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Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells

The effect of extracellular matrix (ECM) stiffness on embryonic trophoblast cells invasion during mammalian embryo implantation remains largely unknown. In this study, we investigated the effects of ECM stiffness on various aspects of human trophoblast cell behaviors during cell–ECM interactions. Th...

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Detalles Bibliográficos
Autores principales: Cao, Jialing, Li, Hangyu, Tang, Hongyan, Gu, Xuenan, Wang, Yan, Guan, Dongshi, Du, Jing, Fan, Yubo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045595/
https://www.ncbi.nlm.nih.gov/pubmed/36978775
http://dx.doi.org/10.3390/bioengineering10030384
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author Cao, Jialing
Li, Hangyu
Tang, Hongyan
Gu, Xuenan
Wang, Yan
Guan, Dongshi
Du, Jing
Fan, Yubo
author_facet Cao, Jialing
Li, Hangyu
Tang, Hongyan
Gu, Xuenan
Wang, Yan
Guan, Dongshi
Du, Jing
Fan, Yubo
author_sort Cao, Jialing
collection PubMed
description The effect of extracellular matrix (ECM) stiffness on embryonic trophoblast cells invasion during mammalian embryo implantation remains largely unknown. In this study, we investigated the effects of ECM stiffness on various aspects of human trophoblast cell behaviors during cell–ECM interactions. The mechanical microenvironment of the uterus was simulated by fabricating polyacrylamide (PA) hydrogels with different levels of stiffness. The human choriocarcinoma (JAR) cell lineage was used as the trophoblast model. We found that the spreading area of JAR cells, the formation of focal adhesions, and the polymerization of the F-actin cytoskeleton were all facilitated with increased ECM stiffness. Significantly, JAR cells also exhibited durotactic behavior on ECM with a gradient stiffness. Meanwhile, stiffness of the ECM affects the invasion of multicellular JAR spheroids. These results demonstrated that human trophoblast cells are mechanically sensitive, while the mechanical properties of the uterine microenvironment could play an important role in the implantation process.
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spelling pubmed-100455952023-03-29 Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells Cao, Jialing Li, Hangyu Tang, Hongyan Gu, Xuenan Wang, Yan Guan, Dongshi Du, Jing Fan, Yubo Bioengineering (Basel) Article The effect of extracellular matrix (ECM) stiffness on embryonic trophoblast cells invasion during mammalian embryo implantation remains largely unknown. In this study, we investigated the effects of ECM stiffness on various aspects of human trophoblast cell behaviors during cell–ECM interactions. The mechanical microenvironment of the uterus was simulated by fabricating polyacrylamide (PA) hydrogels with different levels of stiffness. The human choriocarcinoma (JAR) cell lineage was used as the trophoblast model. We found that the spreading area of JAR cells, the formation of focal adhesions, and the polymerization of the F-actin cytoskeleton were all facilitated with increased ECM stiffness. Significantly, JAR cells also exhibited durotactic behavior on ECM with a gradient stiffness. Meanwhile, stiffness of the ECM affects the invasion of multicellular JAR spheroids. These results demonstrated that human trophoblast cells are mechanically sensitive, while the mechanical properties of the uterine microenvironment could play an important role in the implantation process. MDPI 2023-03-21 /pmc/articles/PMC10045595/ /pubmed/36978775 http://dx.doi.org/10.3390/bioengineering10030384 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cao, Jialing
Li, Hangyu
Tang, Hongyan
Gu, Xuenan
Wang, Yan
Guan, Dongshi
Du, Jing
Fan, Yubo
Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells
title Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells
title_full Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells
title_fullStr Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells
title_full_unstemmed Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells
title_short Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells
title_sort stiff extracellular matrix promotes invasive behaviors of trophoblast cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045595/
https://www.ncbi.nlm.nih.gov/pubmed/36978775
http://dx.doi.org/10.3390/bioengineering10030384
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