Cargando…

Antimicrobial, Antibiofilm, and Antioxidant Potentials of Four Halophytic Plants, Euphorbia chamaesyce, Bassia arabica, Fagonia mollis, and Haloxylon salicornicum, Growing in Qassim Region of Saudi Arabia: Phytochemical Profile and In Vitro and In Silico Bioactivity Investigations

The current study aimed to investigate the phytochemical contents and antioxidant, antimicrobial, and antibiofilm activities of four halophytic plants, namely, Euphorbia chamaesyce, Bassia arabica, Fagonia mollis, and Haloxylon salicornicum, native to central Saudi Arabia. The alcoholic extract of E...

Descripción completa

Detalles Bibliográficos
Autores principales: Rugaie, Osamah Al, Mohammed, Hamdoon A., Alsamani, Salman, Messaoudi, Sabri, Aroua, Lotfi M., Khan, Riaz A., Almahmoud, Suliman A., Altaleb, Abdulrahman D., Alsharidah, Mansour, Aldubaib, Musaad, Al-Regaiey, Khalid A., Qureshi, Kamal A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044527/
https://www.ncbi.nlm.nih.gov/pubmed/36978368
http://dx.doi.org/10.3390/antibiotics12030501
_version_ 1784913368692293632
author Rugaie, Osamah Al
Mohammed, Hamdoon A.
Alsamani, Salman
Messaoudi, Sabri
Aroua, Lotfi M.
Khan, Riaz A.
Almahmoud, Suliman A.
Altaleb, Abdulrahman D.
Alsharidah, Mansour
Aldubaib, Musaad
Al-Regaiey, Khalid A.
Qureshi, Kamal A.
author_facet Rugaie, Osamah Al
Mohammed, Hamdoon A.
Alsamani, Salman
Messaoudi, Sabri
Aroua, Lotfi M.
Khan, Riaz A.
Almahmoud, Suliman A.
Altaleb, Abdulrahman D.
Alsharidah, Mansour
Aldubaib, Musaad
Al-Regaiey, Khalid A.
Qureshi, Kamal A.
author_sort Rugaie, Osamah Al
collection PubMed
description The current study aimed to investigate the phytochemical contents and antioxidant, antimicrobial, and antibiofilm activities of four halophytic plants, namely, Euphorbia chamaesyce, Bassia arabica, Fagonia mollis, and Haloxylon salicornicum, native to central Saudi Arabia. The alcoholic extract of E. chamaesyce was found to be the most potent in various bioactivities-based evaluations and rich in polyphenols and flavonoid secondary metabolites, with 68.0 mg/g and 39.23 mg/g gallic acid and quercetin equivalents, respectively. Among all plants’ extracts, the alcoholic extract of E. chamaesyce had the highest DPPH scavenging and metal chelating antioxidant activities at 74.15 Trolox equivalents and 16.28 EDTA equivalents, respectively. The highest antimicrobial activity of E. chamaesyce extract was found to be against Shigella flexneri, with a mean zone of inhibition diameter of 18.1 ± 0.2 mm, whereas the minimum inhibitory concentration, minimum biocidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration values were 12.5, 25, 25, and 50 mg/mL, respectively. The LC-ESI-MS/MS analysis of the E. chamaesyce extract showed the presence of six flavonoids and ten phenolic constituents. The in silico binding of the E. chamaesyce extract’s constituents to Staphylococcus aureus tyrosyl-tRNA synthetase enzyme displayed −6.2 to −10.1 kcal/mol binding energy values, suggesting that these constituents can contribute to the antimicrobial properties of the plant extract, making it an essential medicinal ingredient. In conclusion, these results warrant further investigation to standardize the antimicrobial profiles of these plant extracts.
