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Carrageenan from Gigartina skottsbergii: A Novel Molecular Probe to Detect SARS-CoV-2

The COVID-19 pandemic has caused an unprecedented health and economic crisis, highlighting the importance of developing new molecular tools to monitor and detect SARS-CoV-2. Hence, this study proposed to employ the carrageenan extracted from Gigartina skottsbergii algae as a probe for SARS-CoV-2 vir...

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Autores principales: Zank, Patrícia Daiane, Cerveira, Milena Mattes, dos Santos, Victor Barboza, Klein, Vitor Pereira, de Souza, Thobias Toniolo, Bueno, Danielle Tapia, Poletti, Tais, Leitzke, Amanda Fonseca, Luehring Giongo, Janice, Carreño, Neftali Lenin Villarreal, Mansilla, Andrés, Astorga-España, Maria Soledad, de Pereira, Claudio Martin Pereira, Vaucher, Rodrigo de Almeida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10046870/
https://www.ncbi.nlm.nih.gov/pubmed/36979590
http://dx.doi.org/10.3390/bios13030378
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author Zank, Patrícia Daiane
Cerveira, Milena Mattes
dos Santos, Victor Barboza
Klein, Vitor Pereira
de Souza, Thobias Toniolo
Bueno, Danielle Tapia
Poletti, Tais
Leitzke, Amanda Fonseca
Luehring Giongo, Janice
Carreño, Neftali Lenin Villarreal
Mansilla, Andrés
Astorga-España, Maria Soledad
de Pereira, Claudio Martin Pereira
Vaucher, Rodrigo de Almeida
author_facet Zank, Patrícia Daiane
Cerveira, Milena Mattes
dos Santos, Victor Barboza
Klein, Vitor Pereira
de Souza, Thobias Toniolo
Bueno, Danielle Tapia
Poletti, Tais
Leitzke, Amanda Fonseca
Luehring Giongo, Janice
Carreño, Neftali Lenin Villarreal
Mansilla, Andrés
Astorga-España, Maria Soledad
de Pereira, Claudio Martin Pereira
Vaucher, Rodrigo de Almeida
author_sort Zank, Patrícia Daiane
collection PubMed
description The COVID-19 pandemic has caused an unprecedented health and economic crisis, highlighting the importance of developing new molecular tools to monitor and detect SARS-CoV-2. Hence, this study proposed to employ the carrageenan extracted from Gigartina skottsbergii algae as a probe for SARS-CoV-2 virus binding capacity and potential use in molecular methods. G. skottsbergii specimens were collected in the Chilean subantarctic ecoregion, and the carrageenan was extracted —using a modified version of Webber’s method—, characterized, and quantified. After 24 h of incubation with an inactivated viral suspension, the carrageenan’s capacity to bind SARS-CoV-2 was tested. The probe-bound viral RNA was quantified using the reverse transcription and reverse transcription loop-mediated isothermal amplification (RT-LAMP) methods. Our findings showed that carrageenan extraction from seaweed has a similar spectrum to commercial carrageenan, achieving an excellent proportion of binding to SARS-CoV-2, with a yield of 8.3%. Viral RNA was also detected in the RT-LAMP assay. This study shows, for the first time, the binding capacity of carrageenan extracted from G. skottsbergii, which proved to be a low-cost and highly efficient method of binding to SARS-CoV-2 viral particles.
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spelling pubmed-100468702023-03-29 Carrageenan from Gigartina skottsbergii: A Novel Molecular Probe to Detect SARS-CoV-2 Zank, Patrícia Daiane Cerveira, Milena Mattes dos Santos, Victor Barboza Klein, Vitor Pereira de Souza, Thobias Toniolo Bueno, Danielle Tapia Poletti, Tais Leitzke, Amanda Fonseca Luehring Giongo, Janice Carreño, Neftali Lenin Villarreal Mansilla, Andrés Astorga-España, Maria Soledad de Pereira, Claudio Martin Pereira Vaucher, Rodrigo de Almeida Biosensors (Basel) Article The COVID-19 pandemic has caused an unprecedented health and economic crisis, highlighting the importance of developing new molecular tools to monitor and detect SARS-CoV-2. Hence, this study proposed to employ the carrageenan extracted from Gigartina skottsbergii algae as a probe for SARS-CoV-2 virus binding capacity and potential use in molecular methods. G. skottsbergii specimens were collected in the Chilean subantarctic ecoregion, and the carrageenan was extracted —using a modified version of Webber’s method—, characterized, and quantified. After 24 h of incubation with an inactivated viral suspension, the carrageenan’s capacity to bind SARS-CoV-2 was tested. The probe-bound viral RNA was quantified using the reverse transcription and reverse transcription loop-mediated isothermal amplification (RT-LAMP) methods. Our findings showed that carrageenan extraction from seaweed has a similar spectrum to commercial carrageenan, achieving an excellent proportion of binding to SARS-CoV-2, with a yield of 8.3%. Viral RNA was also detected in the RT-LAMP assay. This study shows, for the first time, the binding capacity of carrageenan extracted from G. skottsbergii, which proved to be a low-cost and highly efficient method of binding to SARS-CoV-2 viral particles. MDPI 2023-03-13 /pmc/articles/PMC10046870/ /pubmed/36979590 http://dx.doi.org/10.3390/bios13030378 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zank, Patrícia Daiane
Cerveira, Milena Mattes
dos Santos, Victor Barboza
Klein, Vitor Pereira
de Souza, Thobias Toniolo
Bueno, Danielle Tapia
Poletti, Tais
Leitzke, Amanda Fonseca
Luehring Giongo, Janice
Carreño, Neftali Lenin Villarreal
Mansilla, Andrés
Astorga-España, Maria Soledad
de Pereira, Claudio Martin Pereira
Vaucher, Rodrigo de Almeida
Carrageenan from Gigartina skottsbergii: A Novel Molecular Probe to Detect SARS-CoV-2
title Carrageenan from Gigartina skottsbergii: A Novel Molecular Probe to Detect SARS-CoV-2
title_full Carrageenan from Gigartina skottsbergii: A Novel Molecular Probe to Detect SARS-CoV-2
title_fullStr Carrageenan from Gigartina skottsbergii: A Novel Molecular Probe to Detect SARS-CoV-2
title_full_unstemmed Carrageenan from Gigartina skottsbergii: A Novel Molecular Probe to Detect SARS-CoV-2
title_short Carrageenan from Gigartina skottsbergii: A Novel Molecular Probe to Detect SARS-CoV-2
title_sort carrageenan from gigartina skottsbergii: a novel molecular probe to detect sars-cov-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10046870/
https://www.ncbi.nlm.nih.gov/pubmed/36979590
http://dx.doi.org/10.3390/bios13030378
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