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An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells?
The evaluation of chronic pain is challenging because of the lack of specific biomarkers. We identified the Mu opioid receptor-positive (Mu+) B cell percentage of expression, named Mu-Lympho-Marker (MLM), as a candidate marker for chronic pain in fibromyalgia (FM) and osteoarthritis (OA) patients. H...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10046119/ https://www.ncbi.nlm.nih.gov/pubmed/36979910 http://dx.doi.org/10.3390/biomedicines11030931 |
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author | Malafoglia, Valentina Ilari, Sara Gioia, Chiara Vitiello, Laura Tenti, Michael Iannuccelli, Cristina Cristiani, Costanza Maria Garofalo, Cinzia Passacatini, Lucia Carmela Viglietto, Giuseppe Scavalli, Antonio Sili Tomino, Carlo Mollace, Vincenzo Raffaeli, William Di Franco, Manuela Muscoli, Carolina |
author_facet | Malafoglia, Valentina Ilari, Sara Gioia, Chiara Vitiello, Laura Tenti, Michael Iannuccelli, Cristina Cristiani, Costanza Maria Garofalo, Cinzia Passacatini, Lucia Carmela Viglietto, Giuseppe Scavalli, Antonio Sili Tomino, Carlo Mollace, Vincenzo Raffaeli, William Di Franco, Manuela Muscoli, Carolina |
author_sort | Malafoglia, Valentina |
collection | PubMed |
description | The evaluation of chronic pain is challenging because of the lack of specific biomarkers. We identified the Mu opioid receptor-positive (Mu+) B cell percentage of expression, named Mu-Lympho-Marker (MLM), as a candidate marker for chronic pain in fibromyalgia (FM) and osteoarthritis (OA) patients. Here, we investigate the role of MLM on natural killer (NK) cells in the same patients. Twenty-nine FM and twelve OA patients were analyzed, and twenty-three pain-free subjects were considered as the control group. Blood samples were collected to perform immunophenotyping and Western blot analysis. Biological and clinical data were statistically analyzed. The final results showed that the percentage of NK cells expressing Mu was statistically lower in FM and OA patients than in pain-free subjects, as already demonstrated for B cells. A Western blot analysis was performed in order to detect NK cells’ functional status. Moreover, the correlation analysis of MLM expression with pharmacological therapy did not show any significant results. In conclusion, here, we confirm the role of MLM as a suitable marker for chronic pain and underline NK cells as a new possible immune cell type involved in the “Mu opioid receptor reserve theory”. |
format | Online Article Text |
id | pubmed-10046119 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100461192023-03-29 An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells? Malafoglia, Valentina Ilari, Sara Gioia, Chiara Vitiello, Laura Tenti, Michael Iannuccelli, Cristina Cristiani, Costanza Maria Garofalo, Cinzia Passacatini, Lucia Carmela Viglietto, Giuseppe Scavalli, Antonio Sili Tomino, Carlo Mollace, Vincenzo Raffaeli, William Di Franco, Manuela Muscoli, Carolina Biomedicines Article The evaluation of chronic pain is challenging because of the lack of specific biomarkers. We identified the Mu opioid receptor-positive (Mu+) B cell percentage of expression, named Mu-Lympho-Marker (MLM), as a candidate marker for chronic pain in fibromyalgia (FM) and osteoarthritis (OA) patients. Here, we investigate the role of MLM on natural killer (NK) cells in the same patients. Twenty-nine FM and twelve OA patients were analyzed, and twenty-three pain-free subjects were considered as the control group. Blood samples were collected to perform immunophenotyping and Western blot analysis. Biological and clinical data were statistically analyzed. The final results showed that the percentage of NK cells expressing Mu was statistically lower in FM and OA patients than in pain-free subjects, as already demonstrated for B cells. A Western blot analysis was performed in order to detect NK cells’ functional status. Moreover, the correlation analysis of MLM expression with pharmacological therapy did not show any significant results. In conclusion, here, we confirm the role of MLM as a suitable marker for chronic pain and underline NK cells as a new possible immune cell type involved in the “Mu opioid receptor reserve theory”. MDPI 2023-03-17 /pmc/articles/PMC10046119/ /pubmed/36979910 http://dx.doi.org/10.3390/biomedicines11030931 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Malafoglia, Valentina Ilari, Sara Gioia, Chiara Vitiello, Laura Tenti, Michael Iannuccelli, Cristina Cristiani, Costanza Maria Garofalo, Cinzia Passacatini, Lucia Carmela Viglietto, Giuseppe Scavalli, Antonio Sili Tomino, Carlo Mollace, Vincenzo Raffaeli, William Di Franco, Manuela Muscoli, Carolina An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells? |
title | An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells? |
title_full | An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells? |
title_fullStr | An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells? |
title_full_unstemmed | An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells? |
title_short | An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells? |
title_sort | observational study on chronic pain biomarkers in fibromyalgia and osteoarthritis patients: which role for mu opioid receptor’s expression on nk cells? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10046119/ https://www.ncbi.nlm.nih.gov/pubmed/36979910 http://dx.doi.org/10.3390/biomedicines11030931 |
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