Cargando…

Photoactivated antibiotics to treat intracellular infection of bacteria

Antibiotic resistance combined with pathogen internalization leads to debilitating infections. Here we test novel superoxide producing, stimuli-activated quantum dots (QDs), to treat an intracellular infection of Salmonella enterica serovar Typhimurium in an osteoblast precursor cell line. These QDs...

Descripción completa

Detalles Bibliográficos
Autores principales: Eller, Kristen A., Stamo, Dana F., McCollum, Colleen R., Campos, Jocelyn K., Levy, Max, Nagpal, Prashant, Chatterjee, Anushree
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044578/
https://www.ncbi.nlm.nih.gov/pubmed/36998655
http://dx.doi.org/10.1039/d2na00378c
_version_ 1784913381051858944
author Eller, Kristen A.
Stamo, Dana F.
McCollum, Colleen R.
Campos, Jocelyn K.
Levy, Max
Nagpal, Prashant
Chatterjee, Anushree
author_facet Eller, Kristen A.
Stamo, Dana F.
McCollum, Colleen R.
Campos, Jocelyn K.
Levy, Max
Nagpal, Prashant
Chatterjee, Anushree
author_sort Eller, Kristen A.
collection PubMed
description Antibiotic resistance combined with pathogen internalization leads to debilitating infections. Here we test novel superoxide producing, stimuli-activated quantum dots (QDs), to treat an intracellular infection of Salmonella enterica serovar Typhimurium in an osteoblast precursor cell line. These QDs are precisely tuned to reduce dissolved oxygen to superoxide and kill bacteria upon stimulation (e.g., light). We show QDs provide tunable clearance at various multiplicities of infection and limited host cell toxicity by modulating their concentration and stimuli intensity, proving the efficacy of superoxide producing QDs for intracellular infection treatment and establishing a framework for further testing in different infection models.
format Online
Article
Text
id pubmed-10044578
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher RSC
record_format MEDLINE/PubMed
spelling pubmed-100445782023-03-29 Photoactivated antibiotics to treat intracellular infection of bacteria Eller, Kristen A. Stamo, Dana F. McCollum, Colleen R. Campos, Jocelyn K. Levy, Max Nagpal, Prashant Chatterjee, Anushree Nanoscale Adv Chemistry Antibiotic resistance combined with pathogen internalization leads to debilitating infections. Here we test novel superoxide producing, stimuli-activated quantum dots (QDs), to treat an intracellular infection of Salmonella enterica serovar Typhimurium in an osteoblast precursor cell line. These QDs are precisely tuned to reduce dissolved oxygen to superoxide and kill bacteria upon stimulation (e.g., light). We show QDs provide tunable clearance at various multiplicities of infection and limited host cell toxicity by modulating their concentration and stimuli intensity, proving the efficacy of superoxide producing QDs for intracellular infection treatment and establishing a framework for further testing in different infection models. RSC 2023-03-07 /pmc/articles/PMC10044578/ /pubmed/36998655 http://dx.doi.org/10.1039/d2na00378c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Eller, Kristen A.
Stamo, Dana F.
McCollum, Colleen R.
Campos, Jocelyn K.
Levy, Max
Nagpal, Prashant
Chatterjee, Anushree
Photoactivated antibiotics to treat intracellular infection of bacteria
title Photoactivated antibiotics to treat intracellular infection of bacteria
title_full Photoactivated antibiotics to treat intracellular infection of bacteria
title_fullStr Photoactivated antibiotics to treat intracellular infection of bacteria
title_full_unstemmed Photoactivated antibiotics to treat intracellular infection of bacteria
title_short Photoactivated antibiotics to treat intracellular infection of bacteria
title_sort photoactivated antibiotics to treat intracellular infection of bacteria
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044578/
https://www.ncbi.nlm.nih.gov/pubmed/36998655
http://dx.doi.org/10.1039/d2na00378c
work_keys_str_mv AT ellerkristena photoactivatedantibioticstotreatintracellularinfectionofbacteria
AT stamodanaf photoactivatedantibioticstotreatintracellularinfectionofbacteria
AT mccollumcolleenr photoactivatedantibioticstotreatintracellularinfectionofbacteria
AT camposjocelynk photoactivatedantibioticstotreatintracellularinfectionofbacteria
AT levymax photoactivatedantibioticstotreatintracellularinfectionofbacteria
AT nagpalprashant photoactivatedantibioticstotreatintracellularinfectionofbacteria
AT chatterjeeanushree photoactivatedantibioticstotreatintracellularinfectionofbacteria