format Online
Article
Text
id pubmed-10044527
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100445272023-03-29 Antimicrobial, Antibiofilm, and Antioxidant Potentials of Four Halophytic Plants, Euphorbia chamaesyce, Bassia arabica, Fagonia mollis, and Haloxylon salicornicum, Growing in Qassim Region of Saudi Arabia: Phytochemical Profile and In Vitro and In Silico Bioactivity Investigations Rugaie, Osamah Al Mohammed, Hamdoon A. Alsamani, Salman Messaoudi, Sabri Aroua, Lotfi M. Khan, Riaz A. Almahmoud, Suliman A. Altaleb, Abdulrahman D. Alsharidah, Mansour Aldubaib, Musaad Al-Regaiey, Khalid A. Qureshi, Kamal A. Antibiotics (Basel) Article The current study aimed to investigate the phytochemical contents and antioxidant, antimicrobial, and antibiofilm activities of four halophytic plants, namely, Euphorbia chamaesyce, Bassia arabica, Fagonia mollis, and Haloxylon salicornicum, native to central Saudi Arabia. The alcoholic extract of E. chamaesyce was found to be the most potent in various bioactivities-based evaluations and rich in polyphenols and flavonoid secondary metabolites, with 68.0 mg/g and 39.23 mg/g gallic acid and quercetin equivalents, respectively. Among all plants’ extracts, the alcoholic extract of E. chamaesyce had the highest DPPH scavenging and metal chelating antioxidant activities at 74.15 Trolox equivalents and 16.28 EDTA equivalents, respectively. The highest antimicrobial activity of E. chamaesyce extract was found to be against Shigella flexneri, with a mean zone of inhibition diameter of 18.1 ± 0.2 mm, whereas the minimum inhibitory concentration, minimum biocidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration values were 12.5, 25, 25, and 50 mg/mL, respectively. The LC-ESI-MS/MS analysis of the E. chamaesyce extract showed the presence of six flavonoids and ten phenolic constituents. The in silico binding of the E. chamaesyce extract’s constituents to Staphylococcus aureus tyrosyl-tRNA synthetase enzyme displayed −6.2 to −10.1 kcal/mol binding energy values, suggesting that these constituents can contribute to the antimicrobial properties of the plant extract, making it an essential medicinal ingredient. In conclusion, these results warrant further investigation to standardize the antimicrobial profiles of these plant extracts. MDPI 2023-03-02 /pmc/articles/PMC10044527/ /pubmed/36978368 http://dx.doi.org/10.3390/antibiotics12030501 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rugaie, Osamah Al
Mohammed, Hamdoon A.
Alsamani, Salman
Messaoudi, Sabri
Aroua, Lotfi M.
Khan, Riaz A.
Almahmoud, Suliman A.
Altaleb, Abdulrahman D.
Alsharidah, Mansour
Aldubaib, Musaad
Al-Regaiey, Khalid A.
Qureshi, Kamal A.
Antimicrobial, Antibiofilm, and Antioxidant Potentials of Four Halophytic Plants, Euphorbia chamaesyce, Bassia arabica, Fagonia mollis, and Haloxylon salicornicum, Growing in Qassim Region of Saudi Arabia: Phytochemical Profile and In Vitro and In Silico Bioactivity Investigations
title Antimicrobial, Antibiofilm, and Antioxidant Potentials of Four Halophytic Plants, Euphorbia chamaesyce, Bassia arabica, Fagonia mollis, and Haloxylon salicornicum, Growing in Qassim Region of Saudi Arabia: Phytochemical Profile and In Vitro and In Silico Bioactivity Investigations
title_full Antimicrobial, Antibiofilm, and Antioxidant Potentials of Four Halophytic Plants, Euphorbia chamaesyce, Bassia arabica, Fagonia mollis, and Haloxylon salicornicum, Growing in Qassim Region of Saudi Arabia: Phytochemical Profile and In Vitro and In Silico Bioactivity Investigations
title_fullStr Antimicrobial, Antibiofilm, and Antioxidant Potentials of Four Halophytic Plants, Euphorbia chamaesyce, Bassia arabica, Fagonia mollis, and Haloxylon salicornicum, Growing in Qassim Region of Saudi Arabia: Phytochemical Profile and In Vitro and In Silico Bioactivity Investigations
title_full_unstemmed Antimicrobial, Antibiofilm, and Antioxidant Potentials of Four Halophytic Plants, Euphorbia chamaesyce, Bassia arabica, Fagonia mollis, and Haloxylon salicornicum, Growing in Qassim Region of Saudi Arabia: Phytochemical Profile and In Vitro and In Silico Bioactivity Investigations
