Cargando…
Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE)
The determination of antidepressant drugs and their metabolites in the body, mainly in the blood, allows for the monitoring of drug levels and their metabolism, helps identify drug interactions, and reduces the likelihood of increased side effects. Due to numerous inconveniences associated with coll...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045804/ https://www.ncbi.nlm.nih.gov/pubmed/36979687 http://dx.doi.org/10.3390/biomedicines11030708 |
_version_ | 1785013508727898112 |
---|---|
author | Dziurkowska, Ewelina Wesolowski, Marek |
author_facet | Dziurkowska, Ewelina Wesolowski, Marek |
author_sort | Dziurkowska, Ewelina |
collection | PubMed |
description | The determination of antidepressant drugs and their metabolites in the body, mainly in the blood, allows for the monitoring of drug levels and their metabolism, helps identify drug interactions, and reduces the likelihood of increased side effects. Due to numerous inconveniences associated with collecting blood in patients, therapeutic drug monitoring (TDM) based on saliva sampling could significantly improve patient comfort. Therefore, the aim of this study was to develop a method for the simultaneous determination of selected antidepressants (amitriptyline, mianserin, duloxetine, mirtazapine, sertraline, citalopram, and venlafaxine) and their metabolites (N-desmethylmirtazapine, norsertraline, N-desmethylcitalopram, O-desmethylvenlafaxine) in human saliva using supported liquid extraction (SLE). Chlordiazepoxide was used as an internal standard. UHPLC coupled with DAD detection was used for the determinations. The proposed method was validated by determining its linearity for saliva concentrations in the range 10–1000 ng/mL. For all the analyzed compounds, a linear relationship between the analytical signal and analyte concentration was obtained (R(2) > 0.99), with the intra- and inter-day precisions expressed as a coefficient of variation (% CV) below 15% in all tested cases. The study showed the usefulness of the proposed method for the isolation of antidepressant drugs and their metabolites in saliva patients’ samples. |
format | Online Article Text |
id | pubmed-10045804 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100458042023-03-29 Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE) Dziurkowska, Ewelina Wesolowski, Marek Biomedicines Article The determination of antidepressant drugs and their metabolites in the body, mainly in the blood, allows for the monitoring of drug levels and their metabolism, helps identify drug interactions, and reduces the likelihood of increased side effects. Due to numerous inconveniences associated with collecting blood in patients, therapeutic drug monitoring (TDM) based on saliva sampling could significantly improve patient comfort. Therefore, the aim of this study was to develop a method for the simultaneous determination of selected antidepressants (amitriptyline, mianserin, duloxetine, mirtazapine, sertraline, citalopram, and venlafaxine) and their metabolites (N-desmethylmirtazapine, norsertraline, N-desmethylcitalopram, O-desmethylvenlafaxine) in human saliva using supported liquid extraction (SLE). Chlordiazepoxide was used as an internal standard. UHPLC coupled with DAD detection was used for the determinations. The proposed method was validated by determining its linearity for saliva concentrations in the range 10–1000 ng/mL. For all the analyzed compounds, a linear relationship between the analytical signal and analyte concentration was obtained (R(2) > 0.99), with the intra- and inter-day precisions expressed as a coefficient of variation (% CV) below 15% in all tested cases. The study showed the usefulness of the proposed method for the isolation of antidepressant drugs and their metabolites in saliva patients’ samples. MDPI 2023-02-25 /pmc/articles/PMC10045804/ /pubmed/36979687 http://dx.doi.org/10.3390/biomedicines11030708 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dziurkowska, Ewelina Wesolowski, Marek Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE) |
title | Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE) |
title_full | Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE) |
title_fullStr | Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE) |
title_full_unstemmed | Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE) |
title_short | Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE) |
title_sort | isolation of antidepressants and their metabolites from saliva using supported liquid extraction (sle) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045804/ https://www.ncbi.nlm.nih.gov/pubmed/36979687 http://dx.doi.org/10.3390/biomedicines11030708 |
work_keys_str_mv | AT dziurkowskaewelina isolationofantidepressantsandtheirmetabolitesfromsalivausingsupportedliquidextractionsle AT wesolowskimarek isolationofantidepressantsandtheirmetabolitesfromsalivausingsupportedliquidextractionsle |