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Experimentally Induced Burns in Rats Treated with Innovative Polymeric Films Type Therapies

Considering that microbial resistance to antibiotics is becoming an increasingly widespread problem, burn management, which usually includes the use of topical antimicrobial dressings, is still facing difficulties regarding their efficiency to ensure rapid healing. In this context, the main objectiv...

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Autores principales: Grosu, Oxana-Madalina, Dragostin, Oana-Maria, Gardikiotis, Ioannis, Chitescu, Carmen Lidia, Lisa, Elena Lacramioara, Zamfir, Alexandra-Simona, Confederat, Luminita, Dragostin, Ionut, Dragan, Maria, Stan, Catalina Daniela, Zamfir, Carmen-Lacramioara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045338/
https://www.ncbi.nlm.nih.gov/pubmed/36979831
http://dx.doi.org/10.3390/biomedicines11030852
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author Grosu, Oxana-Madalina
Dragostin, Oana-Maria
Gardikiotis, Ioannis
Chitescu, Carmen Lidia
Lisa, Elena Lacramioara
Zamfir, Alexandra-Simona
Confederat, Luminita
Dragostin, Ionut
Dragan, Maria
Stan, Catalina Daniela
Zamfir, Carmen-Lacramioara
author_facet Grosu, Oxana-Madalina
Dragostin, Oana-Maria
Gardikiotis, Ioannis
Chitescu, Carmen Lidia
Lisa, Elena Lacramioara
Zamfir, Alexandra-Simona
Confederat, Luminita
Dragostin, Ionut
Dragan, Maria
Stan, Catalina Daniela
Zamfir, Carmen-Lacramioara
author_sort Grosu, Oxana-Madalina
collection PubMed
description Considering that microbial resistance to antibiotics is becoming an increasingly widespread problem, burn management, which usually includes the use of topical antimicrobial dressings, is still facing difficulties regarding their efficiency to ensure rapid healing. In this context, the main objective of this research is to include new oxytetracycline derivatives in polymeric-film-type dressings for the treatment of wounds caused by experimentally induced burns in rats. The structural and physico-chemical properties of synthesized oxytetracycline derivatives and the corresponding membranes were analyzed by FT-IR and MS spectroscopy, swelling ability and biodegradation capacity. In vitro antimicrobial activity using Gram-positive and Gram-negative bacterial strains and pathogenic yeasts, along with an in vivo study of a burn wound model induced in Wistar rats, was also analyzed. The newly obtained polymeric films, namely chitosan-oxytetracycline derivative membranes, showed good antimicrobial activity noticed in the tested strains, a membrane swelling ratio (MSR) of up to 1578% in acidic conditions and a biodegradation rate of up to 15.7% on day 7 of testing, which are important required characteristics for the tissue regeneration process, after the production of a burn. The in vivo study proved that chitosan-derived oxytetracycline membranes showed also improved healing effects which contributes to supporting the idea of using them for the treatment of wounds caused by burns.
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spelling pubmed-100453382023-03-29 Experimentally Induced Burns in Rats Treated with Innovative Polymeric Films Type Therapies Grosu, Oxana-Madalina Dragostin, Oana-Maria Gardikiotis, Ioannis Chitescu, Carmen Lidia Lisa, Elena Lacramioara Zamfir, Alexandra-Simona Confederat, Luminita Dragostin, Ionut Dragan, Maria Stan, Catalina Daniela Zamfir, Carmen-Lacramioara Biomedicines Article Considering that microbial resistance to antibiotics is becoming an increasingly widespread problem, burn management, which usually includes the use of topical antimicrobial dressings, is still facing difficulties regarding their efficiency to ensure rapid healing. In this context, the main objective of this research is to include new oxytetracycline derivatives in polymeric-film-type dressings for the treatment of wounds caused by experimentally induced burns in rats. The structural and physico-chemical properties of synthesized oxytetracycline derivatives and the corresponding membranes were analyzed by FT-IR and MS spectroscopy, swelling ability and biodegradation capacity. In vitro antimicrobial activity using Gram-positive and Gram-negative bacterial strains and pathogenic yeasts, along with an in vivo study of a burn wound model induced in Wistar rats, was also analyzed. The newly obtained polymeric films, namely chitosan-oxytetracycline derivative membranes, showed good antimicrobial activity noticed in the tested strains, a membrane swelling ratio (MSR) of up to 1578% in acidic conditions and a biodegradation rate of up to 15.7% on day 7 of testing, which are important required characteristics for the tissue regeneration process, after the production of a burn. The in vivo study proved that chitosan-derived oxytetracycline membranes showed also improved healing effects which contributes to supporting the idea of using them for the treatment of wounds caused by burns. MDPI 2023-03-10 /pmc/articles/PMC10045338/ /pubmed/36979831 http://dx.doi.org/10.3390/biomedicines11030852 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grosu, Oxana-Madalina
Dragostin, Oana-Maria
Gardikiotis, Ioannis
Chitescu, Carmen Lidia
Lisa, Elena Lacramioara
Zamfir, Alexandra-Simona
Confederat, Luminita
Dragostin, Ionut
Dragan, Maria
Stan, Catalina Daniela
Zamfir, Carmen-Lacramioara
Experimentally Induced Burns in Rats Treated with Innovative Polymeric Films Type Therapies
title Experimentally Induced Burns in Rats Treated with Innovative Polymeric Films Type Therapies
title_full Experimentally Induced Burns in Rats Treated with Innovative Polymeric Films Type Therapies
title_fullStr Experimentally Induced Burns in Rats Treated with Innovative Polymeric Films Type Therapies
title_full_unstemmed Experimentally Induced Burns in Rats Treated with Innovative Polymeric Films Type Therapies
title_short Experimentally Induced Burns in Rats Treated with Innovative Polymeric Films Type Therapies
title_sort experimentally induced burns in rats treated with innovative polymeric films type therapies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045338/
https://www.ncbi.nlm.nih.gov/pubmed/36979831
http://dx.doi.org/10.3390/biomedicines11030852
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