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Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes

PURPOSE: To investigate differences in outflow facility between angiographically determined high- and low-flow segments of the conventional outflow pathway in porcine eyes. METHODS: Porcine anterior segments (n = 14) were mounted in a perfusion chamber and perfused using Dulbecco's phosphate bu...

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Autores principales: Strohmaier, Clemens A., McDonnell, Fiona S., Zhang, Xiaowei, Wanderer, Daniel, Stamer, W. Daniel, Weinreb, Robert N., Huang, Alex S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043501/
https://www.ncbi.nlm.nih.gov/pubmed/36939719
http://dx.doi.org/10.1167/iovs.64.3.29
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author Strohmaier, Clemens A.
McDonnell, Fiona S.
Zhang, Xiaowei
Wanderer, Daniel
Stamer, W. Daniel
Weinreb, Robert N.
Huang, Alex S.
author_facet Strohmaier, Clemens A.
McDonnell, Fiona S.
Zhang, Xiaowei
Wanderer, Daniel
Stamer, W. Daniel
Weinreb, Robert N.
Huang, Alex S.
author_sort Strohmaier, Clemens A.
collection PubMed
description PURPOSE: To investigate differences in outflow facility between angiographically determined high- and low-flow segments of the conventional outflow pathway in porcine eyes. METHODS: Porcine anterior segments (n = 14) were mounted in a perfusion chamber and perfused using Dulbecco's phosphate buffered solution with glucose. Fluorescein angiography was performed to determine high- and low-flow regions of the conventional outflow pathways. The trabecular meshwork (TM) was occluded using cyanoacrylate glue, except for residual 5-mm TM areas that were either high or low flow at baseline, designating these eyes as “residual high-flow” or “residual low-flow” eyes. Subsequently, outflow was quantitatively reassessed and compared between residual high-flow and residual low-flow eyes followed by indocyanine green angiography. RESULTS: Fluorescein aqueous angiography demonstrated high-flow and low-flow regions. Baseline outflow facilities were 0.320 ± 0.08 and 0.328 ± 0.10 µL/min/mmHg (P = 0.676) in residual high-flow and residual low-flow eyes before TM occlusion, respectively. After partial trabecular meshwork occlusion, outflow facility decreased to 0.209 ± 0.07 µL/min/mmHg (−32.66% ± 19.53%) and 0.114 ± 0.08 µL/min/mmHg (−66.57% ± 23.08%) in residual high- and low-flow eyes (P = 0.035), respectively. There was a significant difference in the resulting IOP increase (P = 0.034). CONCLUSIONS: Angiographically determined high- and low-flow regions in the conventional outflow pathways differ in their segmental outflow facility; thus, there is an uneven distribution of local outflow facility across different parts of the TM.
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spelling pubmed-100435012023-03-29 Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes Strohmaier, Clemens A. McDonnell, Fiona S. Zhang, Xiaowei Wanderer, Daniel Stamer, W. Daniel Weinreb, Robert N. Huang, Alex S. Invest Ophthalmol Vis Sci Physiology and Pharmacology PURPOSE: To investigate differences in outflow facility between angiographically determined high- and low-flow segments of the conventional outflow pathway in porcine eyes. METHODS: Porcine anterior segments (n = 14) were mounted in a perfusion chamber and perfused using Dulbecco's phosphate buffered solution with glucose. Fluorescein angiography was performed to determine high- and low-flow regions of the conventional outflow pathways. The trabecular meshwork (TM) was occluded using cyanoacrylate glue, except for residual 5-mm TM areas that were either high or low flow at baseline, designating these eyes as “residual high-flow” or “residual low-flow” eyes. Subsequently, outflow was quantitatively reassessed and compared between residual high-flow and residual low-flow eyes followed by indocyanine green angiography. RESULTS: Fluorescein aqueous angiography demonstrated high-flow and low-flow regions. Baseline outflow facilities were 0.320 ± 0.08 and 0.328 ± 0.10 µL/min/mmHg (P = 0.676) in residual high-flow and residual low-flow eyes before TM occlusion, respectively. After partial trabecular meshwork occlusion, outflow facility decreased to 0.209 ± 0.07 µL/min/mmHg (−32.66% ± 19.53%) and 0.114 ± 0.08 µL/min/mmHg (−66.57% ± 23.08%) in residual high- and low-flow eyes (P = 0.035), respectively. There was a significant difference in the resulting IOP increase (P = 0.034). CONCLUSIONS: Angiographically determined high- and low-flow regions in the conventional outflow pathways differ in their segmental outflow facility; thus, there is an uneven distribution of local outflow facility across different parts of the TM. The Association for Research in Vision and Ophthalmology 2023-03-20 /pmc/articles/PMC10043501/ /pubmed/36939719 http://dx.doi.org/10.1167/iovs.64.3.29 Text en Copyright 2023 The Authors https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License.
spellingShingle Physiology and Pharmacology
Strohmaier, Clemens A.
McDonnell, Fiona S.
Zhang, Xiaowei
Wanderer, Daniel
Stamer, W. Daniel
Weinreb, Robert N.
Huang, Alex S.
Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes
title Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes
title_full Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes
title_fullStr Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes
title_full_unstemmed Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes
title_short Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes
title_sort differences in outflow facility between angiographically identified high- versus low-flow regions of the conventional outflow pathways in porcine eyes
topic Physiology and Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043501/
https://www.ncbi.nlm.nih.gov/pubmed/36939719
http://dx.doi.org/10.1167/iovs.64.3.29
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