Incubation of Lymphocytes with IL-2 Causes the Appearance of Antitumor T Cells Carrying FC, CD25, and LFA-1 on the Surface

To carry out antitumor activity against cells that have lost surface antigens, human lymphocytes must have a certain repertoire of surface proteins capable of contacting a tumor cell and inducing programmed cell death in it. In this work, we showed that activation of healthy donor cells by IL-2 cyto...

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Autores principales: Ivanova, O. K., Sharapova, T. N., Romanova, E. A., Yashin, D. V., Sashchenko, L. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2023
Materias:
Biochemistry, Biophysics, and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10042969/
https://www.ncbi.nlm.nih.gov/pubmed/36653582
http://dx.doi.org/10.1134/S160767292270003X
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author Ivanova, O. K.
Sharapova, T. N.
Romanova, E. A.
Yashin, D. V.
Sashchenko, L. P.
author_facet Ivanova, O. K.
Sharapova, T. N.
Romanova, E. A.
Yashin, D. V.
Sashchenko, L. P.
author_sort Ivanova, O. K.
collection PubMed
description To carry out antitumor activity against cells that have lost surface antigens, human lymphocytes must have a certain repertoire of surface proteins capable of contacting a tumor cell and inducing programmed cell death in it. In this work, we showed that activation of healthy donor cells by IL-2 cytokine within 6 days causes the appearance of FasL, CD25, and LFA-1 proteins on CD8+CD25+ T lymphocytes, and also converts the LFA-1 protein into an active form having a high affinity for its target, ICAM-1 integrin. The appearance of these proteins on the surface of this subpopulation of lymphocytes allows them to induce programmed cell death in HLA-negative tumor cells.
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spelling pubmed-100429692023-03-29 Incubation of Lymphocytes with IL-2 Causes the Appearance of Antitumor T Cells Carrying FC, CD25, and LFA-1 on the Surface Ivanova, O. K. Sharapova, T. N. Romanova, E. A. Yashin, D. V. Sashchenko, L. P. Dokl Biochem Biophys Biochemistry, Biophysics, and Molecular Biology To carry out antitumor activity against cells that have lost surface antigens, human lymphocytes must have a certain repertoire of surface proteins capable of contacting a tumor cell and inducing programmed cell death in it. In this work, we showed that activation of healthy donor cells by IL-2 cytokine within 6 days causes the appearance of FasL, CD25, and LFA-1 proteins on CD8+CD25+ T lymphocytes, and also converts the LFA-1 protein into an active form having a high affinity for its target, ICAM-1 integrin. The appearance of these proteins on the surface of this subpopulation of lymphocytes allows them to induce programmed cell death in HLA-negative tumor cells. Pleiades Publishing 2023-01-18 2023 /pmc/articles/PMC10042969/ /pubmed/36653582 http://dx.doi.org/10.1134/S160767292270003X Text en © The Author(s) 2023, ISSN 1607-6729, Doklady Biochemistry and Biophysics, 2023, Vol. 508, pp. 12–16. © The Author(s), 2023. This article is an open access publication.ISSN 1607-6729, Doklady Biochemistry and Biophysics, 2022. © The Author(s), 2022. This article is an open access publication. https://creativecommons.org/licenses/by/4.0/Open Access.This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biochemistry, Biophysics, and Molecular Biology
Ivanova, O. K.
Sharapova, T. N.
Romanova, E. A.
Yashin, D. V.
Sashchenko, L. P.
Incubation of Lymphocytes with IL-2 Causes the Appearance of Antitumor T Cells Carrying FC, CD25, and LFA-1 on the Surface
title Incubation of Lymphocytes with IL-2 Causes the Appearance of Antitumor T Cells Carrying FC, CD25, and LFA-1 on the Surface
title_full Incubation of Lymphocytes with IL-2 Causes the Appearance of Antitumor T Cells Carrying FC, CD25, and LFA-1 on the Surface
title_fullStr Incubation of Lymphocytes with IL-2 Causes the Appearance of Antitumor T Cells Carrying FC, CD25, and LFA-1 on the Surface
title_full_unstemmed Incubation of Lymphocytes with IL-2 Causes the Appearance of Antitumor T Cells Carrying FC, CD25, and LFA-1 on the Surface
title_short Incubation of Lymphocytes with IL-2 Causes the Appearance of Antitumor T Cells Carrying FC, CD25, and LFA-1 on the Surface
title_sort incubation of lymphocytes with il-2 causes the appearance of antitumor t cells carrying fc, cd25, and lfa-1 on the surface
topic Biochemistry, Biophysics, and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10042969/
https://www.ncbi.nlm.nih.gov/pubmed/36653582
http://dx.doi.org/10.1134/S160767292270003X
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