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Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE)

The determination of antidepressant drugs and their metabolites in the body, mainly in the blood, allows for the monitoring of drug levels and their metabolism, helps identify drug interactions, and reduces the likelihood of increased side effects. Due to numerous inconveniences associated with coll...

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Autores principales: Dziurkowska, Ewelina, Wesolowski, Marek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045804/
https://www.ncbi.nlm.nih.gov/pubmed/36979687
http://dx.doi.org/10.3390/biomedicines11030708
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author Dziurkowska, Ewelina
Wesolowski, Marek
author_facet Dziurkowska, Ewelina
Wesolowski, Marek
author_sort Dziurkowska, Ewelina
collection PubMed
description The determination of antidepressant drugs and their metabolites in the body, mainly in the blood, allows for the monitoring of drug levels and their metabolism, helps identify drug interactions, and reduces the likelihood of increased side effects. Due to numerous inconveniences associated with collecting blood in patients, therapeutic drug monitoring (TDM) based on saliva sampling could significantly improve patient comfort. Therefore, the aim of this study was to develop a method for the simultaneous determination of selected antidepressants (amitriptyline, mianserin, duloxetine, mirtazapine, sertraline, citalopram, and venlafaxine) and their metabolites (N-desmethylmirtazapine, norsertraline, N-desmethylcitalopram, O-desmethylvenlafaxine) in human saliva using supported liquid extraction (SLE). Chlordiazepoxide was used as an internal standard. UHPLC coupled with DAD detection was used for the determinations. The proposed method was validated by determining its linearity for saliva concentrations in the range 10–1000 ng/mL. For all the analyzed compounds, a linear relationship between the analytical signal and analyte concentration was obtained (R(2) > 0.99), with the intra- and inter-day precisions expressed as a coefficient of variation (% CV) below 15% in all tested cases. The study showed the usefulness of the proposed method for the isolation of antidepressant drugs and their metabolites in saliva patients’ samples.
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spelling pubmed-100458042023-03-29 Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE) Dziurkowska, Ewelina Wesolowski, Marek Biomedicines Article The determination of antidepressant drugs and their metabolites in the body, mainly in the blood, allows for the monitoring of drug levels and their metabolism, helps identify drug interactions, and reduces the likelihood of increased side effects. Due to numerous inconveniences associated with collecting blood in patients, therapeutic drug monitoring (TDM) based on saliva sampling could significantly improve patient comfort. Therefore, the aim of this study was to develop a method for the simultaneous determination of selected antidepressants (amitriptyline, mianserin, duloxetine, mirtazapine, sertraline, citalopram, and venlafaxine) and their metabolites (N-desmethylmirtazapine, norsertraline, N-desmethylcitalopram, O-desmethylvenlafaxine) in human saliva using supported liquid extraction (SLE). Chlordiazepoxide was used as an internal standard. UHPLC coupled with DAD detection was used for the determinations. The proposed method was validated by determining its linearity for saliva concentrations in the range 10–1000 ng/mL. For all the analyzed compounds, a linear relationship between the analytical signal and analyte concentration was obtained (R(2) > 0.99), with the intra- and inter-day precisions expressed as a coefficient of variation (% CV) below 15% in all tested cases. The study showed the usefulness of the proposed method for the isolation of antidepressant drugs and their metabolites in saliva patients’ samples. MDPI 2023-02-25 /pmc/articles/PMC10045804/ /pubmed/36979687 http://dx.doi.org/10.3390/biomedicines11030708 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dziurkowska, Ewelina
Wesolowski, Marek
Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE)
title Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE)
title_full Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE)
title_fullStr Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE)
title_full_unstemmed Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE)
title_short Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE)
title_sort isolation of antidepressants and their metabolites from saliva using supported liquid extraction (sle)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045804/
https://www.ncbi.nlm.nih.gov/pubmed/36979687
http://dx.doi.org/10.3390/biomedicines11030708
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