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Identification of mcr-1 Genes and Characterization of Resistance Mechanisms to Colistin in Escherichia coli Isolates from Colombian Hospitals

We report the presence of the mcr-1 gene among 880 Escherichia coli clinical isolates collected in 13 hospitals from 12 Colombian cities between 2016 and 2019. Seven (0.8%) isolates were colistin resistant (MIC ≥ 4 µg/mL). These colistin-resistant isolates were screened for the presence of the mcr-1...

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Autores principales: De La Cadena, Elsa, Mahecha, Mateo, Velandia, Ana María, García-Betancur, Juan Carlos, Rojas, Laura J., Porras, Jessica, Pallares, Christian, Villegas, María Virginia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044228/
https://www.ncbi.nlm.nih.gov/pubmed/36978355
http://dx.doi.org/10.3390/antibiotics12030488
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author De La Cadena, Elsa
Mahecha, Mateo
Velandia, Ana María
García-Betancur, Juan Carlos
Rojas, Laura J.
Porras, Jessica
Pallares, Christian
Villegas, María Virginia
author_facet De La Cadena, Elsa
Mahecha, Mateo
Velandia, Ana María
García-Betancur, Juan Carlos
Rojas, Laura J.
Porras, Jessica
Pallares, Christian
Villegas, María Virginia
author_sort De La Cadena, Elsa
collection PubMed
description We report the presence of the mcr-1 gene among 880 Escherichia coli clinical isolates collected in 13 hospitals from 12 Colombian cities between 2016 and 2019. Seven (0.8%) isolates were colistin resistant (MIC ≥ 4 µg/mL). These colistin-resistant isolates were screened for the presence of the mcr-1 gene; five carried the gene. These five isolates were subjected to whole genome sequencing (WGS) to identify additional resistomes and their ST. In addition, antimicrobial susceptibility testing revealed that all E. coli isolates carrying mcr-1 were susceptible to third generation-cephalosporin and carbapenems, except one, which carried an extended-spectrum β-lactamase (CTX-M-55), along with the fosfomycin resistance encoding gene, fosA. WGS indicated that these isolates belonged to four distinct sequence types (ST58, ST46, ST393, and a newly described ST14315) and to phylogroups B1, A, and D. In this geographic region, the spread of mcr-1 in E. coli is low and has not been inserted into high-risk clones such as ST131, which has been present in the country longer.
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spelling pubmed-100442282023-03-29 Identification of mcr-1 Genes and Characterization of Resistance Mechanisms to Colistin in Escherichia coli Isolates from Colombian Hospitals De La Cadena, Elsa Mahecha, Mateo Velandia, Ana María García-Betancur, Juan Carlos Rojas, Laura J. Porras, Jessica Pallares, Christian Villegas, María Virginia Antibiotics (Basel) Article We report the presence of the mcr-1 gene among 880 Escherichia coli clinical isolates collected in 13 hospitals from 12 Colombian cities between 2016 and 2019. Seven (0.8%) isolates were colistin resistant (MIC ≥ 4 µg/mL). These colistin-resistant isolates were screened for the presence of the mcr-1 gene; five carried the gene. These five isolates were subjected to whole genome sequencing (WGS) to identify additional resistomes and their ST. In addition, antimicrobial susceptibility testing revealed that all E. coli isolates carrying mcr-1 were susceptible to third generation-cephalosporin and carbapenems, except one, which carried an extended-spectrum β-lactamase (CTX-M-55), along with the fosfomycin resistance encoding gene, fosA. WGS indicated that these isolates belonged to four distinct sequence types (ST58, ST46, ST393, and a newly described ST14315) and to phylogroups B1, A, and D. In this geographic region, the spread of mcr-1 in E. coli is low and has not been inserted into high-risk clones such as ST131, which has been present in the country longer. MDPI 2023-03-01 /pmc/articles/PMC10044228/ /pubmed/36978355 http://dx.doi.org/10.3390/antibiotics12030488 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
De La Cadena, Elsa
Mahecha, Mateo
Velandia, Ana María
García-Betancur, Juan Carlos
Rojas, Laura J.
Porras, Jessica
Pallares, Christian
Villegas, María Virginia
Identification of mcr-1 Genes and Characterization of Resistance Mechanisms to Colistin in Escherichia coli Isolates from Colombian Hospitals
title Identification of mcr-1 Genes and Characterization of Resistance Mechanisms to Colistin in Escherichia coli Isolates from Colombian Hospitals
title_full Identification of mcr-1 Genes and Characterization of Resistance Mechanisms to Colistin in Escherichia coli Isolates from Colombian Hospitals
title_fullStr Identification of mcr-1 Genes and Characterization of Resistance Mechanisms to Colistin in Escherichia coli Isolates from Colombian Hospitals
title_full_unstemmed Identification of mcr-1 Genes and Characterization of Resistance Mechanisms to Colistin in Escherichia coli Isolates from Colombian Hospitals
title_short Identification of mcr-1 Genes and Characterization of Resistance Mechanisms to Colistin in Escherichia coli Isolates from Colombian Hospitals
title_sort identification of mcr-1 genes and characterization of resistance mechanisms to colistin in escherichia coli isolates from colombian hospitals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044228/
https://www.ncbi.nlm.nih.gov/pubmed/36978355
http://dx.doi.org/10.3390/antibiotics12030488
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