Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-19 Pandemic

SIMPLE SUMMARY: Acinetobacter baumannii is a problematic bacterium that causes hard-to-treat hospital infections worldwide. Multiple cases of A. baumannii/SARS-CoV-2 co-infection were reported during the pandemic. This fact raised the question of whether the strains in those co-infections had or acquired unique genetic traits. This study is a comparative analysis of two strains from the same clonal group, but one was isolated before the pandemic, and the other was isolated from a patient with COVID-19. Their genomes had a high similarity, indicating that they may have derived from a unique background. However, each genome had numerous unique genes that were involved in virulence and resistance to antimicrobials. These differences could result from adaptative evolution to the human body infected with SARS-CoV-2. ABSTRACT: Background: After the emergence of COVID-19, numerous cases of A. baumannii/SARS-CoV-2 co-infection were reported. Whether the co-infecting A. baumannii strains have distinctive characteristics remains unknown. Methods and Results: A. baumannii AMA_NO was isolated in 2021 from a patient with COVID-19. AMA166 was isolated from a mini-BAL used on a patient with pneumonia in 2016. Both genomes were similar, but they possessed 337 (AMA_NO) and 93 (AMA166) unique genes that were associated with biofilm formation, flagellar assembly, antibiotic resistance, secretion systems, and other functions. The antibiotic resistance genes were found within mobile genetic elements. While both strains harbored the carbapenemase-coding gene bla(OXA-23), only the strain AMA_NO carried bla(NDM-1). Representative functions coded for by virulence genes are the synthesis of the outer core of lipooligosaccharide (OCL5), biosynthesis and export of the capsular polysaccharide (KL2 cluster), high-efficiency iron uptake systems (acinetobactin and baumannoferrin), adherence, and quorum sensing. A comparative phylogenetic analysis including 239 additional sequence type (ST) 2 representative genomes showed high similarity to A. baumannii ABBL141. Since the degree of similarity that was observed between A. baumannii AMA_NO and AMA166 is higher than that found among other ST2 strains, we propose that they derive from a unique background based on core-genome phylogeny and comparative genome analysis. Conclusions: Acquisition or shedding of specific genes could increase the ability of A. baumannii to infect patients with COVID-19.