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CRISPR/Cas9-mediated mutagenesis of the susceptibility gene OsHPP04 in rice confers enhanced resistance to rice root-knot nematode

CRISPR crops carrying a mutation in susceptibility (S) genes provide an effective strategy for controlling plant disease, because they could be ‘transgene-free’ and commonly have more broad-spectrum and durable type of resistance. Despite their importance, CRISPR/Cas9-mediated editing of S genes for...

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Autores principales: Huang, Qiuling, Lin, Borong, Cao, Yuqing, Zhang, Yu, Song, Handa, Huang, Chunhui, Sun, Tianling, Long, Changwen, Liao, Jinling, Zhuo, Kan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043372/
https://www.ncbi.nlm.nih.gov/pubmed/36998699
http://dx.doi.org/10.3389/fpls.2023.1134653
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author Huang, Qiuling
Lin, Borong
Cao, Yuqing
Zhang, Yu
Song, Handa
Huang, Chunhui
Sun, Tianling
Long, Changwen
Liao, Jinling
Zhuo, Kan
author_facet Huang, Qiuling
Lin, Borong
Cao, Yuqing
Zhang, Yu
Song, Handa
Huang, Chunhui
Sun, Tianling
Long, Changwen
Liao, Jinling
Zhuo, Kan
author_sort Huang, Qiuling
collection PubMed
description CRISPR crops carrying a mutation in susceptibility (S) genes provide an effective strategy for controlling plant disease, because they could be ‘transgene-free’ and commonly have more broad-spectrum and durable type of resistance. Despite their importance, CRISPR/Cas9-mediated editing of S genes for engineering resistance to plant-parasitic nematode (PPN) disease has not been reported. In this study, we employed the CRISPR/Cas9 system to specifically induce targeted mutagenesis of the S gene rice copper metallochaperone heavy metal-associated plant protein 04 (OsHPP04), and successfully obtained genetically stable homozygous rice mutants with or without transgenic elements. These mutants confer enhanced resistance to the rice root-knot nematode (Meloidogyne graminicola), a major plant pathogenic nematode in rice agriculture. Moreover, the plant immune responses triggered by flg22, including reactive oxygen species burst, defence-related genes expression and callose deposition, were enhanced in the ‘transgene-free’ homozygous mutants. Analysis of rice growth and agronomic traits of two independent mutants showed that there are no obvious differences between wild-type plants and mutants. These findings suggest that OsHPP04 may be an S gene as a negative regulator of host immunity and genetic modification of S genes through the CRISPR/Cas9 technology can be used as a powerful tool to generate PPN resistant plant varieties.
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spelling pubmed-100433722023-03-29 CRISPR/Cas9-mediated mutagenesis of the susceptibility gene OsHPP04 in rice confers enhanced resistance to rice root-knot nematode Huang, Qiuling Lin, Borong Cao, Yuqing Zhang, Yu Song, Handa Huang, Chunhui Sun, Tianling Long, Changwen Liao, Jinling Zhuo, Kan Front Plant Sci Plant Science CRISPR crops carrying a mutation in susceptibility (S) genes provide an effective strategy for controlling plant disease, because they could be ‘transgene-free’ and commonly have more broad-spectrum and durable type of resistance. Despite their importance, CRISPR/Cas9-mediated editing of S genes for engineering resistance to plant-parasitic nematode (PPN) disease has not been reported. In this study, we employed the CRISPR/Cas9 system to specifically induce targeted mutagenesis of the S gene rice copper metallochaperone heavy metal-associated plant protein 04 (OsHPP04), and successfully obtained genetically stable homozygous rice mutants with or without transgenic elements. These mutants confer enhanced resistance to the rice root-knot nematode (Meloidogyne graminicola), a major plant pathogenic nematode in rice agriculture. Moreover, the plant immune responses triggered by flg22, including reactive oxygen species burst, defence-related genes expression and callose deposition, were enhanced in the ‘transgene-free’ homozygous mutants. Analysis of rice growth and agronomic traits of two independent mutants showed that there are no obvious differences between wild-type plants and mutants. These findings suggest that OsHPP04 may be an S gene as a negative regulator of host immunity and genetic modification of S genes through the CRISPR/Cas9 technology can be used as a powerful tool to generate PPN resistant plant varieties. Frontiers Media S.A. 2023-03-14 /pmc/articles/PMC10043372/ /pubmed/36998699 http://dx.doi.org/10.3389/fpls.2023.1134653 Text en Copyright © 2023 Huang, Lin, Cao, Zhang, Song, Huang, Sun, Long, Liao and Zhuo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Huang, Qiuling
Lin, Borong
Cao, Yuqing
Zhang, Yu
Song, Handa
Huang, Chunhui
Sun, Tianling
Long, Changwen
Liao, Jinling
Zhuo, Kan
CRISPR/Cas9-mediated mutagenesis of the susceptibility gene OsHPP04 in rice confers enhanced resistance to rice root-knot nematode
title CRISPR/Cas9-mediated mutagenesis of the susceptibility gene OsHPP04 in rice confers enhanced resistance to rice root-knot nematode
title_full CRISPR/Cas9-mediated mutagenesis of the susceptibility gene OsHPP04 in rice confers enhanced resistance to rice root-knot nematode
title_fullStr CRISPR/Cas9-mediated mutagenesis of the susceptibility gene OsHPP04 in rice confers enhanced resistance to rice root-knot nematode
title_full_unstemmed CRISPR/Cas9-mediated mutagenesis of the susceptibility gene OsHPP04 in rice confers enhanced resistance to rice root-knot nematode
title_short CRISPR/Cas9-mediated mutagenesis of the susceptibility gene OsHPP04 in rice confers enhanced resistance to rice root-knot nematode
title_sort crispr/cas9-mediated mutagenesis of the susceptibility gene oshpp04 in rice confers enhanced resistance to rice root-knot nematode
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043372/
https://www.ncbi.nlm.nih.gov/pubmed/36998699
http://dx.doi.org/10.3389/fpls.2023.1134653
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