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Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells

Acrylamide (AA) and ochratoxin A (OTA) are contaminants that co-exist in the same foods, and may create a serious threat to human health. However, the combined effects of AA and OTA on intestinal epithelial cells remain unclear. The purpose of this research was to investigate the effects of AA and O...

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Autores principales: Su, Dan, Lu, Jiawen, Nie, Chunchao, Guo, Ziyan, Li, Chang, Yu, Qiang, Xie, Jianhua, Chen, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048136/
https://www.ncbi.nlm.nih.gov/pubmed/36981244
http://dx.doi.org/10.3390/foods12061318
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author Su, Dan
Lu, Jiawen
Nie, Chunchao
Guo, Ziyan
Li, Chang
Yu, Qiang
Xie, Jianhua
Chen, Yi
author_facet Su, Dan
Lu, Jiawen
Nie, Chunchao
Guo, Ziyan
Li, Chang
Yu, Qiang
Xie, Jianhua
Chen, Yi
author_sort Su, Dan
collection PubMed
description Acrylamide (AA) and ochratoxin A (OTA) are contaminants that co-exist in the same foods, and may create a serious threat to human health. However, the combined effects of AA and OTA on intestinal epithelial cells remain unclear. The purpose of this research was to investigate the effects of AA and OTA individually and collectively on Caco-2 cells. The results showed that AA and OTA significantly inhibited Caco-2 cell viability in a concentration- and time-dependent manner, decreased transepithelial electrical resistance (TEER) values, and increased the lucifer yellow (LY) permeabilization, lactate dehydrogenase (LDH) release and reactive oxygen species (ROS) levels. In addition, the levels of IL-1β, IL-6, and TNF-α increased, while the levels of IL-10 decreased after AA and OTA treatment. Western blot analysis revealed that AA and OTA damaged the intestinal barrier by reducing the expression of the tight junction (TJ) protein. The collective effects of AA and OTA exhibited enhanced toxicity compared to either single compound and, for most of the intestinal barrier function indicators, AA and OTA combined exposure tended to produce synergistic toxicity to Caco-2 cells. Overall, this research suggests the possibility of toxic reactions arising from the interaction of toxic substances present in foodstuffs with those produced during processing.
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spelling pubmed-100481362023-03-29 Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells Su, Dan Lu, Jiawen Nie, Chunchao Guo, Ziyan Li, Chang Yu, Qiang Xie, Jianhua Chen, Yi Foods Article Acrylamide (AA) and ochratoxin A (OTA) are contaminants that co-exist in the same foods, and may create a serious threat to human health. However, the combined effects of AA and OTA on intestinal epithelial cells remain unclear. The purpose of this research was to investigate the effects of AA and OTA individually and collectively on Caco-2 cells. The results showed that AA and OTA significantly inhibited Caco-2 cell viability in a concentration- and time-dependent manner, decreased transepithelial electrical resistance (TEER) values, and increased the lucifer yellow (LY) permeabilization, lactate dehydrogenase (LDH) release and reactive oxygen species (ROS) levels. In addition, the levels of IL-1β, IL-6, and TNF-α increased, while the levels of IL-10 decreased after AA and OTA treatment. Western blot analysis revealed that AA and OTA damaged the intestinal barrier by reducing the expression of the tight junction (TJ) protein. The collective effects of AA and OTA exhibited enhanced toxicity compared to either single compound and, for most of the intestinal barrier function indicators, AA and OTA combined exposure tended to produce synergistic toxicity to Caco-2 cells. Overall, this research suggests the possibility of toxic reactions arising from the interaction of toxic substances present in foodstuffs with those produced during processing. MDPI 2023-03-20 /pmc/articles/PMC10048136/ /pubmed/36981244 http://dx.doi.org/10.3390/foods12061318 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Su, Dan
Lu, Jiawen
Nie, Chunchao
Guo, Ziyan
Li, Chang
Yu, Qiang
Xie, Jianhua
Chen, Yi
Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells
title Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells
title_full Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells
title_fullStr Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells
title_full_unstemmed Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells
title_short Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells
title_sort combined effects of acrylamide and ochratoxin a on the intestinal barrier in caco-2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048136/
https://www.ncbi.nlm.nih.gov/pubmed/36981244
http://dx.doi.org/10.3390/foods12061318
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