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Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities
Isoquercetin (ISQ) is reported to be a powerful antioxidant with extremely high bioavailability and structural stability compared to aglycone quercetin. Despite this, it is not well studied due to the limited methods for its extraction. With the growing interest in the research and analysis of ISQ-r...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045738/ https://www.ncbi.nlm.nih.gov/pubmed/36978973 http://dx.doi.org/10.3390/antiox12030725 |
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author | El Maaiden, Ezzouhra Qarah, Nagib Ezzariai, Amine Mazar, Adil Nasser, Boubker Moustaid, Khadija Boukcim, Hassan Hirich, Abdelaziz Kouisni, Lamfeddal El Kharrassi, Youssef |
author_facet | El Maaiden, Ezzouhra Qarah, Nagib Ezzariai, Amine Mazar, Adil Nasser, Boubker Moustaid, Khadija Boukcim, Hassan Hirich, Abdelaziz Kouisni, Lamfeddal El Kharrassi, Youssef |
author_sort | El Maaiden, Ezzouhra |
collection | PubMed |
description | Isoquercetin (ISQ) is reported to be a powerful antioxidant with extremely high bioavailability and structural stability compared to aglycone quercetin. Despite this, it is not well studied due to the limited methods for its extraction. With the growing interest in the research and analysis of ISQ-rich herbs, there is a need to optimize an efficient and rapid method for their extraction. In the present study, the ultrasound-assisted extraction of ISQ from Ephedra alata Decne was optimized by a response surface methodology (RSM) using high-performance liquid chromatography as a separation method. The best possible ranges for extraction time (10–30 min), temperature (50–70 °C), ultrasonic power (60–90 W), solvent-to-solid ratio (50–70 mL/g), and ethanol concentration (50–70%) were determined using a single factor analysis. Subsequently, an optimization of the extraction conditions was performed with RSM using the Box–Behnken design. An ultrasonication time of 10 min, a temperature of 60 °C, a power of 75 W, a solvent-to-solid ratio of 60 mL/g, and an ethanol concentration of 70% were determined to be the optimal conditions for the highest recovery of isoquercetin (1033.96 ± 3.28 µg/g). Furthermore, E. alata powder morphology (using a scanning electron microscope), antioxidant activities, and the inhibition potential of key enzymes involved in skin aging (elastase and collagenase), hyperpigmentation (tyrosinase), diabetes (α-amylase), inflammation (hyaluronidase), and neurodegenerative disorders (cholinesterase) were determined and compared with those using the Soxhlet method. This study established a highly efficient method for ISQ extraction and suggested several potential applications of ISQ in the pharmaceutical and cosmetics industries. |
format | Online Article Text |
id | pubmed-10045738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100457382023-03-29 Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities El Maaiden, Ezzouhra Qarah, Nagib Ezzariai, Amine Mazar, Adil Nasser, Boubker Moustaid, Khadija Boukcim, Hassan Hirich, Abdelaziz Kouisni, Lamfeddal El Kharrassi, Youssef Antioxidants (Basel) Article Isoquercetin (ISQ) is reported to be a powerful antioxidant with extremely high bioavailability and structural stability compared to aglycone quercetin. Despite this, it is not well studied due to the limited methods for its extraction. With the growing interest in the research and analysis of ISQ-rich herbs, there is a need to optimize an efficient and rapid method for their extraction. In the present study, the ultrasound-assisted extraction of ISQ from Ephedra alata Decne was optimized by a response surface methodology (RSM) using high-performance liquid chromatography as a separation method. The best possible ranges for extraction time (10–30 min), temperature (50–70 °C), ultrasonic power (60–90 W), solvent-to-solid ratio (50–70 mL/g), and ethanol concentration (50–70%) were determined using a single factor analysis. Subsequently, an optimization of the extraction conditions was performed with RSM using the Box–Behnken design. An ultrasonication time of 10 min, a temperature of 60 °C, a power of 75 W, a solvent-to-solid ratio of 60 mL/g, and an ethanol concentration of 70% were determined to be the optimal conditions for the highest recovery of isoquercetin (1033.96 ± 3.28 µg/g). Furthermore, E. alata powder morphology (using a scanning electron microscope), antioxidant activities, and the inhibition potential of key enzymes involved in skin aging (elastase and collagenase), hyperpigmentation (tyrosinase), diabetes (α-amylase), inflammation (hyaluronidase), and neurodegenerative disorders (cholinesterase) were determined and compared with those using the Soxhlet method. This study established a highly efficient method for ISQ extraction and suggested several potential applications of ISQ in the pharmaceutical and cosmetics industries. MDPI 2023-03-15 /pmc/articles/PMC10045738/ /pubmed/36978973 http://dx.doi.org/10.3390/antiox12030725 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article El Maaiden, Ezzouhra Qarah, Nagib Ezzariai, Amine Mazar, Adil Nasser, Boubker Moustaid, Khadija Boukcim, Hassan Hirich, Abdelaziz Kouisni, Lamfeddal El Kharrassi, Youssef Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities |
title | Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities |
title_full | Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities |
title_fullStr | Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities |
title_full_unstemmed | Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities |
title_short | Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities |
title_sort | ultrasound-assisted extraction of isoquercetin from ephedra alata (decne): optimization using response surface methodology and in vitro bioactivities |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045738/ https://www.ncbi.nlm.nih.gov/pubmed/36978973 http://dx.doi.org/10.3390/antiox12030725 |
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