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Repurposing Benztropine, Natamycin, and Nitazoxanide Using Drug Combination and Characterization of Gastric Cancer Cell Lines
Gastric cancer (GC) ranked as the fifth most incident cancer in 2020 and the third leading cause of cancer mortality. Surgical prevention and radio/chemotherapy are the main approaches used in GC treatment, and there is an urgent need to explore and discover innovative and effective drugs to better...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044866/ https://www.ncbi.nlm.nih.gov/pubmed/36979779 http://dx.doi.org/10.3390/biomedicines11030799 |
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author | Ribeiro, Eduarda Araújo, Diana Pereira, Mariana Lopes, Bruna Sousa, Patrícia Sousa, Ana Catarina Coelho, André Rêma, Alexandra Alvites, Rui Faria, Fátima Oliveira, Cláudia Porto, Beatriz Maurício, Ana Colette Amorim, Irina Vale, Nuno |
author_facet | Ribeiro, Eduarda Araújo, Diana Pereira, Mariana Lopes, Bruna Sousa, Patrícia Sousa, Ana Catarina Coelho, André Rêma, Alexandra Alvites, Rui Faria, Fátima Oliveira, Cláudia Porto, Beatriz Maurício, Ana Colette Amorim, Irina Vale, Nuno |
author_sort | Ribeiro, Eduarda |
collection | PubMed |
description | Gastric cancer (GC) ranked as the fifth most incident cancer in 2020 and the third leading cause of cancer mortality. Surgical prevention and radio/chemotherapy are the main approaches used in GC treatment, and there is an urgent need to explore and discover innovative and effective drugs to better treat this disease. A new strategy arises with the use of repurposed drugs. Drug repurposing coupled with drug combination schemes has been gaining interest in the scientific community. The main objective of this project was to evaluate the therapeutic effects of alternative drugs in GC. For that, three GC cell lines (AGS, MKN28, and MKN45) were used and characterized. Cell viability assays were performed with the reference drug 5-fluororacil (5-FU) and three repurposed drugs: natamycin, nitazoxanide, and benztropine. Nitazoxanide displayed the best results, being active in all GC cells. Further, 5-FU and nitazoxanide in combination were tested in MKN28 GC cells, and the results obtained showed that nitazoxanide alone was the most promising drug for GC therapy. This work demonstrated that the repurposing of drugs as single agents has the ability to decrease GC cell viability in a concentration-dependent manner. |
format | Online Article Text |
id | pubmed-10044866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100448662023-03-29 Repurposing Benztropine, Natamycin, and Nitazoxanide Using Drug Combination and Characterization of Gastric Cancer Cell Lines Ribeiro, Eduarda Araújo, Diana Pereira, Mariana Lopes, Bruna Sousa, Patrícia Sousa, Ana Catarina Coelho, André Rêma, Alexandra Alvites, Rui Faria, Fátima Oliveira, Cláudia Porto, Beatriz Maurício, Ana Colette Amorim, Irina Vale, Nuno Biomedicines Article Gastric cancer (GC) ranked as the fifth most incident cancer in 2020 and the third leading cause of cancer mortality. Surgical prevention and radio/chemotherapy are the main approaches used in GC treatment, and there is an urgent need to explore and discover innovative and effective drugs to better treat this disease. A new strategy arises with the use of repurposed drugs. Drug repurposing coupled with drug combination schemes has been gaining interest in the scientific community. The main objective of this project was to evaluate the therapeutic effects of alternative drugs in GC. For that, three GC cell lines (AGS, MKN28, and MKN45) were used and characterized. Cell viability assays were performed with the reference drug 5-fluororacil (5-FU) and three repurposed drugs: natamycin, nitazoxanide, and benztropine. Nitazoxanide displayed the best results, being active in all GC cells. Further, 5-FU and nitazoxanide in combination were tested in MKN28 GC cells, and the results obtained showed that nitazoxanide alone was the most promising drug for GC therapy. This work demonstrated that the repurposing of drugs as single agents has the ability to decrease GC cell viability in a concentration-dependent manner. MDPI 2023-03-06 /pmc/articles/PMC10044866/ /pubmed/36979779 http://dx.doi.org/10.3390/biomedicines11030799 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ribeiro, Eduarda Araújo, Diana Pereira, Mariana Lopes, Bruna Sousa, Patrícia Sousa, Ana Catarina Coelho, André Rêma, Alexandra Alvites, Rui Faria, Fátima Oliveira, Cláudia Porto, Beatriz Maurício, Ana Colette Amorim, Irina Vale, Nuno Repurposing Benztropine, Natamycin, and Nitazoxanide Using Drug Combination and Characterization of Gastric Cancer Cell Lines |
title | Repurposing Benztropine, Natamycin, and Nitazoxanide Using Drug Combination and Characterization of Gastric Cancer Cell Lines |
title_full | Repurposing Benztropine, Natamycin, and Nitazoxanide Using Drug Combination and Characterization of Gastric Cancer Cell Lines |
title_fullStr | Repurposing Benztropine, Natamycin, and Nitazoxanide Using Drug Combination and Characterization of Gastric Cancer Cell Lines |
title_full_unstemmed | Repurposing Benztropine, Natamycin, and Nitazoxanide Using Drug Combination and Characterization of Gastric Cancer Cell Lines |
title_short | Repurposing Benztropine, Natamycin, and Nitazoxanide Using Drug Combination and Characterization of Gastric Cancer Cell Lines |
title_sort | repurposing benztropine, natamycin, and nitazoxanide using drug combination and characterization of gastric cancer cell lines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044866/ https://www.ncbi.nlm.nih.gov/pubmed/36979779 http://dx.doi.org/10.3390/biomedicines11030799 |
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