Effects of Compound Probiotics on Cecal Microbiota and Metabolome of Swine

SIMPLE SUMMARY: In this study, we used a new mixture to generate probiotics and significantly reduced the relative abundance of Paraprevotella and Phascolarctobacterium, and the group fed with this mixture had higher relative abundance of Prevotella, Faecaliberium, and Gemmiger. These had a big impact on the metabolic pathways. The aim of our study was to use 16S rRNA gene sequencing and untargeted metabolomics to assess how the additive of special probiotics’ mixture influenced the swine microbiome and to confirm whether such a proceeding can reduce the risk of some health disturbances’ appearance. ABSTRACT: Complex probiotics are made from various single probiotics mixed in scientific formula. The long-term intake of different probiotics is beneficial to maintain the intestinal microecological balance, inhibiting harmful pathogenic flora and facilitating organism health. Based on the limited research on intestinal flora and related metabolites after the long-term intake of the probiotic complex, in this study, 16S rRNA gene sequencing and untargeted metabolomics were used to further investigate the effects of the probiotic complex on the intestinal flora and metabolome of pigs. The results demonstrated that the content of flora in the intestinal tract or metabolites of pigs varied greatly and was related to cellular metabolic pathways after the long-term feeding of complex probiotics. This study provides a valuable theoretical basis for farmers to raise pigs scientifically and healthily.