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Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities

Isoquercetin (ISQ) is reported to be a powerful antioxidant with extremely high bioavailability and structural stability compared to aglycone quercetin. Despite this, it is not well studied due to the limited methods for its extraction. With the growing interest in the research and analysis of ISQ-r...

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Autores principales: El Maaiden, Ezzouhra, Qarah, Nagib, Ezzariai, Amine, Mazar, Adil, Nasser, Boubker, Moustaid, Khadija, Boukcim, Hassan, Hirich, Abdelaziz, Kouisni, Lamfeddal, El Kharrassi, Youssef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045738/
https://www.ncbi.nlm.nih.gov/pubmed/36978973
http://dx.doi.org/10.3390/antiox12030725
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author El Maaiden, Ezzouhra
Qarah, Nagib
Ezzariai, Amine
Mazar, Adil
Nasser, Boubker
Moustaid, Khadija
Boukcim, Hassan
Hirich, Abdelaziz
Kouisni, Lamfeddal
El Kharrassi, Youssef
author_facet El Maaiden, Ezzouhra
Qarah, Nagib
Ezzariai, Amine
Mazar, Adil
Nasser, Boubker
Moustaid, Khadija
Boukcim, Hassan
Hirich, Abdelaziz
Kouisni, Lamfeddal
El Kharrassi, Youssef
author_sort El Maaiden, Ezzouhra
collection PubMed
description Isoquercetin (ISQ) is reported to be a powerful antioxidant with extremely high bioavailability and structural stability compared to aglycone quercetin. Despite this, it is not well studied due to the limited methods for its extraction. With the growing interest in the research and analysis of ISQ-rich herbs, there is a need to optimize an efficient and rapid method for their extraction. In the present study, the ultrasound-assisted extraction of ISQ from Ephedra alata Decne was optimized by a response surface methodology (RSM) using high-performance liquid chromatography as a separation method. The best possible ranges for extraction time (10–30 min), temperature (50–70 °C), ultrasonic power (60–90 W), solvent-to-solid ratio (50–70 mL/g), and ethanol concentration (50–70%) were determined using a single factor analysis. Subsequently, an optimization of the extraction conditions was performed with RSM using the Box–Behnken design. An ultrasonication time of 10 min, a temperature of 60 °C, a power of 75 W, a solvent-to-solid ratio of 60 mL/g, and an ethanol concentration of 70% were determined to be the optimal conditions for the highest recovery of isoquercetin (1033.96 ± 3.28 µg/g). Furthermore, E. alata powder morphology (using a scanning electron microscope), antioxidant activities, and the inhibition potential of key enzymes involved in skin aging (elastase and collagenase), hyperpigmentation (tyrosinase), diabetes (α-amylase), inflammation (hyaluronidase), and neurodegenerative disorders (cholinesterase) were determined and compared with those using the Soxhlet method. This study established a highly efficient method for ISQ extraction and suggested several potential applications of ISQ in the pharmaceutical and cosmetics industries.
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spelling pubmed-100457382023-03-29 Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities El Maaiden, Ezzouhra Qarah, Nagib Ezzariai, Amine Mazar, Adil Nasser, Boubker Moustaid, Khadija Boukcim, Hassan Hirich, Abdelaziz Kouisni, Lamfeddal El Kharrassi, Youssef Antioxidants (Basel) Article Isoquercetin (ISQ) is reported to be a powerful antioxidant with extremely high bioavailability and structural stability compared to aglycone quercetin. Despite this, it is not well studied due to the limited methods for its extraction. With the growing interest in the research and analysis of ISQ-rich herbs, there is a need to optimize an efficient and rapid method for their extraction. In the present study, the ultrasound-assisted extraction of ISQ from Ephedra alata Decne was optimized by a response surface methodology (RSM) using high-performance liquid chromatography as a separation method. The best possible ranges for extraction time (10–30 min), temperature (50–70 °C), ultrasonic power (60–90 W), solvent-to-solid ratio (50–70 mL/g), and ethanol concentration (50–70%) were determined using a single factor analysis. Subsequently, an optimization of the extraction conditions was performed with RSM using the Box–Behnken design. An ultrasonication time of 10 min, a temperature of 60 °C, a power of 75 W, a solvent-to-solid ratio of 60 mL/g, and an ethanol concentration of 70% were determined to be the optimal conditions for the highest recovery of isoquercetin (1033.96 ± 3.28 µg/g). Furthermore, E. alata powder morphology (using a scanning electron microscope), antioxidant activities, and the inhibition potential of key enzymes involved in skin aging (elastase and collagenase), hyperpigmentation (tyrosinase), diabetes (α-amylase), inflammation (hyaluronidase), and neurodegenerative disorders (cholinesterase) were determined and compared with those using the Soxhlet method. This study established a highly efficient method for ISQ extraction and suggested several potential applications of ISQ in the pharmaceutical and cosmetics industries. MDPI 2023-03-15 /pmc/articles/PMC10045738/ /pubmed/36978973 http://dx.doi.org/10.3390/antiox12030725 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
El Maaiden, Ezzouhra
Qarah, Nagib
Ezzariai, Amine
Mazar, Adil
Nasser, Boubker
Moustaid, Khadija
Boukcim, Hassan
Hirich, Abdelaziz
Kouisni, Lamfeddal
El Kharrassi, Youssef
Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities
title Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities
title_full Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities
title_fullStr Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities
title_full_unstemmed Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities
title_short Ultrasound-Assisted Extraction of Isoquercetin from Ephedra alata (Decne): Optimization Using Response Surface Methodology and In Vitro Bioactivities
title_sort ultrasound-assisted extraction of isoquercetin from ephedra alata (decne): optimization using response surface methodology and in vitro bioactivities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045738/
https://www.ncbi.nlm.nih.gov/pubmed/36978973
http://dx.doi.org/10.3390/antiox12030725
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