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Electrical Broth Micro-Dilution for Rapid Antibiotic Resistance Testing
[Image: see text] Rapid tests to assess the susceptibility of bacteria to antibiotics are required to inform antibiotic stewardship. We have developed a novel test, which measures changes in the impedance of a 100 nanoliter volume of bacterial suspension to determine an “electrical” minimum inhibito...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043929/ https://www.ncbi.nlm.nih.gov/pubmed/36820613 http://dx.doi.org/10.1021/acssensors.2c02166 |
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author | Spencer, Daniel Li, Yuetao Zhu, Yiling Sutton, J. Mark Morgan, Hywel |
author_facet | Spencer, Daniel Li, Yuetao Zhu, Yiling Sutton, J. Mark Morgan, Hywel |
author_sort | Spencer, Daniel |
collection | PubMed |
description | [Image: see text] Rapid tests to assess the susceptibility of bacteria to antibiotics are required to inform antibiotic stewardship. We have developed a novel test, which measures changes in the impedance of a 100 nanoliter volume of bacterial suspension to determine an “electrical” minimum inhibitory concentration (eMIC). Two representative strains of Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus were tested against a panel of frontline antibiotics with different modes of action (ciprofloxacin, doxycycline, colistin and imipenem, gentamicin, and ceftazidime). The eMIC measured at 1 h correlated strongly with a standard 24 h microbroth dilution MIC for all combinations of antibiotics and bacteria, allowing strains to be correctly assigned as sensitive or resistant measured in a fraction of the time. |
format | Online Article Text |
id | pubmed-10043929 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-100439292023-03-29 Electrical Broth Micro-Dilution for Rapid Antibiotic Resistance Testing Spencer, Daniel Li, Yuetao Zhu, Yiling Sutton, J. Mark Morgan, Hywel ACS Sens [Image: see text] Rapid tests to assess the susceptibility of bacteria to antibiotics are required to inform antibiotic stewardship. We have developed a novel test, which measures changes in the impedance of a 100 nanoliter volume of bacterial suspension to determine an “electrical” minimum inhibitory concentration (eMIC). Two representative strains of Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus were tested against a panel of frontline antibiotics with different modes of action (ciprofloxacin, doxycycline, colistin and imipenem, gentamicin, and ceftazidime). The eMIC measured at 1 h correlated strongly with a standard 24 h microbroth dilution MIC for all combinations of antibiotics and bacteria, allowing strains to be correctly assigned as sensitive or resistant measured in a fraction of the time. American Chemical Society 2023-02-23 /pmc/articles/PMC10043929/ /pubmed/36820613 http://dx.doi.org/10.1021/acssensors.2c02166 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Spencer, Daniel Li, Yuetao Zhu, Yiling Sutton, J. Mark Morgan, Hywel Electrical Broth Micro-Dilution for Rapid Antibiotic Resistance Testing |
title | Electrical Broth
Micro-Dilution for Rapid Antibiotic
Resistance Testing |
title_full | Electrical Broth
Micro-Dilution for Rapid Antibiotic
Resistance Testing |
title_fullStr | Electrical Broth
Micro-Dilution for Rapid Antibiotic
Resistance Testing |
title_full_unstemmed | Electrical Broth
Micro-Dilution for Rapid Antibiotic
Resistance Testing |
title_short | Electrical Broth
Micro-Dilution for Rapid Antibiotic
Resistance Testing |
title_sort | electrical broth
micro-dilution for rapid antibiotic
resistance testing |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043929/ https://www.ncbi.nlm.nih.gov/pubmed/36820613 http://dx.doi.org/10.1021/acssensors.2c02166 |
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