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GmMPK6 Positively Regulates Salt Tolerance through Induction of GmRbohI1 in Soybean
Salt stress is a critical environmental stress that impairs plant growth and development, especially in crop productivity; therefore, understanding the salt response in plants is the basis for their development of salt tolerance. Under salinity, soybean mitogen-activated protein kinase 6 (GmMPK6) is...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045776/ https://www.ncbi.nlm.nih.gov/pubmed/36978849 http://dx.doi.org/10.3390/antiox12030601 |
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author | Son, Seungmin Kim, Jitae An, Chung Sun Kim, Song Lim Lee, Hyoungseok Im, Jong Hee |
author_facet | Son, Seungmin Kim, Jitae An, Chung Sun Kim, Song Lim Lee, Hyoungseok Im, Jong Hee |
author_sort | Son, Seungmin |
collection | PubMed |
description | Salt stress is a critical environmental stress that impairs plant growth and development, especially in crop productivity; therefore, understanding the salt response in plants is the basis for their development of salt tolerance. Under salinity, soybean mitogen-activated protein kinase 6 (GmMPK6) is activated and positively regulates reactive oxygen species (ROS) generation. However, it is not yet elucidated how GmMPK6 regulates ROS generation and its role in salt tolerance. Here, we show that GmMPK6, solely activated in NaCl treatment, and gene expression of GmRbohI1 was not only reduced by MPK inhibitor SB202190 in NaCl treatment, but also increased in a GMKK1-expressing protoplast. Furthermore, SB202190 and the NADPH-oxidase inhibitor, diphenyleneiodonium chloride, increased susceptibility to salt stress. The expression of GmRD19A was induced by NaCl treatment, but this expression was compromised by SB202190. Consequently, we revealed that GmMPK6 induces ROS generation through the transcriptional regulation of GmRbohI1 and increases salt tolerance in soybean. |
format | Online Article Text |
id | pubmed-10045776 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100457762023-03-29 GmMPK6 Positively Regulates Salt Tolerance through Induction of GmRbohI1 in Soybean Son, Seungmin Kim, Jitae An, Chung Sun Kim, Song Lim Lee, Hyoungseok Im, Jong Hee Antioxidants (Basel) Communication Salt stress is a critical environmental stress that impairs plant growth and development, especially in crop productivity; therefore, understanding the salt response in plants is the basis for their development of salt tolerance. Under salinity, soybean mitogen-activated protein kinase 6 (GmMPK6) is activated and positively regulates reactive oxygen species (ROS) generation. However, it is not yet elucidated how GmMPK6 regulates ROS generation and its role in salt tolerance. Here, we show that GmMPK6, solely activated in NaCl treatment, and gene expression of GmRbohI1 was not only reduced by MPK inhibitor SB202190 in NaCl treatment, but also increased in a GMKK1-expressing protoplast. Furthermore, SB202190 and the NADPH-oxidase inhibitor, diphenyleneiodonium chloride, increased susceptibility to salt stress. The expression of GmRD19A was induced by NaCl treatment, but this expression was compromised by SB202190. Consequently, we revealed that GmMPK6 induces ROS generation through the transcriptional regulation of GmRbohI1 and increases salt tolerance in soybean. MDPI 2023-02-28 /pmc/articles/PMC10045776/ /pubmed/36978849 http://dx.doi.org/10.3390/antiox12030601 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Son, Seungmin Kim, Jitae An, Chung Sun Kim, Song Lim Lee, Hyoungseok Im, Jong Hee GmMPK6 Positively Regulates Salt Tolerance through Induction of GmRbohI1 in Soybean |
title | GmMPK6 Positively Regulates Salt Tolerance through Induction of GmRbohI1 in Soybean |
title_full | GmMPK6 Positively Regulates Salt Tolerance through Induction of GmRbohI1 in Soybean |
title_fullStr | GmMPK6 Positively Regulates Salt Tolerance through Induction of GmRbohI1 in Soybean |
title_full_unstemmed | GmMPK6 Positively Regulates Salt Tolerance through Induction of GmRbohI1 in Soybean |
title_short | GmMPK6 Positively Regulates Salt Tolerance through Induction of GmRbohI1 in Soybean |
title_sort | gmmpk6 positively regulates salt tolerance through induction of gmrbohi1 in soybean |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045776/ https://www.ncbi.nlm.nih.gov/pubmed/36978849 http://dx.doi.org/10.3390/antiox12030601 |
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