Cargando…

An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells?

The evaluation of chronic pain is challenging because of the lack of specific biomarkers. We identified the Mu opioid receptor-positive (Mu+) B cell percentage of expression, named Mu-Lympho-Marker (MLM), as a candidate marker for chronic pain in fibromyalgia (FM) and osteoarthritis (OA) patients. H...

Descripción completa

Detalles Bibliográficos
Autores principales: Malafoglia, Valentina, Ilari, Sara, Gioia, Chiara, Vitiello, Laura, Tenti, Michael, Iannuccelli, Cristina, Cristiani, Costanza Maria, Garofalo, Cinzia, Passacatini, Lucia Carmela, Viglietto, Giuseppe, Scavalli, Antonio Sili, Tomino, Carlo, Mollace, Vincenzo, Raffaeli, William, Di Franco, Manuela, Muscoli, Carolina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10046119/
https://www.ncbi.nlm.nih.gov/pubmed/36979910
http://dx.doi.org/10.3390/biomedicines11030931
_version_ 1785013586229198848
author Malafoglia, Valentina
Ilari, Sara
Gioia, Chiara
Vitiello, Laura
Tenti, Michael
Iannuccelli, Cristina
Cristiani, Costanza Maria
Garofalo, Cinzia
Passacatini, Lucia Carmela
Viglietto, Giuseppe
Scavalli, Antonio Sili
Tomino, Carlo
Mollace, Vincenzo
Raffaeli, William
Di Franco, Manuela
Muscoli, Carolina
author_facet Malafoglia, Valentina
Ilari, Sara
Gioia, Chiara
Vitiello, Laura
Tenti, Michael
Iannuccelli, Cristina
Cristiani, Costanza Maria
Garofalo, Cinzia
Passacatini, Lucia Carmela
Viglietto, Giuseppe
Scavalli, Antonio Sili
Tomino, Carlo
Mollace, Vincenzo
Raffaeli, William
Di Franco, Manuela
Muscoli, Carolina
author_sort Malafoglia, Valentina
collection PubMed
description The evaluation of chronic pain is challenging because of the lack of specific biomarkers. We identified the Mu opioid receptor-positive (Mu+) B cell percentage of expression, named Mu-Lympho-Marker (MLM), as a candidate marker for chronic pain in fibromyalgia (FM) and osteoarthritis (OA) patients. Here, we investigate the role of MLM on natural killer (NK) cells in the same patients. Twenty-nine FM and twelve OA patients were analyzed, and twenty-three pain-free subjects were considered as the control group. Blood samples were collected to perform immunophenotyping and Western blot analysis. Biological and clinical data were statistically analyzed. The final results showed that the percentage of NK cells expressing Mu was statistically lower in FM and OA patients than in pain-free subjects, as already demonstrated for B cells. A Western blot analysis was performed in order to detect NK cells’ functional status. Moreover, the correlation analysis of MLM expression with pharmacological therapy did not show any significant results. In conclusion, here, we confirm the role of MLM as a suitable marker for chronic pain and underline NK cells as a new possible immune cell type involved in the “Mu opioid receptor reserve theory”.
format Online
Article
Text
id pubmed-10046119
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100461192023-03-29 An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells? Malafoglia, Valentina Ilari, Sara Gioia, Chiara Vitiello, Laura Tenti, Michael Iannuccelli, Cristina Cristiani, Costanza Maria Garofalo, Cinzia Passacatini, Lucia Carmela Viglietto, Giuseppe Scavalli, Antonio Sili Tomino, Carlo Mollace, Vincenzo Raffaeli, William Di Franco, Manuela Muscoli, Carolina Biomedicines Article The evaluation of chronic pain is challenging because of the lack of specific biomarkers. We identified the Mu opioid receptor-positive (Mu+) B cell percentage of expression, named Mu-Lympho-Marker (MLM), as a candidate marker for chronic pain in fibromyalgia (FM) and osteoarthritis (OA) patients. Here, we investigate the role of MLM on natural killer (NK) cells in the same patients. Twenty-nine FM and twelve OA patients were analyzed, and twenty-three pain-free subjects were considered as the control group. Blood samples were collected to perform immunophenotyping and Western blot analysis. Biological and clinical data were statistically analyzed. The final results showed that the percentage of NK cells expressing Mu was statistically lower in FM and OA patients than in pain-free subjects, as already demonstrated for B cells. A Western blot analysis was performed in order to detect NK cells’ functional status. Moreover, the correlation analysis of MLM expression with pharmacological therapy did not show any significant results. In conclusion, here, we confirm the role of MLM as a suitable marker for chronic pain and underline NK cells as a new possible immune cell type involved in the “Mu opioid receptor reserve theory”. MDPI 2023-03-17 /pmc/articles/PMC10046119/ /pubmed/36979910 http://dx.doi.org/10.3390/biomedicines11030931 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Malafoglia, Valentina
Ilari, Sara
Gioia, Chiara
Vitiello, Laura
Tenti, Michael
Iannuccelli, Cristina
Cristiani, Costanza Maria
Garofalo, Cinzia
Passacatini, Lucia Carmela
Viglietto, Giuseppe
Scavalli, Antonio Sili
Tomino, Carlo
Mollace, Vincenzo
Raffaeli, William
Di Franco, Manuela
Muscoli, Carolina
An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells?
title An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells?
title_full An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells?
title_fullStr An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells?
title_full_unstemmed An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells?
title_short An Observational Study on Chronic Pain Biomarkers in Fibromyalgia and Osteoarthritis Patients: Which Role for Mu Opioid Receptor’s Expression on NK Cells?
title_sort observational study on chronic pain biomarkers in fibromyalgia and osteoarthritis patients: which role for mu opioid receptor’s expression on nk cells?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10046119/
https://www.ncbi.nlm.nih.gov/pubmed/36979910
http://dx.doi.org/10.3390/biomedicines11030931
work_keys_str_mv AT malafogliavalentina anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT ilarisara anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT gioiachiara anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT vitiellolaura anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT tentimichael anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT iannuccellicristina anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT cristianicostanzamaria anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT garofalocinzia anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT passacatiniluciacarmela anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT vigliettogiuseppe anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT scavalliantoniosili anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT tominocarlo anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT mollacevincenzo anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT raffaeliwilliam anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT difrancomanuela anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT muscolicarolina anobservationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT malafogliavalentina observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT ilarisara observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT gioiachiara observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT vitiellolaura observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT tentimichael observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT iannuccellicristina observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT cristianicostanzamaria observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT garofalocinzia observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT passacatiniluciacarmela observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT vigliettogiuseppe observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT scavalliantoniosili observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT tominocarlo observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT mollacevincenzo observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT raffaeliwilliam observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT difrancomanuela observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells
AT muscolicarolina observationalstudyonchronicpainbiomarkersinfibromyalgiaandosteoarthritispatientswhichroleformuopioidreceptorsexpressiononnkcells