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Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells
Acrylamide (AA) and ochratoxin A (OTA) are contaminants that co-exist in the same foods, and may create a serious threat to human health. However, the combined effects of AA and OTA on intestinal epithelial cells remain unclear. The purpose of this research was to investigate the effects of AA and O...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048136/ https://www.ncbi.nlm.nih.gov/pubmed/36981244 http://dx.doi.org/10.3390/foods12061318 |
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author | Su, Dan Lu, Jiawen Nie, Chunchao Guo, Ziyan Li, Chang Yu, Qiang Xie, Jianhua Chen, Yi |
author_facet | Su, Dan Lu, Jiawen Nie, Chunchao Guo, Ziyan Li, Chang Yu, Qiang Xie, Jianhua Chen, Yi |
author_sort | Su, Dan |
collection | PubMed |
description | Acrylamide (AA) and ochratoxin A (OTA) are contaminants that co-exist in the same foods, and may create a serious threat to human health. However, the combined effects of AA and OTA on intestinal epithelial cells remain unclear. The purpose of this research was to investigate the effects of AA and OTA individually and collectively on Caco-2 cells. The results showed that AA and OTA significantly inhibited Caco-2 cell viability in a concentration- and time-dependent manner, decreased transepithelial electrical resistance (TEER) values, and increased the lucifer yellow (LY) permeabilization, lactate dehydrogenase (LDH) release and reactive oxygen species (ROS) levels. In addition, the levels of IL-1β, IL-6, and TNF-α increased, while the levels of IL-10 decreased after AA and OTA treatment. Western blot analysis revealed that AA and OTA damaged the intestinal barrier by reducing the expression of the tight junction (TJ) protein. The collective effects of AA and OTA exhibited enhanced toxicity compared to either single compound and, for most of the intestinal barrier function indicators, AA and OTA combined exposure tended to produce synergistic toxicity to Caco-2 cells. Overall, this research suggests the possibility of toxic reactions arising from the interaction of toxic substances present in foodstuffs with those produced during processing. |
format | Online Article Text |
id | pubmed-10048136 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100481362023-03-29 Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells Su, Dan Lu, Jiawen Nie, Chunchao Guo, Ziyan Li, Chang Yu, Qiang Xie, Jianhua Chen, Yi Foods Article Acrylamide (AA) and ochratoxin A (OTA) are contaminants that co-exist in the same foods, and may create a serious threat to human health. However, the combined effects of AA and OTA on intestinal epithelial cells remain unclear. The purpose of this research was to investigate the effects of AA and OTA individually and collectively on Caco-2 cells. The results showed that AA and OTA significantly inhibited Caco-2 cell viability in a concentration- and time-dependent manner, decreased transepithelial electrical resistance (TEER) values, and increased the lucifer yellow (LY) permeabilization, lactate dehydrogenase (LDH) release and reactive oxygen species (ROS) levels. In addition, the levels of IL-1β, IL-6, and TNF-α increased, while the levels of IL-10 decreased after AA and OTA treatment. Western blot analysis revealed that AA and OTA damaged the intestinal barrier by reducing the expression of the tight junction (TJ) protein. The collective effects of AA and OTA exhibited enhanced toxicity compared to either single compound and, for most of the intestinal barrier function indicators, AA and OTA combined exposure tended to produce synergistic toxicity to Caco-2 cells. Overall, this research suggests the possibility of toxic reactions arising from the interaction of toxic substances present in foodstuffs with those produced during processing. MDPI 2023-03-20 /pmc/articles/PMC10048136/ /pubmed/36981244 http://dx.doi.org/10.3390/foods12061318 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Su, Dan Lu, Jiawen Nie, Chunchao Guo, Ziyan Li, Chang Yu, Qiang Xie, Jianhua Chen, Yi Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells |
title | Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells |
title_full | Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells |
title_fullStr | Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells |
title_full_unstemmed | Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells |
title_short | Combined Effects of Acrylamide and Ochratoxin A on the Intestinal Barrier in Caco-2 Cells |
title_sort | combined effects of acrylamide and ochratoxin a on the intestinal barrier in caco-2 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048136/ https://www.ncbi.nlm.nih.gov/pubmed/36981244 http://dx.doi.org/10.3390/foods12061318 |
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