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Mechanistic Modelling of Biomass Growth, Glucose Consumption and Ethanol Production by Kluyveromyces marxianus in Batch Fermentation

This paper presents results concerning mechanistic modeling to describe the dynamics and interactions between biomass growth, glucose consumption and ethanol production in batch culture fermentation by Kluyveromyces marxianus (K. marxianus). The mathematical model was formulated based on the biologi...

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Autores principales: Salazar, Yolocuauhtli, Valle, Paul A., Rodríguez, Emmanuel, Soto-Cruz, Nicolás O., Páez-Lerma, Jesús B., Reyes-Sánchez, Francisco J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10047689/
https://www.ncbi.nlm.nih.gov/pubmed/36981385
http://dx.doi.org/10.3390/e25030497
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author Salazar, Yolocuauhtli
Valle, Paul A.
Rodríguez, Emmanuel
Soto-Cruz, Nicolás O.
Páez-Lerma, Jesús B.
Reyes-Sánchez, Francisco J.
author_facet Salazar, Yolocuauhtli
Valle, Paul A.
Rodríguez, Emmanuel
Soto-Cruz, Nicolás O.
Páez-Lerma, Jesús B.
Reyes-Sánchez, Francisco J.
author_sort Salazar, Yolocuauhtli
collection PubMed
description This paper presents results concerning mechanistic modeling to describe the dynamics and interactions between biomass growth, glucose consumption and ethanol production in batch culture fermentation by Kluyveromyces marxianus (K. marxianus). The mathematical model was formulated based on the biological assumptions underlying each variable and is given by a set of three coupled nonlinear first-order Ordinary Differential Equations. The model has ten parameters, and their values were fitted from the experimental data of 17 K. marxianus strains by means of a computational algorithm design in Matlab. The latter allowed us to determine that seven of these parameters share the same value among all the strains, while three parameters concerning biomass maximum growth rate, and ethanol production due to biomass and glucose had specific values for each strain. These values are presented with their corresponding standard error and [Formula: see text] confidence interval. The goodness of fit of our system was evaluated both qualitatively by in silico experimentation and quantitative by means of the coefficient of determination and the Akaike Information Criterion. Results regarding the fitting capabilities were compared with the classic model given by the logistic, Pirt, and Luedeking–Piret Equations. Further, nonlinear theories were applied to investigate local and global dynamics of the system, the Localization of Compact Invariant Sets Method was applied to determine the so-called localizing domain, i.e., lower and upper bounds for each variable; whilst Lyapunov’s stability theories allowed to establish sufficient conditions to ensure asymptotic stability in the nonnegative octant, i.e., [Formula: see text]. Finally, the predictive ability of our mechanistic model was explored through several numerical simulations with expected results according to microbiology literature on batch fermentation.
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spelling pubmed-100476892023-03-29 Mechanistic Modelling of Biomass Growth, Glucose Consumption and Ethanol Production by Kluyveromyces marxianus in Batch Fermentation Salazar, Yolocuauhtli Valle, Paul A. Rodríguez, Emmanuel Soto-Cruz, Nicolás O. Páez-Lerma, Jesús B. Reyes-Sánchez, Francisco J. Entropy (Basel) Article This paper presents results concerning mechanistic modeling to describe the dynamics and interactions between biomass growth, glucose consumption and ethanol production in batch culture fermentation by Kluyveromyces marxianus (K. marxianus). The mathematical model was formulated based on the biological assumptions underlying each variable and is given by a set of three coupled nonlinear first-order Ordinary Differential Equations. The model has ten parameters, and their values were fitted from the experimental data of 17 K. marxianus strains by means of a computational algorithm design in Matlab. The latter allowed us to determine that seven of these parameters share the same value among all the strains, while three parameters concerning biomass maximum growth rate, and ethanol production due to biomass and glucose had specific values for each strain. These values are presented with their corresponding standard error and [Formula: see text] confidence interval. The goodness of fit of our system was evaluated both qualitatively by in silico experimentation and quantitative by means of the coefficient of determination and the Akaike Information Criterion. Results regarding the fitting capabilities were compared with the classic model given by the logistic, Pirt, and Luedeking–Piret Equations. Further, nonlinear theories were applied to investigate local and global dynamics of the system, the Localization of Compact Invariant Sets Method was applied to determine the so-called localizing domain, i.e., lower and upper bounds for each variable; whilst Lyapunov’s stability theories allowed to establish sufficient conditions to ensure asymptotic stability in the nonnegative octant, i.e., [Formula: see text]. Finally, the predictive ability of our mechanistic model was explored through several numerical simulations with expected results according to microbiology literature on batch fermentation. MDPI 2023-03-14 /pmc/articles/PMC10047689/ /pubmed/36981385 http://dx.doi.org/10.3390/e25030497 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Salazar, Yolocuauhtli
Valle, Paul A.
Rodríguez, Emmanuel
Soto-Cruz, Nicolás O.
Páez-Lerma, Jesús B.
Reyes-Sánchez, Francisco J.
Mechanistic Modelling of Biomass Growth, Glucose Consumption and Ethanol Production by Kluyveromyces marxianus in Batch Fermentation
title Mechanistic Modelling of Biomass Growth, Glucose Consumption and Ethanol Production by Kluyveromyces marxianus in Batch Fermentation
title_full Mechanistic Modelling of Biomass Growth, Glucose Consumption and Ethanol Production by Kluyveromyces marxianus in Batch Fermentation
title_fullStr Mechanistic Modelling of Biomass Growth, Glucose Consumption and Ethanol Production by Kluyveromyces marxianus in Batch Fermentation
title_full_unstemmed Mechanistic Modelling of Biomass Growth, Glucose Consumption and Ethanol Production by Kluyveromyces marxianus in Batch Fermentation
title_short Mechanistic Modelling of Biomass Growth, Glucose Consumption and Ethanol Production by Kluyveromyces marxianus in Batch Fermentation
title_sort mechanistic modelling of biomass growth, glucose consumption and ethanol production by kluyveromyces marxianus in batch fermentation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10047689/
https://www.ncbi.nlm.nih.gov/pubmed/36981385
http://dx.doi.org/10.3390/e25030497
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