Cargando…

Antioxidant Defense Expressed as Glutathione Status and Keap1-Nrf2 System Action in Relation to Anthropometric Parameters and Body Composition in Young Women with Polycystic Ovary Syndrome

Metabolic disorders present in women with polycystic ovary syndrome (PCOS) and the associated risk of obesity may result in increased oxidative stress and reproductive failure. Therefore, we evaluated the concentrations of reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione peroxidas...

Descripción completa

Detalles Bibliográficos
Autores principales: Chełchowska, Magdalena, Jurczewska, Justyna, Gajewska, Joanna, Mazur, Joanna, Szostak-Węgierek, Dorota, Rudnicka, Ewa, Ambroszkiewicz, Jadwiga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045817/
https://www.ncbi.nlm.nih.gov/pubmed/36978978
http://dx.doi.org/10.3390/antiox12030730
_version_ 1785013512236433408
author Chełchowska, Magdalena
Jurczewska, Justyna
Gajewska, Joanna
Mazur, Joanna
Szostak-Węgierek, Dorota
Rudnicka, Ewa
Ambroszkiewicz, Jadwiga
author_facet Chełchowska, Magdalena
Jurczewska, Justyna
Gajewska, Joanna
Mazur, Joanna
Szostak-Węgierek, Dorota
Rudnicka, Ewa
Ambroszkiewicz, Jadwiga
author_sort Chełchowska, Magdalena
collection PubMed
description Metabolic disorders present in women with polycystic ovary syndrome (PCOS) and the associated risk of obesity may result in increased oxidative stress and reproductive failure. Therefore, we evaluated the concentrations of reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione peroxidase (GPx), and reductase (GR), as well as nuclear factor erythroid 2-related factor 2 (Nrf2) and Kelch-like ECH-associating protein1 (Keap1) in the serum of 56 women with PCOS divided according to the visceral to subcutaneous fat surface ratio (VAT/SAT) and waist-to-hip ratio (WHR) values. Antioxidant parameter levels were measured by competitive inhibition enzyme immunoassay technique. As the VAT/SAT ratio and WHR increased, we observed significantly higher concentrations of GSSG and Keap1 protein and a lower value of the GSSG/GSH ratio (R-index), which is considered an index of cellular redox (p < 0.05). Negative correlations were found between the R-index and body weight, BMI, WHR, subcutaneous and visceral fat surface and the VAT/SAT ratio, and total body fat; positive links were found with fat free mass and total body water. Opposite associations were noted between GSSG level and the aforementioned body composition parameters. Oxidative stress characterized by a depleted reduced-to-oxidized glutathione index is associated with anthropometric and body composition parameters in women with PCOS. In particular, abdominal obesity expressed by the VAT/SAT ratio and/or WHR seems to have a negative impact on glutathione status, which may lead to a disruption of many biological cell processes. The observed negative association of Keap1 with R-index suggests that the elevated oxidative changes dependent on the VAT/SAT ratio may lead to Nrf2 activation to promote antioxidant enzyme expression. Although the GSH/GSSG index as well as the VAT/SAT ratio appear to be good indicators of oxidative status, studies on a larger group of patients should continue to confirm these links among women with PCOS.
format Online
Article
Text
id pubmed-10045817
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100458172023-03-29 Antioxidant Defense Expressed as Glutathione Status and Keap1-Nrf2 System Action in Relation to Anthropometric Parameters and Body Composition in Young Women with Polycystic Ovary Syndrome Chełchowska, Magdalena Jurczewska, Justyna Gajewska, Joanna Mazur, Joanna Szostak-Węgierek, Dorota Rudnicka, Ewa Ambroszkiewicz, Jadwiga Antioxidants (Basel) Article Metabolic disorders present in women with polycystic ovary syndrome (PCOS) and the associated risk of obesity may result in increased oxidative stress and reproductive failure. Therefore, we evaluated the concentrations of reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione peroxidase (GPx), and reductase (GR), as well as nuclear factor erythroid 2-related factor 2 (Nrf2) and Kelch-like ECH-associating protein1 (Keap1) in the serum of 56 women with PCOS divided according to the visceral to subcutaneous fat surface ratio (VAT/SAT) and waist-to-hip ratio (WHR) values. Antioxidant parameter levels were measured by competitive inhibition enzyme immunoassay technique. As the VAT/SAT ratio and WHR