Cargando…
Lactobacillus plantarum Generate Electricity through Flavin Mononucleotide-Mediated Extracellular Electron Transfer to Upregulate Epithelial Type I Collagen Expression and Thereby Promote Microbial Adhesion to Intestine
The mechanism behind how flavin mononucleotide (FMN)-producing bacteria attach to a host intestine remains unclear. In order to address this issue, this study isolated the Gram-positive bacteria Lactobacillus plantarum from Mongolian fermented Airag, named L. plantarum MA. These bacteria were furthe...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045142/ https://www.ncbi.nlm.nih.gov/pubmed/36979656 http://dx.doi.org/10.3390/biomedicines11030677 |
_version_ | 1784913527788535808 |
---|---|
author | Ganzorig, Binderiya Zayabaatar, Enkhbat Pham, Minh Tan Marito, Shinta Huang, Chun-Ming Lee, Yu-Hsiang |
author_facet | Ganzorig, Binderiya Zayabaatar, Enkhbat Pham, Minh Tan Marito, Shinta Huang, Chun-Ming Lee, Yu-Hsiang |
author_sort | Ganzorig, Binderiya |
collection | PubMed |
description | The mechanism behind how flavin mononucleotide (FMN)-producing bacteria attach to a host intestine remains unclear. In order to address this issue, this study isolated the Gram-positive bacteria Lactobacillus plantarum from Mongolian fermented Airag, named L. plantarum MA. These bacteria were further employed as the model microbes, and their electrogenic properties were first identified by their significant expression of type II NADH-quinone oxidoreductase. This study also demonstrated that the electrical activity of L. plantarum MA can be conducted through flavin mononucleotide (FMN)-based extracellular electron transfer, which is highly dependent on the presence of a carbon source in the medium. Our data show that approximately 15 µM of FMN, one of the key electron donors for the generation of electricity, can be produced from L. plantarum MA, as they were cultured in the presence of lactulose for 24 h. We further demonstrated that the electrical activity of L. plantarum MA can promote microbial adhesion and can thus enhance the colonization effectiveness of Caco-2 cells and mouse cecum. Such enhanced adhesiveness was attributed to the increased expression of type I collagens in the intestinal epithelium after treatment with L. plantarum MA. This study reveals the mechanism behind the electrogenic activity of L. plantarum MA and shows how the bacteria utilize electricity to modulate the protein expression of gut tissue for an enhanced adhesion process. |
format | Online Article Text |
id | pubmed-10045142 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100451422023-03-29 Lactobacillus plantarum Generate Electricity through Flavin Mononucleotide-Mediated Extracellular Electron Transfer to Upregulate Epithelial Type I Collagen Expression and Thereby Promote Microbial Adhesion to Intestine Ganzorig, Binderiya Zayabaatar, Enkhbat Pham, Minh Tan Marito, Shinta Huang, Chun-Ming Lee, Yu-Hsiang Biomedicines Article The mechanism behind how flavin mononucleotide (FMN)-producing bacteria attach to a host intestine remains unclear. In order to address this issue, this study isolated the Gram-positive bacteria Lactobacillus plantarum from Mongolian fermented Airag, named L. plantarum MA. These bacteria were further employed as the model microbes, and their electrogenic properties were first identified by their significant expression of type II NADH-quinone oxidoreductase. This study also demonstrated that the electrical activity of L. plantarum MA can be conducted through flavin mononucleotide (FMN)-based extracellular electron transfer, which is highly dependent on the presence of a carbon source in the medium. Our data show that approximately 15 µM of FMN, one of the key electron donors for the generation of electricity, can be produced from L. plantarum MA, as they were cultured in the presence of lactulose for 24 h. We further demonstrated that the electrical activity of L. plantarum MA can promote microbial adhesion and can thus enhance the colonization effectiveness of Caco-2 cells and mouse cecum. Such enhanced adhesiveness was attributed to the increased expression of type I collagens in the intestinal epithelium after treatment with L. plantarum MA. This study reveals the mechanism behind the electrogenic activity of L. plantarum MA and shows how the bacteria utilize electricity to modulate the protein expression of gut tissue for an enhanced adhesion process. MDPI 2023-02-23 /pmc/articles/PMC10045142/ /pubmed/36979656 http://dx.doi.org/10.3390/biomedicines11030677 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ganzorig, Binderiya Zayabaatar, Enkhbat Pham, Minh Tan Marito, Shinta Huang, Chun-Ming Lee, Yu-Hsiang Lactobacillus plantarum Generate Electricity through Flavin Mononucleotide-Mediated Extracellular Electron Transfer to Upregulate Epithelial Type I Collagen Expression and Thereby Promote Microbial Adhesion to Intestine |
title | Lactobacillus plantarum Generate Electricity through Flavin Mononucleotide-Mediated Extracellular Electron Transfer to Upregulate Epithelial Type I Collagen Expression and Thereby Promote Microbial Adhesion to Intestine |
title_full | Lactobacillus plantarum Generate Electricity through Flavin Mononucleotide-Mediated Extracellular Electron Transfer to Upregulate Epithelial Type I Collagen Expression and Thereby Promote Microbial Adhesion to Intestine |
title_fullStr | Lactobacillus plantarum Generate Electricity through Flavin Mononucleotide-Mediated Extracellular Electron Transfer to Upregulate Epithelial Type I Collagen Expression and Thereby Promote Microbial Adhesion to Intestine |
title_full_unstemmed | Lactobacillus plantarum Generate Electricity through Flavin Mononucleotide-Mediated Extracellular Electron Transfer to Upregulate Epithelial Type I Collagen Expression and Thereby Promote Microbial Adhesion to Intestine |
title_short | Lactobacillus plantarum Generate Electricity through Flavin Mononucleotide-Mediated Extracellular Electron Transfer to Upregulate Epithelial Type I Collagen Expression and Thereby Promote Microbial Adhesion to Intestine |
title_sort | lactobacillus plantarum generate electricity through flavin mononucleotide-mediated extracellular electron transfer to upregulate epithelial type i collagen expression and thereby promote microbial adhesion to intestine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045142/ https://www.ncbi.nlm.nih.gov/pubmed/36979656 http://dx.doi.org/10.3390/biomedicines11030677 |
work_keys_str_mv | AT ganzorigbinderiya lactobacillusplantarumgenerateelectricitythroughflavinmononucleotidemediatedextracellularelectrontransfertoupregulateepithelialtypeicollagenexpressionandtherebypromotemicrobialadhesiontointestine AT zayabaatarenkhbat lactobacillusplantarumgenerateelectricitythroughflavinmononucleotidemediatedextracellularelectrontransfertoupregulateepithelialtypeicollagenexpressionandtherebypromotemicrobialadhesiontointestine AT phamminhtan lactobacillusplantarumgenerateelectricitythroughflavinmononucleotidemediatedextracellularelectrontransfertoupregulateepithelialtypeicollagenexpressionandtherebypromotemicrobialadhesiontointestine AT maritoshinta lactobacillusplantarumgenerateelectricitythroughflavinmononucleotidemediatedextracellularelectrontransfertoupregulateepithelialtypeicollagenexpressionandtherebypromotemicrobialadhesiontointestine AT huangchunming lactobacillusplantarumgenerateelectricitythroughflavinmononucleotidemediatedextracellularelectrontransfertoupregulateepithelialtypeicollagenexpressionandtherebypromotemicrobialadhesiontointestine AT leeyuhsiang lactobacillusplantarumgenerateelectricitythroughflavinmononucleotidemediatedextracellularelectrontransfertoupregulateepithelialtypeicollagenexpressionandtherebypromotemicrobialadhesiontointestine |