Cargando…
Antioxidant, Anti-Inflammatory and Anti-Diabetic Activities of Tectona grandis Methanolic Extracts, Fractions, and Isolated Compounds
Tectona grandis is a traditional Dai medicine plant belonging to the Lamiaceae family, which can be used to treat malaria, inflammation, diabetes, liver disease, bronchitis, tumors, cholelithiasis, jaundice, skin disease and as an anti-helminthic. To find more novel therapeutic agents contained in t...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044725/ https://www.ncbi.nlm.nih.gov/pubmed/36978912 http://dx.doi.org/10.3390/antiox12030664 |
_version_ | 1784913417377677312 |
---|---|
author | Han, Mei Yang, Fengxian Zhang, Kun Ni, Jiyan Zhao, Xia Chen, Xuelin Zhang, Zhennan Wang, Hanlei Lu, Jing Zhang, Yumei |
author_facet | Han, Mei Yang, Fengxian Zhang, Kun Ni, Jiyan Zhao, Xia Chen, Xuelin Zhang, Zhennan Wang, Hanlei Lu, Jing Zhang, Yumei |
author_sort | Han, Mei |
collection | PubMed |
description | Tectona grandis is a traditional Dai medicine plant belonging to the Lamiaceae family, which can be used to treat malaria, inflammation, diabetes, liver disease, bronchitis, tumors, cholelithiasis, jaundice, skin disease and as an anti-helminthic. To find more novel therapeutic agents contained in this medicinal plant, the antioxidant, anti-inflammatory and anti-diabetic activities of T. grandis methanolic extract, fractions and compounds were evaluated. In this study, 26 compounds were isolated from the leaves and branches of T. grandis. Their structures were identified based on extensive spectral experiments, including NMR, ESI-MS and comparison with published spectral data. Among them, compounds 1–2, 4–6, 9–14 and 16–22 were reported for the first time for this plant. The antioxidant activity screening results showed that compounds 5, 15 and 23 had potent antioxidant capacities, with SC(50) values from 0.32 to 9.92 µmol/L, 0.92 to 1.10 mmol Trolox/L and 1.02 to 1.22 mmol Trolox/L for DPPH, ABTS and FRAP, respectively. In addition, their anti-inflammatory effects were investigated by releasing TNF-α, IL-1β and IL-6 through the use of mouse monocytic macrophages (RAW 264.7). Compounds 1, 13, 18 and 23 had the effects of reducing the expression of inflammatory factors. Compounds 13 and 18 were reported for the first time for their anti-inflammatory activities. Furthermore, the methanolic extract (ME), petroleum ether extract (PEE) and EtOAc extract (EAE) of T. grandis showed significant glucose uptake activities; compounds 21 and 23 significantly promoted glucose uptake of 3T3-L1 adipocytes at 40 µM. Meanwhile, compounds 4, 5 and 7 showed significant inhibitory activities against α-glucosidase, with IC(50) values of 14.16 ± 0.34 µmol/L, 19.29 ± 0.26 µmol/L and 3.04 ± 0.08 µmol/L, respectively. Compounds 4 and 5 were reported for the first time for their α-glucosidase inhibitory activities. Our investigation explored the possible therapeutic material basis of T. grandis to prevent oxidative stress and related diseases, especially inflammation and diabetes. |
format | Online Article Text |
id | pubmed-10044725 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100447252023-03-29 Antioxidant, Anti-Inflammatory and Anti-Diabetic Activities of Tectona grandis Methanolic Extracts, Fractions, and Isolated Compounds Han, Mei Yang, Fengxian Zhang, Kun Ni, Jiyan Zhao, Xia Chen, Xuelin Zhang, Zhennan Wang, Hanlei Lu, Jing Zhang, Yumei Antioxidants (Basel) Article Tectona grandis is a traditional Dai medicine plant belonging to the Lamiaceae family, which can be used to treat malaria, inflammation, diabetes, liver disease, bronchitis, tumors, cholelithiasis, jaundice, skin disease and as an anti-helminthic. To find more novel therapeutic agents contained in this medicinal plant, the antioxidant, anti-inflammatory and anti-diabetic activities of T. grandis methanolic extract, fractions and compounds were evaluated. In this study, 26 compounds were isolated from the leaves and branches of T. grandis. Their structures were identified based on extensive spectral experiments, including NMR, ESI-MS and comparison with published spectral data. Among