title_short Antimicrobial, Antibiofilm, and Antioxidant Potentials of Four Halophytic Plants, Euphorbia chamaesyce, Bassia arabica, Fagonia mollis, and Haloxylon salicornicum, Growing in Qassim Region of Saudi Arabia: Phytochemical Profile and In Vitro and In Silico Bioactivity Investigations
title_sort antimicrobial, antibiofilm, and antioxidant potentials of four halophytic plants, euphorbia chamaesyce, bassia arabica, fagonia mollis, and haloxylon salicornicum, growing in qassim region of saudi arabia: phytochemical profile and in vitro and in silico bioactivity investigations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044527/
https://www.ncbi.nlm.nih.gov/pubmed/36978368
http://dx.doi.org/10.3390/antibiotics12030501
work_keys_str_mv AT rugaieosamahal antimicrobialantibiofilmandantioxidantpotentialsoffourhalophyticplantseuphorbiachamaesycebassiaarabicafagoniamollisandhaloxylonsalicornicumgrowinginqassimregionofsaudiarabiaphytochemicalprofileandinvitroandinsilicobioactivityinvestigations
AT mohammedhamdoona antimicrobialantibiofilmandantioxidantpotentialsoffourhalophyticplantseuphorbiachamaesycebassiaarabicafagoniamollisandhaloxylonsalicornicumgrowinginqassimregionofsaudiarabiaphytochemicalprofileandinvitroandinsilicobioactivityinvestigations
AT alsamanisalman antimicrobialantibiofilmandantioxidantpotentialsoffourhalophyticplantseuphorbiachamaesycebassiaarabicafagoniamollisandhaloxylonsalicornicumgrowinginqassimregionofsaudiarabiaphytochemicalprofileandinvitroandinsilicobioactivityinvestigations
AT messaoudisabri antimicrobialantibiofilmandantioxidantpotentialsoffourhalophyticplantseuphorbiachamaesycebassiaarabicafagoniamollisandhaloxylonsalicornicumgrowinginqassimregionofsaudiarabiaphytochemicalprofileandinvitroandinsilicobioactivityinvestigations
AT aroualotfim antimicrobialantibiofilmandantioxidantpotentialsoffourhalophyticplantseuphorbiachamaesycebassiaarabicafagoniamollisandhaloxylonsalicornicumgrowinginqassimregionofsaudiarabiaphytochemicalprofileandinvitroandinsilicobioactivityinvestigations
AT khanriaza antimicrobialantibiofilmandantioxidantpotentialsoffourhalophyticplantseuphorbiachamaesycebassiaarabicafagoniamollisandhaloxylonsalicornicumgrowinginqassimregionofsaudiarabiaphytochemicalprofileandinvitroandinsilicobioactivityinvestigations
AT almahmoudsulimana antimicrobialantibiofilmandantioxidantpotentialsoffourhalophyticplantseuphorbiachamaesycebassiaarabicafagoniamollisandhaloxylonsalicornicumgrowinginqassimregionofsaudiarabiaphytochemicalprofileandinvitroandinsilicobioactivityinvestigations
AT altalebabdulrahmand antimicrobialantibiofilmandantioxidantpotentialsoffourhalophyticplantseuphorbiachamaesycebassiaarabicafagoniamollisandhaloxylonsalicornicumgrowinginqassimregionofsaudiarabiaphytochemicalprofileandinvitroandinsilicobioactivityinvestigations
AT alsharidahmansour antimicrobialantibiofilmandantioxidantpotentialsoffourhalophyticplantseuphorbiachamaesycebassiaarabicafagoniamollisandhaloxylonsalicornicumgrowinginqassimregionofsaudiarabiaphytochemicalprofileandinvitroandinsilicobioactivityinvestigations
AT aldubaibmusaad antimicrobialantibiofilmandantioxidantpotentialsoffourhalophyticplantseuphorbiachamaesycebassiaarabicafagoniamollisandhaloxylonsalicornicumgrowinginqassimregionofsaudiarabiaphytochemicalprofileandinvitroandinsilicobioactivityinvestigations
AT alregaieykhalida antimicrobialantibiofilmandantioxidantpotentialsoffourhalophyticplantseuphorbiachamaesycebassiaarabicafagoniamollisandhaloxylonsalicornicumgrowinginqassimregionofsaudiarabiaphytochemicalprofileandinvitroandinsilicobioactivityinvestigations
AT qureshikamala antimicrobialantibiofilmandantioxidantpotentialsoffourhalophyticplantseuphorbiachamaesycebassiaarabicafagoniamollisandhaloxylonsalicornicumgrowinginqassimregionofsaudiarabiaphytochemicalprofileandinvitroandinsilicobioactivityinvestigations