedlings under NaCl treatment showed yellow shrunken leaves, this tatus were alleviated by NS treatment (NaCl with SA pre-treatment). Compared with plants under NaCl treatment, those in the NS treatment showed reduced lipid peroxidation, and significantly increased contents of chlorophyll and osmotic regulators (proline, soluble sugars). Treatment with SA balanced the Na(+)/K(+) ratio. We conducted transcriptome sequencing and identified differentially expressed genes (DEGs) contributing to alleviation of salt stress by SA in pepper. Besides photosynthesis related genes, GO and KEGG analyses revealed that the DEGs were enriched in ‘sequence-specific DNA binding’, ‘transcription regulator activity’ and ‘DNA binding transcription factor activity’ by GO terms. And our results showed that TFs, such as MYB, bZIP, BBX, AP2/ERF, NAC, etc., probably make a great contribution in the alleviation of salt stress by SA. CONCLUSIONS: These results reveal that SA can improve plants’ stress tolerance by balancing ion absorption, gene expression and transcriptional regulation, which provide new ideas and resources for subsequent research on the mechanism of salt tolerance in pepper. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11033-022-08064-y. Springer Netherlands 2022-11-23 2023 /pmc/articles/PMC10042771/ /pubmed/36418774 http://dx.doi.org/10.1007/s11033-022-08064-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Ma, Jing Wang, Ying Wang, Li-yue Lin, Duo Yang, Yanjie Transcriptomic analysis reveals the mechanism of the alleviation of salt stress by salicylic acid in pepper (Capsicum annuum L.) |
title | Transcriptomic analysis reveals the mechanism of the alleviation of salt stress by salicylic acid in pepper (Capsicum annuum L.) |
title_full | Transcriptomic analysis reveals the mechanism of the alleviation of salt stress by salicylic acid in pepper (Capsicum annuum L.) |
title_fullStr | Transcriptomic analysis reveals the mechanism of the alleviation of salt stress by salicylic acid in pepper (Capsicum annuum L.) |
title_full_unstemmed | Transcriptomic analysis reveals the mechanism of the alleviation of salt stress by salicylic acid in pepper (Capsicum annuum L.) |
title_short | Transcriptomic analysis reveals the mechanism of the alleviation of salt stress by salicylic acid in pepper (Capsicum annuum L.) |
title_sort | transcriptomic analysis reveals the mechanism of the alleviation of salt stress by salicylic acid in pepper (capsicum annuum l.) |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10042771/ https://www.ncbi.nlm.nih.gov/pubmed/36418774 http://dx.doi.org/10.1007/s11033-022-08064-y |
work_keys_str_mv | AT majing transcriptomicanalysisrevealsthemechanismofthealleviationofsaltstressbysalicylicacidinpeppercapsicumannuuml AT wangying transcriptomicanalysisrevealsthemechanismofthealleviationofsaltstressbysalicylicacidinpeppercapsicumannuuml AT wangliyue transcriptomicanalysisrevealsthemechanismofthealleviationofsaltstressbysalicylicacidinpeppercapsicumannuuml AT linduo transcriptomicanalysisrevealsthemechanismofthealleviationofsaltstressbysalicylicacidinpeppercapsicumannuuml AT yangyanjie transcriptomicanalysisrevealsthemechanismofthealleviationofsaltstressbysalicylicacidinpeppercapsicumannuuml |