Cargando…
Cell-Free DNA in Plasma and Serum Indicates Disease Severity and Prognosis in Blunt Trauma Patients
Background: Trauma is still a major cause of mortality in people < 50 years of age. Biomarkers are needed to estimate the severity of the condition and the patient outcome. Methods: Cell-free DNA (cfDNA) and further laboratory markers were determined in plasma and serum of 164 patients at time of...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10047705/ https://www.ncbi.nlm.nih.gov/pubmed/36980458 http://dx.doi.org/10.3390/diagnostics13061150 |
_version_ | 1785013993137504256 |
---|---|
author | Trulson, Inga Stahl, Juliane Margraf, Stefan Scholz, Martin Hoecherl, Eduard Wolf, Konrad Durner, Juergen Klawonn, Frank Holdenrieder, Stefan |
author_facet | Trulson, Inga Stahl, Juliane Margraf, Stefan Scholz, Martin Hoecherl, Eduard Wolf, Konrad Durner, Juergen Klawonn, Frank Holdenrieder, Stefan |
author_sort | Trulson, Inga |
collection | PubMed |
description | Background: Trauma is still a major cause of mortality in people < 50 years of age. Biomarkers are needed to estimate the severity of the condition and the patient outcome. Methods: Cell-free DNA (cfDNA) and further laboratory markers were determined in plasma and serum of 164 patients at time of admission to the emergency room. Among them were 64 patients with severe trauma (Injury Severity Score (ISS) ≥ 16), 51 patients with moderate trauma (ISS < 16) and 49 patients with single fractures (24 femur neck and 25 ankle fractures). Disease severity was objectified by ISS and Glasgow Coma Scale (GCS). Results: cfDNA levels in plasma and serum were significantly higher in patients with severe multiple trauma (SMT) than in those with moderate trauma (p = 0.002, p = 0.003, respectively) or with single fractures (each p < 0.001). CfDNA in plasma and serum correlated very strongly with each other (R = 0.91; p < 0.001). The AUC in ROC curves for identification of SMT patients was 0.76 and 0.74 for cfDNA in plasma and serum, respectively—this was further increased to 0.84 by the combination of cfDNA and hemoglobin. Within the group of multiple trauma patients, cfDNA levels were significantly higher in more severely injured patients and patients with severe traumatic brain injury (GCS ≤ 8 versus GCS > 8). Thirteen (20.3%) of the multiple trauma patients died during the first week after trauma. Levels of cfDNA were significantly higher in non-surviving patients than in survivors (p < 0.001), reaching an AUC of 0.81 for cfDNA in both, plasma and serum, which was further increased by the combination with hemoglobin and leukocytes. Conclusions: cfDNA is valuable for estimation of trauma severity and prognosis of trauma patients. |
format | Online Article Text |
id | pubmed-10047705 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100477052023-03-29 Cell-Free DNA in Plasma and Serum Indicates Disease Severity and Prognosis in Blunt Trauma Patients Trulson, Inga Stahl, Juliane Margraf, Stefan Scholz, Martin Hoecherl, Eduard Wolf, Konrad Durner, Juergen Klawonn, Frank Holdenrieder, Stefan Diagnostics (Basel) Article Background: Trauma is still a major cause of mortality in people < 50 years of age. Biomarkers are needed to estimate the severity of the condition and the patient outcome. Methods: Cell-free DNA (cfDNA) and further laboratory markers were determined in plasma and serum of 164 patients at time of admission to the emergency room. Among them were 64 patients with severe trauma (Injury Severity Score (ISS) ≥ 16), 51 patients with moderate trauma (ISS < 16) and 49 patients with single fractures (24 femur neck and 25 ankle fractures). Disease severity was objectified by ISS and Glasgow Coma Scale (GCS). Results: cfDNA levels in plasma and serum were significantly higher in patients with severe multiple trauma (SMT) than in those with moderate trauma (p = 0.002, p = 0.003, respectively) or with single fractures (each p < 0.001). CfDNA in plasma and serum correlated very strongly with each other (R = 0.91; p < 0.001). The AUC in ROC curves for identification of SMT patients was 0.76 and 0.74 for cfDNA in plasma and serum, respectively—this was further increased to 0.84 by the combination of cfDNA and hemoglobin. Within the group of multiple trauma patients, cfDNA levels were significantly higher in more severely injured patients and patients with severe traumatic brain injury (GCS ≤ 8 versus GCS > 8). Thirteen (20.3%) of the multiple trauma patients died during the first week after trauma. Levels of cfDNA were significantly higher in non-surviving patients than in survivors (p < 0.001), reaching an AUC of 0.81 for cfDNA in both, plasma and serum, which was further increased by the combination with hemoglobin and leukocytes. Conclusions: cfDNA is valuable for estimation of trauma severity and prognosis of trauma patients. MDPI 2023-03-17 /pmc/articles/PMC10047705/ /pubmed/36980458 http://dx.doi.org/10.3390/diagnostics13061150 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Trulson, Inga Stahl, Juliane Margraf, Stefan Scholz, Martin Hoecherl, Eduard Wolf, Konrad Durner, Juergen Klawonn, Frank Holdenrieder, Stefan Cell-Free DNA in Plasma and Serum Indicates Disease Severity and Prognosis in Blunt Trauma Patients |
title | Cell-Free DNA in Plasma and Serum Indicates Disease Severity and Prognosis in Blunt Trauma Patients |
title_full | Cell-Free DNA in Plasma and Serum Indicates Disease Severity and Prognosis in Blunt Trauma Patients |
title_fullStr | Cell-Free DNA in Plasma and Serum Indicates Disease Severity and Prognosis in Blunt Trauma Patients |
title_full_unstemmed | Cell-Free DNA in Plasma and Serum Indicates Disease Severity and Prognosis in Blunt Trauma Patients |
title_short | Cell-Free DNA in Plasma and Serum Indicates Disease Severity and Prognosis in Blunt Trauma Patients |
title_sort | cell-free dna in plasma and serum indicates disease severity and prognosis in blunt trauma patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10047705/ https://www.ncbi.nlm.nih.gov/pubmed/36980458 http://dx.doi.org/10.3390/diagnostics13061150 |
work_keys_str_mv | AT trulsoninga cellfreednainplasmaandserumindicatesdiseaseseverityandprognosisinblunttraumapatients AT stahljuliane cellfreednainplasmaandserumindicatesdiseaseseverityandprognosisinblunttraumapatients AT margrafstefan cellfreednainplasmaandserumindicatesdiseaseseverityandprognosisinblunttraumapatients AT scholzmartin cellfreednainplasmaandserumindicatesdiseaseseverityandprognosisinblunttraumapatients AT hoecherleduard cellfreednainplasmaandserumindicatesdiseaseseverityandprognosisinblunttraumapatients AT wolfkonrad cellfreednainplasmaandserumindicatesdiseaseseverityandprognosisinblunttraumapatients AT durnerjuergen cellfreednainplasmaandserumindicatesdiseaseseverityandprognosisinblunttraumapatients AT klawonnfrank cellfreednainplasmaandserumindicatesdiseaseseverityandprognosisinblunttraumapatients AT holdenriederstefan cellfreednainplasmaandserumindicatesdiseaseseverityandprognosisinblunttraumapatients |