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Antibacterial properties of antimicrobial peptide HHC36 modified polyetheretherketone
INTRODUCTION: Polyetheretherketone (PEEK) is considered to be a new type of orthopedic implant material due to its mechanical properties and biocompatibility. It is becoming a replacement for titanium (Ti) due to its near-human-cortical transmission and modulus of elasticity. However, its clinical a...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043374/ https://www.ncbi.nlm.nih.gov/pubmed/36998411 http://dx.doi.org/10.3389/fmicb.2023.1103956 |
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author | Gao, Weijia Han, Xiao Sun, Duo Li, Yongli Liu, Xiaoli Yang, Shihui Zhou, Zhe Qi, Yuanzheng Jiao, Junjie Zhao, Jinghui |
author_facet | Gao, Weijia Han, Xiao Sun, Duo Li, Yongli Liu, Xiaoli Yang, Shihui Zhou, Zhe Qi, Yuanzheng Jiao, Junjie Zhao, Jinghui |
author_sort | Gao, Weijia |
collection | PubMed |
description | INTRODUCTION: Polyetheretherketone (PEEK) is considered to be a new type of orthopedic implant material due to its mechanical properties and biocompatibility. It is becoming a replacement for titanium (Ti) due to its near-human-cortical transmission and modulus of elasticity. However, its clinical application is limited because of its biological inertia and susceptibility to bacterial infection during implantation. To solve this problem, there is an urgent need to improve the antibacterial properties of PEEK implants. METHODS: In this work, we fixed antimicrobial peptide HHC36 on the 3D porous structure of sulfonated PEEK (SPEEK) by a simple solvent evaporation method (HSPEEK), and carried out characterization tests. We evaluated the antibacterial properties and cytocompatibility of the samples in vitro. In addition, we evaluated the anti-infection property and biocompatibility of the samples in vivo by establishing a rat subcutaneous infection model. RESULTS: The characterization test results showed that HHC36 was successfully fixed on the surface of SPEEK and released slowly for 10 days. The results of antibacterial experiments in vitro showed that HSPEEK could reduce the survival rate of free bacteria, inhibit the growth of bacteria around the sample, and inhibit the formation of biofilm on the sample surface. The cytocompatibility test in vitro showed that the sample had no significant effect on the proliferation and viability of L929 cells and had no hemolytic activity on rabbit erythrocytes. In vivo experiments, HSPEEK can significantly reduce the bacterial survival rate on the sample surface and the inflammatory reaction in the soft tissue around the sample. DISCUSSION: We successfully loaded HHC36 onto the surface of SPEEK through a simple solvent evaporation method. The sample has excellent antibacterial properties and good cell compatibility, which can significantly reduce the bacterial survival rate and inflammatory reaction in vivo. The above results indicated that we successfully improved the antibacterial property of PEEK by a simple modification strategy, making it a promising material for anti-infection orthopedic implants. |
format | Online Article Text |
id | pubmed-10043374 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100433742023-03-29 Antibacterial properties of antimicrobial peptide HHC36 modified polyetheretherketone Gao, Weijia Han, Xiao Sun, Duo Li, Yongli Liu, Xiaoli Yang, Shihui Zhou, Zhe Qi, Yuanzheng Jiao, Junjie Zhao, Jinghui Front Microbiol Microbiology INTRODUCTION: Polyetheretherketone (PEEK) is considered to be a new type of orthopedic implant material due to its mechanical properties and biocompatibility. It is becoming a replacement for titanium (Ti) due to its near-human-cortical transmission and modulus of elasticity. However, its clinical application is limited because of its biological inertia and susceptibility to bacterial infection during implantation. To solve this problem, there is an urgent need to improve the antibacterial properties of PEEK implants. METHODS: In this work, we fixed antimicrobial peptide HHC36 on the 3D porous structure of sulfonated PEEK (SPEEK) by a simple solvent evaporation method (HSPEEK), and carried out characterization tests. We evaluated the antibacterial properties and cytocompatibility of the samples in vitro. In addition, we evaluated the anti-infection property and biocompatibility of the samples in vivo by establishing a rat subcutaneous infection model. RESULTS: The characterization test results showed that HHC36 was successfully fixed on the surface of SPEEK and released slowly for 10 days. The results of antibacterial experiments in vitro showed that HSPEEK could reduce the survival rate of free bacteria, inhibit the growth of bacteria around the sample, and inhibit the formation of biofilm on the sample surface. The cytocompatibility test in vitro showed that the sample had no significant effect on the proliferation and viability of L929 cells and had no hemolytic activity on rabbit erythrocytes. In vivo experiments, HSPEEK can significantly reduce the bacterial survival rate on the sample surface and the inflammatory reaction in the soft tissue around the sample. DISCUSSION: We successfully loaded HHC36 onto the surface of SPEEK through a simple solvent evaporation method. The sample has excellent antibacterial properties and good cell compatibility, which can significantly reduce the bacterial survival rate and inflammatory reaction in vivo. The above results indicated that we successfully improved the antibacterial property of PEEK by a simple modification strategy, making it a promising material for anti-infection orthopedic implants. Frontiers Media S.A. 2023-03-14 /pmc/articles/PMC10043374/ /pubmed/36998411 http://dx.doi.org/10.3389/fmicb.2023.1103956 Text en Copyright © 2023 Gao, Han, Sun, Li, Liu, Yang, Zhou, Qi, Jiao and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Gao, Weijia Han, Xiao Sun, Duo Li, Yongli Liu, Xiaoli Yang, Shihui Zhou, Zhe Qi, Yuanzheng Jiao, Junjie Zhao, Jinghui Antibacterial properties of antimicrobial peptide HHC36 modified polyetheretherketone |
title | Antibacterial properties of antimicrobial peptide HHC36 modified polyetheretherketone |
title_full | Antibacterial properties of antimicrobial peptide HHC36 modified polyetheretherketone |
title_fullStr | Antibacterial properties of antimicrobial peptide HHC36 modified polyetheretherketone |
title_full_unstemmed | Antibacterial properties of antimicrobial peptide HHC36 modified polyetheretherketone |
title_short | Antibacterial properties of antimicrobial peptide HHC36 modified polyetheretherketone |
title_sort | antibacterial properties of antimicrobial peptide hhc36 modified polyetheretherketone |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043374/ https://www.ncbi.nlm.nih.gov/pubmed/36998411 http://dx.doi.org/10.3389/fmicb.2023.1103956 |
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