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Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes
PURPOSE: To investigate differences in outflow facility between angiographically determined high- and low-flow segments of the conventional outflow pathway in porcine eyes. METHODS: Porcine anterior segments (n = 14) were mounted in a perfusion chamber and perfused using Dulbecco's phosphate bu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Association for Research in Vision and Ophthalmology
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043501/ https://www.ncbi.nlm.nih.gov/pubmed/36939719 http://dx.doi.org/10.1167/iovs.64.3.29 |
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author | Strohmaier, Clemens A. McDonnell, Fiona S. Zhang, Xiaowei Wanderer, Daniel Stamer, W. Daniel Weinreb, Robert N. Huang, Alex S. |
author_facet | Strohmaier, Clemens A. McDonnell, Fiona S. Zhang, Xiaowei Wanderer, Daniel Stamer, W. Daniel Weinreb, Robert N. Huang, Alex S. |
author_sort | Strohmaier, Clemens A. |
collection | PubMed |
description | PURPOSE: To investigate differences in outflow facility between angiographically determined high- and low-flow segments of the conventional outflow pathway in porcine eyes. METHODS: Porcine anterior segments (n = 14) were mounted in a perfusion chamber and perfused using Dulbecco's phosphate buffered solution with glucose. Fluorescein angiography was performed to determine high- and low-flow regions of the conventional outflow pathways. The trabecular meshwork (TM) was occluded using cyanoacrylate glue, except for residual 5-mm TM areas that were either high or low flow at baseline, designating these eyes as “residual high-flow” or “residual low-flow” eyes. Subsequently, outflow was quantitatively reassessed and compared between residual high-flow and residual low-flow eyes followed by indocyanine green angiography. RESULTS: Fluorescein aqueous angiography demonstrated high-flow and low-flow regions. Baseline outflow facilities were 0.320 ± 0.08 and 0.328 ± 0.10 µL/min/mmHg (P = 0.676) in residual high-flow and residual low-flow eyes before TM occlusion, respectively. After partial trabecular meshwork occlusion, outflow facility decreased to 0.209 ± 0.07 µL/min/mmHg (−32.66% ± 19.53%) and 0.114 ± 0.08 µL/min/mmHg (−66.57% ± 23.08%) in residual high- and low-flow eyes (P = 0.035), respectively. There was a significant difference in the resulting IOP increase (P = 0.034). CONCLUSIONS: Angiographically determined high- and low-flow regions in the conventional outflow pathways differ in their segmental outflow facility; thus, there is an uneven distribution of local outflow facility across different parts of the TM. |
format | Online Article Text |
id | pubmed-10043501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-100435012023-03-29 Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes Strohmaier, Clemens A. McDonnell, Fiona S. Zhang, Xiaowei Wanderer, Daniel Stamer, W. Daniel Weinreb, Robert N. Huang, Alex S. Invest Ophthalmol Vis Sci Physiology and Pharmacology PURPOSE: To investigate differences in outflow facility between angiographically determined high- and low-flow segments of the conventional outflow pathway in porcine eyes. METHODS: Porcine anterior segments (n = 14) were mounted in a perfusion chamber and perfused using Dulbecco's phosphate buffered solution with glucose. Fluorescein angiography was performed to determine high- and low-flow regions of the conventional outflow pathways. The trabecular meshwork (TM) was occluded using cyanoacrylate glue, except for residual 5-mm TM areas that were either high or low flow at baseline, designating these eyes as “residual high-flow” or “residual low-flow” eyes. Subsequently, outflow was quantitatively reassessed and compared between residual high-flow and residual low-flow eyes followed by indocyanine green angiography. RESULTS: Fluorescein aqueous angiography demonstrated high-flow and low-flow regions. Baseline outflow facilities were 0.320 ± 0.08 and 0.328 ± 0.10 µL/min/mmHg (P = 0.676) in residual high-flow and residual low-flow eyes before TM occlusion, respectively. After partial trabecular meshwork occlusion, outflow facility decreased to 0.209 ± 0.07 µL/min/mmHg (−32.66% ± 19.53%) and 0.114 ± 0.08 µL/min/mmHg (−66.57% ± 23.08%) in residual high- and low-flow eyes (P = 0.035), respectively. There was a significant difference in the resulting IOP increase (P = 0.034). CONCLUSIONS: Angiographically determined high- and low-flow regions in the conventional outflow pathways differ in their segmental outflow facility; thus, there is an uneven distribution of local outflow facility across different parts of the TM. The Association for Research in Vision and Ophthalmology 2023-03-20 /pmc/articles/PMC10043501/ /pubmed/36939719 http://dx.doi.org/10.1167/iovs.64.3.29 Text en Copyright 2023 The Authors https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License. |
spellingShingle | Physiology and Pharmacology Strohmaier, Clemens A. McDonnell, Fiona S. Zhang, Xiaowei Wanderer, Daniel Stamer, W. Daniel Weinreb, Robert N. Huang, Alex S. Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes |
title | Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes |
title_full | Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes |
title_fullStr | Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes |
title_full_unstemmed | Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes |
title_short | Differences in Outflow Facility Between Angiographically Identified High- Versus Low-Flow Regions of the Conventional Outflow Pathways in Porcine Eyes |
title_sort | differences in outflow facility between angiographically identified high- versus low-flow regions of the conventional outflow pathways in porcine eyes |
topic | Physiology and Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043501/ https://www.ncbi.nlm.nih.gov/pubmed/36939719 http://dx.doi.org/10.1167/iovs.64.3.29 |
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