increased, we observed significantly higher concentrations of GSSG and Keap1 protein and a lower value of the GSSG/GSH ratio (R-index), which is considered an index of cellular redox (p < 0.05). Negative correlations were found between the R-index and body weight, BMI, WHR, subcutaneous and visceral fat surface and the VAT/SAT ratio, and total body fat; positive links were found with fat free mass and total body water. Opposite associations were noted between GSSG level and the aforementioned body composition parameters. Oxidative stress characterized by a depleted reduced-to-oxidized glutathione index is associated with anthropometric and body composition parameters in women with PCOS. In particular, abdominal obesity expressed by the VAT/SAT ratio and/or WHR seems to have a negative impact on glutathione status, which may lead to a disruption of many biological cell processes. The observed negative association of Keap1 with R-index suggests that the elevated oxidative changes dependent on the VAT/SAT ratio may lead to Nrf2 activation to promote antioxidant enzyme expression. Although the GSH/GSSG index as well as the VAT/SAT ratio appear to be good indicators of oxidative status, studies on a larger group of patients should continue to confirm these links among women with PCOS. MDPI 2023-03-16 /pmc/articles/PMC10045817/ /pubmed/36978978 http://dx.doi.org/10.3390/antiox12030730 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chełchowska, Magdalena
Jurczewska, Justyna
Gajewska, Joanna
Mazur, Joanna
Szostak-Węgierek, Dorota
Rudnicka, Ewa
Ambroszkiewicz, Jadwiga
Antioxidant Defense Expressed as Glutathione Status and Keap1-Nrf2 System Action in Relation to Anthropometric Parameters and Body Composition in Young Women with Polycystic Ovary Syndrome
title Antioxidant Defense Expressed as Glutathione Status and Keap1-Nrf2 System Action in Relation to Anthropometric Parameters and Body Composition in Young Women with Polycystic Ovary Syndrome
title_full Antioxidant Defense Expressed as Glutathione Status and Keap1-Nrf2 System Action in Relation to Anthropometric Parameters and Body Composition in Young Women with Polycystic Ovary Syndrome
title_fullStr Antioxidant Defense Expressed as Glutathione Status and Keap1-Nrf2 System Action in Relation to Anthropometric Parameters and Body Composition in Young Women with Polycystic Ovary Syndrome
title_full_unstemmed Antioxidant Defense Expressed as Glutathione Status and Keap1-Nrf2 System Action in Relation to Anthropometric Parameters and Body Composition in Young Women with Polycystic Ovary Syndrome
title_short Antioxidant Defense Expressed as Glutathione Status and Keap1-Nrf2 System Action in Relation to Anthropometric Parameters and Body Composition in Young Women with Polycystic Ovary Syndrome
title_sort antioxidant defense expressed as glutathione status and keap1-nrf2 system action in relation to anthropometric parameters and body composition in young women with polycystic ovary syndrome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045817/
https://www.ncbi.nlm.nih.gov/pubmed/36978978
http://dx.doi.org/10.3390/antiox12030730
work_keys_str_mv AT chełchowskamagdalena antioxidantdefenseexpressedasglutathionestatusandkeap1nrf2systemactioninrelationtoanthropometricparametersandbodycompositioninyoungwomenwithpolycysticovarysyndrome
AT jurczewskajustyna antioxidantdefenseexpressedasglutathionestatusandkeap1nrf2systemactioninrelationtoanthropometricparametersandbodycompositioninyoungwomenwithpolycysticovarysyndrome
AT gajewskajoanna antioxidantdefenseexpressedasglutathionestatusandkeap1nrf2systemactioninrelationtoanthropometricparametersandbodycompositioninyoungwomenwithpolycysticovarysyndrome
AT mazurjoanna antioxidantdefenseexpressedasglutathionestatusandkeap1nrf2systemactioninrelationtoanthropometricparametersandbodycompositioninyoungwomenwithpolycysticovarysyndrome
AT szostakwegierekdorota antioxidantdefenseexpressedasglutathionestatusandkeap1nrf2systemactioninrelationtoanthropometricparametersandbodycompositioninyoungwomenwithpolycysticovarysyndrome
AT rudnickaewa antioxidantdefenseexpressedasglutathionestatusandkeap1nrf2systemactioninrelationtoanthropometricparametersandbodycompositioninyoungwomenwithpolycysticovarysyndrome
AT ambroszkiewiczjadwiga antioxidantdefenseexpressedasglutathionestatusandkeap1nrf2systemactioninrelationtoanthropometricparametersandbodycompositioninyoungwomenwithpolycysticovarysyndrome