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Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study

Background and aims: A highly sensitive and specific point-of-care method for diagnosing spontaneous bacterial peritonitis (SBP) is currently lacking. The objective of the present study is to evaluate the diagnostic value of a rapid, easy-to-use, mid-infrared fiber evanescent wave spectroscopy (MIR-...

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Autores principales: Farrugia, Marwin A., Le Corvec, Maëna, Renou, Christophe, Nousbaum, Jean-Baptiste, Ouizeman, Dann J., Sire, Olivier, Loréal, Olivier, Tariel, Hugues, Bernard, Jérôme, Piche, Thierry, Tran, Albert, Ait-Oufella, Hafid, Landraud, Luce, Gual, Philippe, Anty, Rodolphe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045833/
https://www.ncbi.nlm.nih.gov/pubmed/36979817
http://dx.doi.org/10.3390/biomedicines11030838
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author Farrugia, Marwin A.
Le Corvec, Maëna
Renou, Christophe
Nousbaum, Jean-Baptiste
Ouizeman, Dann J.
Sire, Olivier
Loréal, Olivier
Tariel, Hugues
Bernard, Jérôme
Piche, Thierry
Tran, Albert
Ait-Oufella, Hafid
Landraud, Luce
Gual, Philippe
Anty, Rodolphe
author_facet Farrugia, Marwin A.
Le Corvec, Maëna
Renou, Christophe
Nousbaum, Jean-Baptiste
Ouizeman, Dann J.
Sire, Olivier
Loréal, Olivier
Tariel, Hugues
Bernard, Jérôme
Piche, Thierry
Tran, Albert
Ait-Oufella, Hafid
Landraud, Luce
Gual, Philippe
Anty, Rodolphe
author_sort Farrugia, Marwin A.
collection PubMed
description Background and aims: A highly sensitive and specific point-of-care method for diagnosing spontaneous bacterial peritonitis (SBP) is currently lacking. The objective of the present study is to evaluate the diagnostic value of a rapid, easy-to-use, mid-infrared fiber evanescent wave spectroscopy (MIR-FEWS) method for ruling out SBP. Patients and Methods: Cirrhotic patients (n = 256) at five centers in France were included for suspected SBP or for the scheduled evacuation of ascites fluid. The mid-infrared spectrum of 7 µL of an ascites fluid sample was recorded using a MIR-FEWS system. To define a model for the diagnosis of SBP, the patients were divided into a calibration group (n = 170) and a validation group (n = 86). Results: Most of the patients were male (71%). The mean age was 60.25 years. Alcohol-related liver disease was the most common cause of cirrhosis. SBP was observed in 18% of the patients. For the diagnosis of SBP in the calibration and validation groups, respectively, the model gave areas under the receiver operating characteristic curves of 0.87 and 0.89, sensitivities of 90% and 87%, specificities of 78% and 80%, positive predictive values of 48% and 50%, negative predictive values of 97% and 96%, positive likelihood ratio of 4.09 and 4.35, negative likelihood ratio of 0.13 and 0.16, Youden index of 0.68 and 0.67, and correct classification rates of 80% and 81%. Conclusion: The results of this proof-of-concept study show that MIR-FEWS is a highly sensitive diagnostic method for ruling out SBP. The method warrants further investigation.
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spelling pubmed-100458332023-03-29 Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study Farrugia, Marwin A. Le Corvec, Maëna Renou, Christophe Nousbaum, Jean-Baptiste Ouizeman, Dann J. Sire, Olivier Loréal, Olivier Tariel, Hugues Bernard, Jérôme Piche, Thierry Tran, Albert Ait-Oufella, Hafid Landraud, Luce Gual, Philippe Anty, Rodolphe Biomedicines Article Background and aims: A highly sensitive and specific point-of-care method for diagnosing spontaneous bacterial peritonitis (SBP) is currently lacking. The objective of the present study is to evaluate the diagnostic value of a rapid, easy-to-use, mid-infrared fiber evanescent wave spectroscopy (MIR-FEWS) method for ruling out SBP. Patients and Methods: Cirrhotic patients (n = 256) at five centers in France were included for suspected SBP or for the scheduled evacuation of ascites fluid. The mid-infrared spectrum of 7 µL of an ascites fluid sample was recorded using a MIR-FEWS system. To define a model for the diagnosis of SBP, the patients were divided into a calibration group (n = 170) and a validation group (n = 86). Results: Most of the patients were male (71%). The mean age was 60.25 years. Alcohol-related liver disease was the most common cause of cirrhosis. SBP was observed in 18% of the patients. For the diagnosis of SBP in the calibration and validation groups, respectively, the model gave areas under the receiver operating characteristic curves of 0.87 and 0.89, sensitivities of 90% and 87%, specificities of 78% and 80%, positive predictive values of 48% and 50%, negative predictive values of 97% and 96%, positive likelihood ratio of 4.09 and 4.35, negative likelihood ratio of 0.13 and 0.16, Youden index of 0.68 and 0.67, and correct classification rates of 80% and 81%. Conclusion: The results of this proof-of-concept study show that MIR-FEWS is a highly sensitive diagnostic method for ruling out SBP. The method warrants further investigation. MDPI 2023-03-09 /pmc/articles/PMC10045833/ /pubmed/36979817 http://dx.doi.org/10.3390/biomedicines11030838 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Farrugia, Marwin A.
Le Corvec, Maëna
Renou, Christophe
Nousbaum, Jean-Baptiste
Ouizeman, Dann J.
Sire, Olivier
Loréal, Olivier
Tariel, Hugues
Bernard, Jérôme
Piche, Thierry
Tran, Albert
Ait-Oufella, Hafid
Landraud, Luce
Gual, Philippe
Anty, Rodolphe
Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study
title Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study
title_full Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study
title_fullStr Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study
title_full_unstemmed Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study
title_short Mid-Infrared Spectroscopy as a New Tool for Ruling Out Spontaneous Bacterial Peritonitis: A Proof-of-Concept Study
title_sort mid-infrared spectroscopy as a new tool for ruling out spontaneous bacterial peritonitis: a proof-of-concept study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045833/
https://www.ncbi.nlm.nih.gov/pubmed/36979817
http://dx.doi.org/10.3390/biomedicines11030838
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