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Reinforcement of Calcium Phosphate Cement with Hybrid Silk Fibroin/Kappa-Carrageenan Nanofibers

Calcium phosphate cements (CPCs) offer a promising solution for treating bone defects due to their osteoconductive, injectable, biocompatible, and bone replacement properties. However, their brittle nature restricts their utilization to non-load-bearing applications. In this study, the impact of hyb...

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Autores principales: Roshanfar, Fahimeh, Hesaraki, Saeed, Dolatshahi-Pirouz, Alireza, Saeidi, Mohsen, Leal-Marin, Sara, Glasmacher, Birgit, Orive, Gorka, Khan Einipour, Sajjad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045238/
https://www.ncbi.nlm.nih.gov/pubmed/36979830
http://dx.doi.org/10.3390/biomedicines11030850
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author Roshanfar, Fahimeh
Hesaraki, Saeed
Dolatshahi-Pirouz, Alireza
Saeidi, Mohsen
Leal-Marin, Sara
Glasmacher, Birgit
Orive, Gorka
Khan Einipour, Sajjad
author_facet Roshanfar, Fahimeh
Hesaraki, Saeed
Dolatshahi-Pirouz, Alireza
Saeidi, Mohsen
Leal-Marin, Sara
Glasmacher, Birgit
Orive, Gorka
Khan Einipour, Sajjad
author_sort Roshanfar, Fahimeh
collection PubMed
description Calcium phosphate cements (CPCs) offer a promising solution for treating bone defects due to their osteoconductive, injectable, biocompatible, and bone replacement properties. However, their brittle nature restricts their utilization to non-load-bearing applications. In this study, the impact of hybrid silk fibroin (SF) and kappa-carrageenan (k-CG) nanofibers as reinforcements in CPC was investigated. The CPC composite was fabricated by incorporating electrospun nanofibers in 1, 3, and 5% volume fractions. The morphology, mineralization, mechanical properties, setting time, injectability, cell adhesion, and mineralization of the CPC composites were analyzed. The results demonstrated that the addition of the nanofibers improved the CPC mixture, leading to an increase in compressive strength (14.8 ± 0.3 MPa compared to 8.1 ± 0.4 MPa of the unreinforced CPC). Similar improvements were seen in the bending strength and work fracture (WOF). The MC3T3-E1 cell culture experiments indicated that cells attached well to the surfaces of all cement samples and tended to join their adjacent cells. Additionally, the CPC composites showed higher cell mineralization after a culture period of 14 days, indicating that the SF/k-CG combination has potential for applications as a CPC reinforcement and bone cell regeneration promoter.
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spelling pubmed-100452382023-03-29 Reinforcement of Calcium Phosphate Cement with Hybrid Silk Fibroin/Kappa-Carrageenan Nanofibers Roshanfar, Fahimeh Hesaraki, Saeed Dolatshahi-Pirouz, Alireza Saeidi, Mohsen Leal-Marin, Sara Glasmacher, Birgit Orive, Gorka Khan Einipour, Sajjad Biomedicines Article Calcium phosphate cements (CPCs) offer a promising solution for treating bone defects due to their osteoconductive, injectable, biocompatible, and bone replacement properties. However, their brittle nature restricts their utilization to non-load-bearing applications. In this study, the impact of hybrid silk fibroin (SF) and kappa-carrageenan (k-CG) nanofibers as reinforcements in CPC was investigated. The CPC composite was fabricated by incorporating electrospun nanofibers in 1, 3, and 5% volume fractions. The morphology, mineralization, mechanical properties, setting time, injectability, cell adhesion, and mineralization of the CPC composites were analyzed. The results demonstrated that the addition of the nanofibers improved the CPC mixture, leading to an increase in compressive strength (14.8 ± 0.3 MPa compared to 8.1 ± 0.4 MPa of the unreinforced CPC). Similar improvements were seen in the bending strength and work fracture (WOF). The MC3T3-E1 cell culture experiments indicated that cells attached well to the surfaces of all cement samples and tended to join their adjacent cells. Additionally, the CPC composites showed higher cell mineralization after a culture period of 14 days, indicating that the SF/k-CG combination has potential for applications as a CPC reinforcement and bone cell regeneration promoter. MDPI 2023-03-10 /pmc/articles/PMC10045238/ /pubmed/36979830 http://dx.doi.org/10.3390/biomedicines11030850 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Roshanfar, Fahimeh
Hesaraki, Saeed
Dolatshahi-Pirouz, Alireza
Saeidi, Mohsen
Leal-Marin, Sara
Glasmacher, Birgit
Orive, Gorka
Khan Einipour, Sajjad
Reinforcement of Calcium Phosphate Cement with Hybrid Silk Fibroin/Kappa-Carrageenan Nanofibers
title Reinforcement of Calcium Phosphate Cement with Hybrid Silk Fibroin/Kappa-Carrageenan Nanofibers
title_full Reinforcement of Calcium Phosphate Cement with Hybrid Silk Fibroin/Kappa-Carrageenan Nanofibers
title_fullStr Reinforcement of Calcium Phosphate Cement with Hybrid Silk Fibroin/Kappa-Carrageenan Nanofibers
title_full_unstemmed Reinforcement of Calcium Phosphate Cement with Hybrid Silk Fibroin/Kappa-Carrageenan Nanofibers
title_short Reinforcement of Calcium Phosphate Cement with Hybrid Silk Fibroin/Kappa-Carrageenan Nanofibers
title_sort reinforcement of calcium phosphate cement with hybrid silk fibroin/kappa-carrageenan nanofibers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045238/
https://www.ncbi.nlm.nih.gov/pubmed/36979830
http://dx.doi.org/10.3390/biomedicines11030850
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