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Optimization of long-range PCR protocol to prepare filaggrin exon 3 libraries for PacBio long-read sequencing

BACKGROUND: The filaggrin (FLG) protein, encoded by the FLG gene, is an intermediate filament-associated protein that plays a crucial role in the terminal stages of human epidermal differentiation. Loss-of-function mutations in the FLG exon 3 have been associated with skin diseases. The identificati...

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Detalles Bibliográficos
Autores principales:	Mareso, Chiara, Albion, Elena, Cozza, William, Tanzi, Benedetta, Cecchin, Stefano, Gisondi, Paolo, Michelini, Sandro, Bellinato, Francesco, Michelini, Serena, Michelini, Silvia, Bertelli, Matteo, Marceddu, Giuseppe
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Netherlands 2023
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10042914/ https://www.ncbi.nlm.nih.gov/pubmed/36692677 http://dx.doi.org/10.1007/s11033-022-08170-x

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