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Analysis of the Influence of IL-6 and the Activation of the Jak/Stat3 Pathway in Fibromyalgia
Background: Fibromyalgia is a medical condition that affects a small percentage of the population, with no known effective treatment. There is evidence to suggest that inflammation is a key factor in the nerve sensitization that characterizes the disorder. Therefore, this paper concentrates on the r...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045851/ https://www.ncbi.nlm.nih.gov/pubmed/36979771 http://dx.doi.org/10.3390/biomedicines11030792 |
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author | Marino, Ylenia Arangia, Alessia Cordaro, Marika Siracusa, Rosalba D’Amico, Ramona Impellizzeri, Daniela Cupi, Rosalia Peritore, Alessio Filippo Gugliandolo, Enrico Fusco, Roberta Cuzzocrea, Salvatore Di Paola, Rosanna |
author_facet | Marino, Ylenia Arangia, Alessia Cordaro, Marika Siracusa, Rosalba D’Amico, Ramona Impellizzeri, Daniela Cupi, Rosalia Peritore, Alessio Filippo Gugliandolo, Enrico Fusco, Roberta Cuzzocrea, Salvatore Di Paola, Rosanna |
author_sort | Marino, Ylenia |
collection | PubMed |
description | Background: Fibromyalgia is a medical condition that affects a small percentage of the population, with no known effective treatment. There is evidence to suggest that inflammation is a key factor in the nerve sensitization that characterizes the disorder. Therefore, this paper concentrates on the role of IL-6 in fibromyalgia and the related pain-like symptoms. Methods: This work aimed to evaluate Sprague–Dawley rats, which were injected for three consecutive days with 1 mg/kg of reserpine; IL-6-R Ab was intraperitoneally injected at 1.5 mg/kg seven days after the first reserpine injection. Behavioral analyses were conducted at the beginning of the experiment and at seven and twenty-one days from the first reserpine injection. At this timepoint, the animals were sacrificed, and tissues were collected for molecular and histological analysis. Results: Our data showed the analgesic effect of IL-6-R-Ab administration on mechanical allodynia and thermal hyperalgesia. Additionally, the reserpine + IL-6-R-Ab group showed a reduced expression of the pain-related mediators cFOS and NFG and reduced levels of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) and chemokines (Cxcl5, Cxcl10 and Cx3cl1). From the molecular point of view, the IL-6-R-Ab administration reduced the gp130 phosphorylation and the activation of the Jak/STAT3 pathway. Additionally, the IL-6-R Ab reduced the activation of neuroinflammatory cells. Conclusions: Our study showed that IL-6 plays a crucial role in fibromyalgia by triggering the Jak/STAT3 pathway, leading to an increase in chemokine levels and activating glial cells. |
format | Online Article Text |
id | pubmed-10045851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100458512023-03-29 Analysis of the Influence of IL-6 and the Activation of the Jak/Stat3 Pathway in Fibromyalgia Marino, Ylenia Arangia, Alessia Cordaro, Marika Siracusa, Rosalba D’Amico, Ramona Impellizzeri, Daniela Cupi, Rosalia Peritore, Alessio Filippo Gugliandolo, Enrico Fusco, Roberta Cuzzocrea, Salvatore Di Paola, Rosanna Biomedicines Article Background: Fibromyalgia is a medical condition that affects a small percentage of the population, with no known effective treatment. There is evidence to suggest that inflammation is a key factor in the nerve sensitization that characterizes the disorder. Therefore, this paper concentrates on the role of IL-6 in fibromyalgia and the related pain-like symptoms. Methods: This work aimed to evaluate Sprague–Dawley rats, which were injected for three consecutive days with 1 mg/kg of reserpine; IL-6-R Ab was intraperitoneally injected at 1.5 mg/kg seven days after the first reserpine injection. Behavioral analyses were conducted at the beginning of the experiment and at seven and twenty-one days from the first reserpine injection. At this timepoint, the animals were sacrificed, and tissues were collected for molecular and histological analysis. Results: Our data showed the analgesic effect of IL-6-R-Ab administration on mechanical allodynia and thermal hyperalgesia. Additionally, the reserpine + IL-6-R-Ab group showed a reduced expression of the pain-related mediators cFOS and NFG and reduced levels of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) and chemokines (Cxcl5, Cxcl10 and Cx3cl1). From the molecular point of view, the IL-6-R-Ab administration reduced the gp130 phosphorylation and the activation of the Jak/STAT3 pathway. Additionally, the IL-6-R Ab reduced the activation of neuroinflammatory cells. Conclusions: Our study showed that IL-6 plays a crucial role in fibromyalgia by triggering the Jak/STAT3 pathway, leading to an increase in chemokine levels and activating glial cells. MDPI 2023-03-06 /pmc/articles/PMC10045851/ /pubmed/36979771 http://dx.doi.org/10.3390/biomedicines11030792 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Marino, Ylenia Arangia, Alessia Cordaro, Marika Siracusa, Rosalba D’Amico, Ramona Impellizzeri, Daniela Cupi, Rosalia Peritore, Alessio Filippo Gugliandolo, Enrico Fusco, Roberta Cuzzocrea, Salvatore Di Paola, Rosanna Analysis of the Influence of IL-6 and the Activation of the Jak/Stat3 Pathway in Fibromyalgia |
title | Analysis of the Influence of IL-6 and the Activation of the Jak/Stat3 Pathway in Fibromyalgia |
title_full | Analysis of the Influence of IL-6 and the Activation of the Jak/Stat3 Pathway in Fibromyalgia |
title_fullStr | Analysis of the Influence of IL-6 and the Activation of the Jak/Stat3 Pathway in Fibromyalgia |
title_full_unstemmed | Analysis of the Influence of IL-6 and the Activation of the Jak/Stat3 Pathway in Fibromyalgia |
title_short | Analysis of the Influence of IL-6 and the Activation of the Jak/Stat3 Pathway in Fibromyalgia |
title_sort | analysis of the influence of il-6 and the activation of the jak/stat3 pathway in fibromyalgia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045851/ https://www.ncbi.nlm.nih.gov/pubmed/36979771 http://dx.doi.org/10.3390/biomedicines11030792 |
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