them, compounds 1–2, 4–6, 9–14 and 16–22 were reported for the first time for this plant. The antioxidant activity screening results showed that compounds 5, 15 and 23 had potent antioxidant capacities, with SC(50) values from 0.32 to 9.92 µmol/L, 0.92 to 1.10 mmol Trolox/L and 1.02 to 1.22 mmol Trolox/L for DPPH, ABTS and FRAP, respectively. In addition, their anti-inflammatory effects were investigated by releasing TNF-α, IL-1β and IL-6 through the use of mouse monocytic macrophages (RAW 264.7). Compounds 1, 13, 18 and 23 had the effects of reducing the expression of inflammatory factors. Compounds 13 and 18 were reported for the first time for their anti-inflammatory activities. Furthermore, the methanolic extract (ME), petroleum ether extract (PEE) and EtOAc extract (EAE) of T. grandis showed significant glucose uptake activities; compounds 21 and 23 significantly promoted glucose uptake of 3T3-L1 adipocytes at 40 µM. Meanwhile, compounds 4, 5 and 7 showed significant inhibitory activities against α-glucosidase, with IC(50) values of 14.16 ± 0.34 µmol/L, 19.29 ± 0.26 µmol/L and 3.04 ± 0.08 µmol/L, respectively. Compounds 4 and 5 were reported for the first time for their α-glucosidase inhibitory activities. Our investigation explored the possible therapeutic material basis of T. grandis to prevent oxidative stress and related diseases, especially inflammation and diabetes. MDPI 2023-03-08 /pmc/articles/PMC10044725/ /pubmed/36978912 http://dx.doi.org/10.3390/antiox12030664 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Han, Mei Yang, Fengxian Zhang, Kun Ni, Jiyan Zhao, Xia Chen, Xuelin Zhang, Zhennan Wang, Hanlei Lu, Jing Zhang, Yumei Antioxidant, Anti-Inflammatory and Anti-Diabetic Activities of Tectona grandis Methanolic Extracts, Fractions, and Isolated Compounds |
title | Antioxidant, Anti-Inflammatory and Anti-Diabetic Activities of Tectona grandis Methanolic Extracts, Fractions, and Isolated Compounds |
title_full | Antioxidant, Anti-Inflammatory and Anti-Diabetic Activities of Tectona grandis Methanolic Extracts, Fractions, and Isolated Compounds |
title_fullStr | Antioxidant, Anti-Inflammatory and Anti-Diabetic Activities of Tectona grandis Methanolic Extracts, Fractions, and Isolated Compounds |
title_full_unstemmed | Antioxidant, Anti-Inflammatory and Anti-Diabetic Activities of Tectona grandis Methanolic Extracts, Fractions, and Isolated Compounds |
title_short | Antioxidant, Anti-Inflammatory and Anti-Diabetic Activities of Tectona grandis Methanolic Extracts, Fractions, and Isolated Compounds |
title_sort | antioxidant, anti-inflammatory and anti-diabetic activities of tectona grandis methanolic extracts, fractions, and isolated compounds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044725/ https://www.ncbi.nlm.nih.gov/pubmed/36978912 http://dx.doi.org/10.3390/antiox12030664 |
work_keys_str_mv | AT hanmei antioxidantantiinflammatoryandantidiabeticactivitiesoftectonagrandismethanolicextractsfractionsandisolatedcompounds AT yangfengxian antioxidantantiinflammatoryandantidiabeticactivitiesoftectonagrandismethanolicextractsfractionsandisolatedcompounds AT zhangkun antioxidantantiinflammatoryandantidiabeticactivitiesoftectonagrandismethanolicextractsfractionsandisolatedcompounds AT nijiyan antioxidantantiinflammatoryandantidiabeticactivitiesoftectonagrandismethanolicextractsfractionsandisolatedcompounds AT zhaoxia antioxidantantiinflammatoryandantidiabeticactivitiesoftectonagrandismethanolicextractsfractionsandisolatedcompounds AT chenxuelin antioxidantantiinflammatoryandantidiabeticactivitiesoftectonagrandismethanolicextractsfractionsandisolatedcompounds AT zhangzhennan antioxidantantiinflammatoryandantidiabeticactivitiesoftectonagrandismethanolicextractsfractionsandisolatedcompounds AT wanghanlei antioxidantantiinflammatoryandantidiabeticactivitiesoftectonagrandismethanolicextractsfractionsandisolatedcompounds AT lujing antioxidantantiinflammatoryandantidiabeticactivitiesoftectonagrandismethanolicextractsfractionsandisolatedcompounds AT zhangyumei antioxidantantiinflammatoryandantidiabeticactivitiesoftectonagrandismethanolicextractsfractionsandisolatedcompounds |