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Mechanisms of Antimicrobial Peptides from Bagasse against Human Pathogenic Bacteria

Nonedible agricultural wastes (agricultural wastes, agro-industrial wastes, and fishery wastes) were chosen as potential sources of antimicrobial peptides and evaluated for antibacterial efficiency against human pathogens. Specifically, protein hydrolysates were first obtained by hydrolysis with pep...

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Autores principales: Ditsawanon, Thitiporn, Phaonakrob, Narumon, Roytrakul, Sittiruk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044114/
https://www.ncbi.nlm.nih.gov/pubmed/36978315
http://dx.doi.org/10.3390/antibiotics12030448
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author Ditsawanon, Thitiporn
Phaonakrob, Narumon
Roytrakul, Sittiruk
author_facet Ditsawanon, Thitiporn
Phaonakrob, Narumon
Roytrakul, Sittiruk
author_sort Ditsawanon, Thitiporn
collection PubMed
description Nonedible agricultural wastes (agricultural wastes, agro-industrial wastes, and fishery wastes) were chosen as potential sources of antimicrobial peptides and evaluated for antibacterial efficiency against human pathogens. Specifically, protein hydrolysates were first obtained by hydrolysis with pepsin. Filtrated peptides smaller than 3 kDa were then purified by C18 reversed-phase chromatography, cation exchange chromatography, and off-gel fractionation. NanoLC-MS/MS was used to investigate the amino acid sequences of active peptide candidates. Five candidate peptides were finally chosen for chemical synthesis and evaluation of growth inhibition against human pathogenic bacteria. Two synthetic peptides from bagasse, NLWSNEINQDMAEF (Asn-Leu-Trp-Ser-Asn-Glu-Ile-Asn-Gln-Asp-Met-Ala-Glu-Phe) and VSNCL (Val-Ser-Asn-Cys-Leu), showed the most potent antibacterial activity against three pathogens: Pseudomonas aeruginosa, Bacillus subtilis, and Burkholderia cepacia. The antibacterial mechanisms of these peptides were then examined using shotgun proteomics, which revealed their effects to involve both intracellular-active and membrane-active mechanisms. Further investigation and modification of peptides are needed to increase the efficiency of these peptides against human pathogens.
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spelling pubmed-100441142023-03-29 Mechanisms of Antimicrobial Peptides from Bagasse against Human Pathogenic Bacteria Ditsawanon, Thitiporn Phaonakrob, Narumon Roytrakul, Sittiruk Antibiotics (Basel) Article Nonedible agricultural wastes (agricultural wastes, agro-industrial wastes, and fishery wastes) were chosen as potential sources of antimicrobial peptides and evaluated for antibacterial efficiency against human pathogens. Specifically, protein hydrolysates were first obtained by hydrolysis with pepsin. Filtrated peptides smaller than 3 kDa were then purified by C18 reversed-phase chromatography, cation exchange chromatography, and off-gel fractionation. NanoLC-MS/MS was used to investigate the amino acid sequences of active peptide candidates. Five candidate peptides were finally chosen for chemical synthesis and evaluation of growth inhibition against human pathogenic bacteria. Two synthetic peptides from bagasse, NLWSNEINQDMAEF (Asn-Leu-Trp-Ser-Asn-Glu-Ile-Asn-Gln-Asp-Met-Ala-Glu-Phe) and VSNCL (Val-Ser-Asn-Cys-Leu), showed the most potent antibacterial activity against three pathogens: Pseudomonas aeruginosa, Bacillus subtilis, and Burkholderia cepacia. The antibacterial mechanisms of these peptides were then examined using shotgun proteomics, which revealed their effects to involve both intracellular-active and membrane-active mechanisms. Further investigation and modification of peptides are needed to increase the efficiency of these peptides against human pathogens. MDPI 2023-02-23 /pmc/articles/PMC10044114/ /pubmed/36978315 http://dx.doi.org/10.3390/antibiotics12030448 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ditsawanon, Thitiporn
Phaonakrob, Narumon
Roytrakul, Sittiruk
Mechanisms of Antimicrobial Peptides from Bagasse against Human Pathogenic Bacteria
title Mechanisms of Antimicrobial Peptides from Bagasse against Human Pathogenic Bacteria
title_full Mechanisms of Antimicrobial Peptides from Bagasse against Human Pathogenic Bacteria
title_fullStr Mechanisms of Antimicrobial Peptides from Bagasse against Human Pathogenic Bacteria
title_full_unstemmed Mechanisms of Antimicrobial Peptides from Bagasse against Human Pathogenic Bacteria
title_short Mechanisms of Antimicrobial Peptides from Bagasse against Human Pathogenic Bacteria
title_sort mechanisms of antimicrobial peptides from bagasse against human pathogenic bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044114/
https://www.ncbi.nlm.nih.gov/pubmed/36978315
http://dx.doi.org/10.3390/antibiotics12030448
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