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Genome, biology and stability of the Thurquoise phage – A new virus from the Bastillevirinae subfamily

Bacteriophages from the Bastillevirinae subfamily (Herelleviridae family) have proven to be effective against bacteria from the Bacillus genus including organisms from the B. cereus group, which cause food poisoning and persistent contamination of industrial installations. However, successful applic...

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Autores principales: Węglewska, Martyna, Barylski, Jakub, Wojnarowski, Filip, Nowicki, Grzegorz, Łukaszewicz, Marcin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043171/
https://www.ncbi.nlm.nih.gov/pubmed/36998400
http://dx.doi.org/10.3389/fmicb.2023.1120147
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author Węglewska, Martyna
Barylski, Jakub
Wojnarowski, Filip
Nowicki, Grzegorz
Łukaszewicz, Marcin
author_facet Węglewska, Martyna
Barylski, Jakub
Wojnarowski, Filip
Nowicki, Grzegorz
Łukaszewicz, Marcin
author_sort Węglewska, Martyna
collection PubMed
description Bacteriophages from the Bastillevirinae subfamily (Herelleviridae family) have proven to be effective against bacteria from the Bacillus genus including organisms from the B. cereus group, which cause food poisoning and persistent contamination of industrial installations. However, successful application of these phages in biocontrol depends on understanding of their biology and stability in different environments. In this study, we isolated a novel virus from garden soil in Wrocław (Poland) and named it ‘Thurquoise’. The genome of that phage was sequenced and assembled into a single continuous contig with 226 predicted protein-coding genes and 18 tRNAs. The cryo-electron microscopy revealed that Thurquoise has complex virion structure typical for the Bastillevirinae family. Confirmed hosts include selected bacteria from the Bacillus cereus group–specifically B. thuringiensis (isolation host) and B. mycoides, but susceptible strains display different efficiency of plating (EOP). The eclipse and latent periods of Thurquoise in the isolation host last ~ 50 min and ~ 70 min, respectively. The phage remains viable for more than 8 weeks in variants of the SM buffer with magnesium, calcium, caesium, manganese or potassium and can withstand numerous freeze–thaw cycles if protected by the addition of 15% glycerol or, to a lesser extent, 2% gelatine. Thus, with proper buffer formulation, this virus can be safely stored in common freezers and refrigerators for a considerable time. The Thurquoise phage is the exemplar of a new candidate species within the Caeruleovirus genus in the Bastillevirinae subfamily of the Herelleviridae family with a genome, morphology and biology typical for these taxa.
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spelling pubmed-100431712023-03-29 Genome, biology and stability of the Thurquoise phage – A new virus from the Bastillevirinae subfamily Węglewska, Martyna Barylski, Jakub Wojnarowski, Filip Nowicki, Grzegorz Łukaszewicz, Marcin Front Microbiol Microbiology Bacteriophages from the Bastillevirinae subfamily (Herelleviridae family) have proven to be effective against bacteria from the Bacillus genus including organisms from the B. cereus group, which cause food poisoning and persistent contamination of industrial installations. However, successful application of these phages in biocontrol depends on understanding of their biology and stability in different environments. In this study, we isolated a novel virus from garden soil in Wrocław (Poland) and named it ‘Thurquoise’. The genome of that phage was sequenced and assembled into a single continuous contig with 226 predicted protein-coding genes and 18 tRNAs. The cryo-electron microscopy revealed that Thurquoise has complex virion structure typical for the Bastillevirinae family. Confirmed hosts include selected bacteria from the Bacillus cereus group–specifically B. thuringiensis (isolation host) and B. mycoides, but susceptible strains display different efficiency of plating (EOP). The eclipse and latent periods of Thurquoise in the isolation host last ~ 50 min and ~ 70 min, respectively. The phage remains viable for more than 8 weeks in variants of the SM buffer with magnesium, calcium, caesium, manganese or potassium and can withstand numerous freeze–thaw cycles if protected by the addition of 15% glycerol or, to a lesser extent, 2% gelatine. Thus, with proper buffer formulation, this virus can be safely stored in common freezers and refrigerators for a considerable time. The Thurquoise phage is the exemplar of a new candidate species within the Caeruleovirus genus in the Bastillevirinae subfamily of the Herelleviridae family with a genome, morphology and biology typical for these taxa. Frontiers Media S.A. 2023-03-14 /pmc/articles/PMC10043171/ /pubmed/36998400 http://dx.doi.org/10.3389/fmicb.2023.1120147 Text en Copyright © 2023 Węglewska, Barylski, Wojnarowski, Nowicki and Łukaszewicz. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Węglewska, Martyna
Barylski, Jakub
Wojnarowski, Filip
Nowicki, Grzegorz
Łukaszewicz, Marcin
Genome, biology and stability of the Thurquoise phage – A new virus from the Bastillevirinae subfamily
title Genome, biology and stability of the Thurquoise phage – A new virus from the Bastillevirinae subfamily
title_full Genome, biology and stability of the Thurquoise phage – A new virus from the Bastillevirinae subfamily
title_fullStr Genome, biology and stability of the Thurquoise phage – A new virus from the Bastillevirinae subfamily
title_full_unstemmed Genome, biology and stability of the Thurquoise phage – A new virus from the Bastillevirinae subfamily
title_short Genome, biology and stability of the Thurquoise phage – A new virus from the Bastillevirinae subfamily
title_sort genome, biology and stability of the thurquoise phage – a new virus from the bastillevirinae subfamily
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043171/
https://www.ncbi.nlm.nih.gov/pubmed/36998400
http://dx.doi.org/10.3389/fmicb.2023.1120147
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