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Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study
Background and aims: A highly sensitive and specific point-of-care method for diagnosing spontaneous bacterial peritonitis (SBP) is currently lacking. The objective of the present study is to evaluate the diagnostic value of a rapid, easy-to-use, mid-infrared fiber evanescent wave spectroscopy (MIR-...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045833/ https://www.ncbi.nlm.nih.gov/pubmed/36979817 http://dx.doi.org/10.3390/biomedicines11030838 |
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author | Farrugia, Marwin A. Le Corvec, Maëna Renou, Christophe Nousbaum, Jean-Baptiste Ouizeman, Dann J. Sire, Olivier Loréal, Olivier Tariel, Hugues Bernard, Jérôme Piche, Thierry Tran, Albert Ait-Oufella, Hafid Landraud, Luce Gual, Philippe Anty, Rodolphe |
author_facet | Farrugia, Marwin A. Le Corvec, Maëna Renou, Christophe Nousbaum, Jean-Baptiste Ouizeman, Dann J. Sire, Olivier Loréal, Olivier Tariel, Hugues Bernard, Jérôme Piche, Thierry Tran, Albert Ait-Oufella, Hafid Landraud, Luce Gual, Philippe Anty, Rodolphe |
author_sort | Farrugia, Marwin A. |
collection | PubMed |
description | Background and aims: A highly sensitive and specific point-of-care method for diagnosing spontaneous bacterial peritonitis (SBP) is currently lacking. The objective of the present study is to evaluate the diagnostic value of a rapid, easy-to-use, mid-infrared fiber evanescent wave spectroscopy (MIR-FEWS) method for ruling out SBP. Patients and Methods: Cirrhotic patients (n = 256) at five centers in France were included for suspected SBP or for the scheduled evacuation of ascites fluid. The mid-infrared spectrum of 7 µL of an ascites fluid sample was recorded using a MIR-FEWS system. To define a model for the diagnosis of SBP, the patients were divided into a calibration group (n = 170) and a validation group (n = 86). Results: Most of the patients were male (71%). The mean age was 60.25 years. Alcohol-related liver disease was the most common cause of cirrhosis. SBP was observed in 18% of the patients. For the diagnosis of SBP in the calibration and validation groups, respectively, the model gave areas under the receiver operating characteristic curves of 0.87 and 0.89, sensitivities of 90% and 87%, specificities of 78% and 80%, positive predictive values of 48% and 50%, negative predictive values of 97% and 96%, positive likelihood ratio of 4.09 and 4.35, negative likelihood ratio of 0.13 and 0.16, Youden index of 0.68 and 0.67, and correct classification rates of 80% and 81%. Conclusion: The results of this proof-of-concept study show that MIR-FEWS is a highly sensitive diagnostic method for ruling out SBP. The method warrants further investigation. |
format | Online Article Text |
id | pubmed-10045833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100458332023-03-29 Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study Farrugia, Marwin A. Le Corvec, Maëna Renou, Christophe Nousbaum, Jean-Baptiste Ouizeman, Dann J. Sire, Olivier Loréal, Olivier Tariel, Hugues Bernard, Jérôme Piche, Thierry Tran, Albert Ait-Oufella, Hafid Landraud, Luce Gual, Philippe Anty, Rodolphe Biomedicines Article Background and aims: A highly sensitive and specific point-of-care method for diagnosing spontaneous bacterial peritonitis (SBP) is currently lacking. The objective of the present study is to evaluate the diagnostic value of a rapid, easy-to-use, mid-infrared fiber evanescent wave spectroscopy (MIR-FEWS) method for ruling out SBP. Patients and Methods: Cirrhotic patients (n = 256) at five centers in France were included for suspected SBP or for the scheduled evacuation of ascites fluid. The mid-infrared spectrum of 7 µL of an ascites fluid sample was recorded using a MIR-FEWS system. To define a model for the diagnosis of SBP, the patients were divided into a calibration group (n = 170) and a validation group (n = 86). Results: Most of the patients were male (71%). The mean age was 60.25 years. Alcohol-related liver disease was the most common cause of cirrhosis. SBP was observed in 18% of the patients. For the diagnosis of SBP in the calibration and validation groups, respectively, the model gave areas under the receiver operating characteristic curves of 0.87 and 0.89, sensitivities of 90% and 87%, specificities of 78% and 80%, positive predictive values of 48% and 50%, negative predictive values of 97% and 96%, positive likelihood ratio of 4.09 and 4.35, negative likelihood ratio of 0.13 and 0.16, Youden index of 0.68 and 0.67, and correct classification rates of 80% and 81%. Conclusion: The results of this proof-of-concept study show that MIR-FEWS is a highly sensitive diagnostic method for ruling out SBP. The method warrants further investigation. MDPI 2023-03-09 /pmc/articles/PMC10045833/ /pubmed/36979817 http://dx.doi.org/10.3390/biomedicines11030838 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Farrugia, Marwin A. Le Corvec, Maëna Renou, Christophe Nousbaum, Jean-Baptiste Ouizeman, Dann J. Sire, Olivier Loréal, Olivier Tariel, Hugues Bernard, Jérôme Piche, Thierry Tran, Albert Ait-Oufella, Hafid Landraud, Luce Gual, Philippe Anty, Rodolphe Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study |
title | Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study |
title_full | Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study |
title_fullStr | Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study |
title_full_unstemmed | Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study |
title_short | Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study |
title_sort | mid-infrared spectroscopy as a new tool for ruling out spontaneous bacterial peritonitis: a proof-of-concept study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045833/ https://www.ncbi.nlm.nih.gov/pubmed/36979817 http://dx.doi.org/10.3390/biomedicines11030838 |
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