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Preparation and Evaluation of Nanoemulsion of Citronella Essential Oil with Improved Antimicrobial and Anti-Cancer Properties

The development of new pharmaceutical solutions for treating various diseases results from a growing understanding of the benefits of using essential oils. One of the most often used volatile materials among essential oils is the oil of the citronella plant, termed citronella essential oil (CITEO),...

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Autores principales: Jawaid, Talha, Alaseem, Ali Mohammed, Khan, Mohammed Moizuddin, Mukhtar, Beenish, Kamal, Mehnaz, Anwer, Razique, Ahmed, Saif, Alam, Aftab
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044558/
https://www.ncbi.nlm.nih.gov/pubmed/36978345
http://dx.doi.org/10.3390/antibiotics12030478
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author Jawaid, Talha
Alaseem, Ali Mohammed
Khan, Mohammed Moizuddin
Mukhtar, Beenish
Kamal, Mehnaz
Anwer, Razique
Ahmed, Saif
Alam, Aftab
author_facet Jawaid, Talha
Alaseem, Ali Mohammed
Khan, Mohammed Moizuddin
Mukhtar, Beenish
Kamal, Mehnaz
Anwer, Razique
Ahmed, Saif
Alam, Aftab
author_sort Jawaid, Talha
collection PubMed
description The development of new pharmaceutical solutions for treating various diseases results from a growing understanding of the benefits of using essential oils. One of the most often used volatile materials among essential oils is the oil of the citronella plant, termed citronella essential oil (CITEO), which has potential for use in food and medicine. Its wide use is limited due to lipophilicity, high volatility and poor physicochemical stability. With this background, the present study aims to evaluate the properties of CITEO-nanoemulsion (CITEO-NE) by analyzing its antimicrobial activities against Staphylococcus aureus (S. aureus) and Candida albicans (C. albicans) and its anticancer activity against, human skin adenocarcinoma cell line (A431). The CITEO-NE was prepared and evaluated for the size range of 130 ± 5 nm, polydispersity index (PDI) of 0.127 and zeta potential −12.6 mV. The percentage % of entrapment efficiency (%EE) of nanoemulsions loaded with CIT was very high at the beginning of the study, at 95.5 ± 4.775%. The MIC was observed to be 500 µg/mL for CITEO and 250 µg/mL for CITEO-NE against S. aureus and 250 µg/mL for CITEO and 125 µg/mL for CITEO-NE against C. albicans. The time-kill assay also suggests the effectiveness of CITEO-NE against the test pathogens as a novel alternative therapy. The IC(50) values of CITEO and CITEO-NE exhibited significant cytotoxic properties against the A431 cell line, with 41.20 μg/mL and 37.71 μg/mL, respectively. Hence, our findings revealed that encapsulation of CITEO increased the pharmacological properties.
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spelling pubmed-100445582023-03-29 Preparation and Evaluation of Nanoemulsion of Citronella Essential Oil with Improved Antimicrobial and Anti-Cancer Properties Jawaid, Talha Alaseem, Ali Mohammed Khan, Mohammed Moizuddin Mukhtar, Beenish Kamal, Mehnaz Anwer, Razique Ahmed, Saif Alam, Aftab Antibiotics (Basel) Article The development of new pharmaceutical solutions for treating various diseases results from a growing understanding of the benefits of using essential oils. One of the most often used volatile materials among essential oils is the oil of the citronella plant, termed citronella essential oil (CITEO), which has potential for use in food and medicine. Its wide use is limited due to lipophilicity, high volatility and poor physicochemical stability. With this background, the present study aims to evaluate the properties of CITEO-nanoemulsion (CITEO-NE) by analyzing its antimicrobial activities against Staphylococcus aureus (S. aureus) and Candida albicans (C. albicans) and its anticancer activity against, human skin adenocarcinoma cell line (A431). The CITEO-NE was prepared and evaluated for the size range of 130 ± 5 nm, polydispersity index (PDI) of 0.127 and zeta potential −12.6 mV. The percentage % of entrapment efficiency (%EE) of nanoemulsions loaded with CIT was very high at the beginning of the study, at 95.5 ± 4.775%. The MIC was observed to be 500 µg/mL for CITEO and 250 µg/mL for CITEO-NE against S. aureus and 250 µg/mL for CITEO and 125 µg/mL for CITEO-NE against C. albicans. The time-kill assay also suggests the effectiveness of CITEO-NE against the test pathogens as a novel alternative therapy. The IC(50) values of CITEO and CITEO-NE exhibited significant cytotoxic properties against the A431 cell line, with 41.20 μg/mL and 37.71 μg/mL, respectively. Hence, our findings revealed that encapsulation of CITEO increased the pharmacological properties. MDPI 2023-02-27 /pmc/articles/PMC10044558/ /pubmed/36978345 http://dx.doi.org/10.3390/antibiotics12030478 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jawaid, Talha
Alaseem, Ali Mohammed
Khan, Mohammed Moizuddin
Mukhtar, Beenish
Kamal, Mehnaz
Anwer, Razique
Ahmed, Saif
Alam, Aftab
Preparation and Evaluation of Nanoemulsion of Citronella Essential Oil with Improved Antimicrobial and Anti-Cancer Properties
title Preparation and Evaluation of Nanoemulsion of Citronella Essential Oil with Improved Antimicrobial and Anti-Cancer Properties
title_full Preparation and Evaluation of Nanoemulsion of Citronella Essential Oil with Improved Antimicrobial and Anti-Cancer Properties
title_fullStr Preparation and Evaluation of Nanoemulsion of Citronella Essential Oil with Improved Antimicrobial and Anti-Cancer Properties
title_full_unstemmed Preparation and Evaluation of Nanoemulsion of Citronella Essential Oil with Improved Antimicrobial and Anti-Cancer Properties
title_short Preparation and Evaluation of Nanoemulsion of Citronella Essential Oil with Improved Antimicrobial and Anti-Cancer Properties
title_sort preparation and evaluation of nanoemulsion of citronella essential oil with improved antimicrobial and anti-cancer properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044558/
https://www.ncbi.nlm.nih.gov/pubmed/36978345
http://dx.doi.org/10.3390/antibiotics12030478
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